Vol 2, No 4 (2001): Practical Diabetology
Research paper
Published online: 2001-11-19
Tumor necrosis factor alpha (TNF- < font face=
Diabetologia Praktyczna 2001;2(4):267-272.
Abstract
INTRODUCTION. Tumor necrosis factor a (TNF-a) is
a cytokine which may contribute to the pathogenesis of insulin resistance. This resistance to insulin action plays a key role in type 2 diabetes. The aim
of the study was the assessment of the association of TNF-a gene polymorphism with type 2 diabetes in Polish population.
MATERIAL AND METHODS. G-308A polymorphism in the promoter region of TNF-a gene was detected using polymerase chain reaction and restriction fragments length polymorphism (PCR-RFLP) method. Studied subjects were 172 patients with type 2 diabetes. Control group consisted of 172 healthy individuals with normal glucose tolerance, age and sex matched to study group.
RESULTS. TNF2 genotype occurred more frequently in healthy individuals when compared to diabetics (0.29 vs. 0.20). Diabetic TNF2 genotype bearers clinical chacharacteristics did not differ substantially to this of diabetics with TNF1 genotype. The only important difference was higher frequency of arterial hypertension in TNF2 diabetic patients comparing to normotensive diabetic individuals. There was statistically important difference in TNF2 frequency, which was substantially lower among normotensive diabetics than among diabetics with hypertension and control subjects (p = 0.011).
CONCLUSIONS. TNF2 genotype seems not to be associated with susceptibility to type 2 diabetes but in contrary might be protective against the disease. TNF2 variant is a risk factor of arterial hypertension accompanying type 2 diabetes in study population.
MATERIAL AND METHODS. G-308A polymorphism in the promoter region of TNF-a gene was detected using polymerase chain reaction and restriction fragments length polymorphism (PCR-RFLP) method. Studied subjects were 172 patients with type 2 diabetes. Control group consisted of 172 healthy individuals with normal glucose tolerance, age and sex matched to study group.
RESULTS. TNF2 genotype occurred more frequently in healthy individuals when compared to diabetics (0.29 vs. 0.20). Diabetic TNF2 genotype bearers clinical chacharacteristics did not differ substantially to this of diabetics with TNF1 genotype. The only important difference was higher frequency of arterial hypertension in TNF2 diabetic patients comparing to normotensive diabetic individuals. There was statistically important difference in TNF2 frequency, which was substantially lower among normotensive diabetics than among diabetics with hypertension and control subjects (p = 0.011).
CONCLUSIONS. TNF2 genotype seems not to be associated with susceptibility to type 2 diabetes but in contrary might be protective against the disease. TNF2 variant is a risk factor of arterial hypertension accompanying type 2 diabetes in study population.
Keywords: tumor necrosis factor-ainsulin resistancediabetes type 2arterial hypertensiongenetic polymorphism