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Vol 1, No 2 (2012)
Original articles (submitted)
Published online: 2012-06-01
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Blood serum of patients with diabetic kidney disease stimulates thrombospondin 1 gene expression in peripheral blood mononuclear cells

Dariusz Moczulski, Irmina Korzeniewska-Dyl, Konrad Walczak
Diabetologia Kliniczna 2012;1(2):55-60.

open access

Vol 1, No 2 (2012)
Original articles (submitted)
Published online: 2012-06-01

Abstract

Background. The thrombospondin 1 and cyclooxygenase1 genes were over-expressed and the matrixmetalloproteinase 9 and cyclooxygenase 2 geneshave lower expression in peripheral blood mononuclearcells (PBMC) of type 1 diabetic patients with diabetic kidney disease (DKD).

The aim of the study wasto examine if the expression of those genes couldbe stimulate by incubating PBMC of healthy volunteersin blood serum of patients with DKD.

Material and methods. Blood serum samples fromfive type 2 diabetic patients with DKD and five type2 diabetic patients without DKD were collected.PBMC of four healthy volunteers were incubated inblood serum of 5 type 2 diabetic patients with DKDand 5 without DKD. The expression of the thrombospondin1, cyclooxygenase 1, matrix metalloproteinase9 and cyclooxygenase 2 genes was examinedusing a method based on the real-time PCR. Theexpression was compared between cells incubated in blood serum of type 2 diabetic patients with andwithout DKD.

Results. We observed a higher expression of thethrombospondin 1 gene in cells incubated in bloodserum of patients with DKD. The expression of othergenes did not differ between cells incubated in bloodserum of patients with and without DKD.

Conclusions. Blood serum of type 2 diabetic patientswith DKD induces expression of thrombospondin 1gene in PBMC. Blood serum of type 2 diabetespatients with DKD may contain an intrinsic factorwhich may be involved in vascular complicationsaccelerated by DKD.

Abstract

Background. The thrombospondin 1 and cyclooxygenase1 genes were over-expressed and the matrixmetalloproteinase 9 and cyclooxygenase 2 geneshave lower expression in peripheral blood mononuclearcells (PBMC) of type 1 diabetic patients with diabetic kidney disease (DKD).

The aim of the study wasto examine if the expression of those genes couldbe stimulate by incubating PBMC of healthy volunteersin blood serum of patients with DKD.

Material and methods. Blood serum samples fromfive type 2 diabetic patients with DKD and five type2 diabetic patients without DKD were collected.PBMC of four healthy volunteers were incubated inblood serum of 5 type 2 diabetic patients with DKDand 5 without DKD. The expression of the thrombospondin1, cyclooxygenase 1, matrix metalloproteinase9 and cyclooxygenase 2 genes was examinedusing a method based on the real-time PCR. Theexpression was compared between cells incubated in blood serum of type 2 diabetic patients with andwithout DKD.

Results. We observed a higher expression of thethrombospondin 1 gene in cells incubated in bloodserum of patients with DKD. The expression of othergenes did not differ between cells incubated in bloodserum of patients with and without DKD.

Conclusions. Blood serum of type 2 diabetic patientswith DKD induces expression of thrombospondin 1gene in PBMC. Blood serum of type 2 diabetespatients with DKD may contain an intrinsic factorwhich may be involved in vascular complicationsaccelerated by DKD.

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Keywords

diabetic kidney disease, gene expression, peripheral blood mononuclear cells, blood serum, thrombospondin 1, type 2 diabetes

About this article
Title

Blood serum of patients with diabetic kidney disease stimulates thrombospondin 1 gene expression in peripheral blood mononuclear cells

Journal

Clinical Diabetology

Issue

Vol 1, No 2 (2012)

Pages

55-60

Published online

2012-06-01

Bibliographic record

Diabetologia Kliniczna 2012;1(2):55-60.

Keywords

diabetic kidney disease
gene expression
peripheral blood mononuclear cells
blood serum
thrombospondin 1
type 2 diabetes

Authors

Dariusz Moczulski
Irmina Korzeniewska-Dyl
Konrad Walczak

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