open access

Vol 16, No 1 (2014)
Original articles
Published online: 2014-11-13
Get Citation

Assessment of TNF-a and collagen type I and III expression in the gastric carcinoma tissue

Robert Partyka, Daniel Knap, Grażyna Biedrzyńska-Macyszyn, Janusz Kobiela, Przemysław Jałowiecki, Danuta Kokocińska
Chirurgia Polska 2014;16(1):28-35.

open access

Vol 16, No 1 (2014)
Original articles
Published online: 2014-11-13

Abstract

Introduction: Neoplasms are the second most frequent cause of death in Poland after vascular diseases. Recently, gastric cancer morbidity has decreased, but mortality is steel at a high level.

Material and methods: Tissues from 24 patients with a histopathologically diagnosed mucosal and adenomucosal gastric cancer have been tested. Patients were divided in two equal groups: patients without metastases (G1) and patients with metastases in the liver (G2). RNA was isolated from homogenates after preliminary powdering of tissues in liquid nitrogen. RNA concentration was determined using spectrophotometer with RNA/DNA calculator ’Gene Quant’ (LKB Pharmacia Biotech). The tissue expression of the TNF-a, collagen type I and collagen type II encoding genes were assessed.

Results: Higher TNF-a RNA expression was observed only for 2 patients from group G1, while in G2 group higher expression was observed in 5 from 12 patients in comparison to mean expression in margin. Higher expression of collagen I was found in 6 of 12 patients from the G1 group in comparison to margin. In the tissues with metastases (G2) higher expression of collagen type I was found in 5 tested tissues in comparison to margin. Higher expression of collagen III gene in comparison to margin was found in 8 from 12 tested tissues from G1 group. Higher expression in group 2 was also observed in 8 out of 12 tissues, in comparison to margin value in group I. Correlation analysis showed correlation between collagen I and TNF-a only in the healthy tissue (r = –0.603; p < 0.05).

Conclusions. A statistically non-significant increase in expression of the TNF-a coding genes, type I and III collagen and lack of their mutual correlation weighs in favor of the fact that they are independent parameters. A statistically non-significant increase in expression of the TNF-a and collagen type I coding genes and a negative correlation between them indicates that TNF can’t affect the synthesis of collagen I.

Abstract

Introduction: Neoplasms are the second most frequent cause of death in Poland after vascular diseases. Recently, gastric cancer morbidity has decreased, but mortality is steel at a high level.

Material and methods: Tissues from 24 patients with a histopathologically diagnosed mucosal and adenomucosal gastric cancer have been tested. Patients were divided in two equal groups: patients without metastases (G1) and patients with metastases in the liver (G2). RNA was isolated from homogenates after preliminary powdering of tissues in liquid nitrogen. RNA concentration was determined using spectrophotometer with RNA/DNA calculator ’Gene Quant’ (LKB Pharmacia Biotech). The tissue expression of the TNF-a, collagen type I and collagen type II encoding genes were assessed.

Results: Higher TNF-a RNA expression was observed only for 2 patients from group G1, while in G2 group higher expression was observed in 5 from 12 patients in comparison to mean expression in margin. Higher expression of collagen I was found in 6 of 12 patients from the G1 group in comparison to margin. In the tissues with metastases (G2) higher expression of collagen type I was found in 5 tested tissues in comparison to margin. Higher expression of collagen III gene in comparison to margin was found in 8 from 12 tested tissues from G1 group. Higher expression in group 2 was also observed in 8 out of 12 tissues, in comparison to margin value in group I. Correlation analysis showed correlation between collagen I and TNF-a only in the healthy tissue (r = –0.603; p < 0.05).

Conclusions. A statistically non-significant increase in expression of the TNF-a coding genes, type I and III collagen and lack of their mutual correlation weighs in favor of the fact that they are independent parameters. A statistically non-significant increase in expression of the TNF-a and collagen type I coding genes and a negative correlation between them indicates that TNF can’t affect the synthesis of collagen I.

Get Citation

Keywords

TNF-a, collagen I, collagen III, gastric cancer

About this article
Title

Assessment of TNF-a and collagen type I and III expression in the gastric carcinoma tissue

Journal

Chirurgia Polska (Polish Surgery)

Issue

Vol 16, No 1 (2014)

Pages

28-35

Published online

2014-11-13

Bibliographic record

Chirurgia Polska 2014;16(1):28-35.

Keywords

TNF-a
collagen I
collagen III
gastric cancer

Authors

Robert Partyka
Daniel Knap
Grażyna Biedrzyńska-Macyszyn
Janusz Kobiela
Przemysław Jałowiecki
Danuta Kokocińska

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

Via MedicaBy "Via Medica sp. z o.o." sp.k., ul. Świętokrzyska 73, 80–180 Gdańsk

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail: viamedica@viamedica.pl