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Vol 4, No 3 (2002)
Published online: 2002-11-08
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Assessment of the mechanical proprieties of human allogenic arteries after antibiotic sterilisation and cryopreservation

Tomasz Urbanek, Antoni Wala, Henryk Bursig, Krzysztof Ziaja, Wacław Kuczmik
Chirurgia Polska 2002;4(3):117-124.

open access

Vol 4, No 3 (2002)
Published online: 2002-11-08

Abstract

Background: Assessment of the mechanical properties of human arteries after cryopreservation processing protocol including antibiotic sterilisation and controlled freezing with the use of cryoprotector (10% DMSO).
Material and methods: 12 aortic and 9 femoral artery segments were investigated; the donor age ranged from 18 to 42 years, the time of the storage in the liquid nitrogen vapours was from 1 to 36 months. The stretching tests of vessel specimens (using INSTRON tensile testing machine) and manometric investigations were performed. The mechanical resistance (breaking stress) and elastic moduli were evaluated (at maximal stress value and at the stress of 300 kPa). The results were compared with fresh homograft specimens.
Results: The mechanical resistance (breaking stress) of the investigated cryopreserved aortic segments ranged from 1.5 MPa (± 0.34) (if stretched perpendicular to the vessel long axis) to 1.05 MPa (± 0.3) (if stretching was performed in parallel). For the femoral artery specimens the respective values were 2.4 MPa (± 0.93) and 1.35 MPa (± 0.5). Elastic moduli at the stress of 300 kPa in aortic specimens achieved 1.38 (± 0.4) and 1.8 (± 0.85). For the attained femoral arteries respective values were 2.83 (± 1.34) and 1.47 (± 0.9). There were no significant differences concerning mechanical properties between fresh and cryopreserved aortic wall segments.
Conclusions: 1. Cryopreserved allogenic arteries (aorta, femoral arteries) maintain high mechanical resistance against breaking stress and intra-arterial pressure. 2. Despite vessel preparation (including sterilisation and control freezing protocols), cryopreserved arteries maintain elastic properties, justifying an implementation of this material in human circulatory system. 3. The use of arterial segments with low number of collaterals and their correct suture or ligation can influence the allograft mechanical stress resistance.

Abstract

Background: Assessment of the mechanical properties of human arteries after cryopreservation processing protocol including antibiotic sterilisation and controlled freezing with the use of cryoprotector (10% DMSO).
Material and methods: 12 aortic and 9 femoral artery segments were investigated; the donor age ranged from 18 to 42 years, the time of the storage in the liquid nitrogen vapours was from 1 to 36 months. The stretching tests of vessel specimens (using INSTRON tensile testing machine) and manometric investigations were performed. The mechanical resistance (breaking stress) and elastic moduli were evaluated (at maximal stress value and at the stress of 300 kPa). The results were compared with fresh homograft specimens.
Results: The mechanical resistance (breaking stress) of the investigated cryopreserved aortic segments ranged from 1.5 MPa (± 0.34) (if stretched perpendicular to the vessel long axis) to 1.05 MPa (± 0.3) (if stretching was performed in parallel). For the femoral artery specimens the respective values were 2.4 MPa (± 0.93) and 1.35 MPa (± 0.5). Elastic moduli at the stress of 300 kPa in aortic specimens achieved 1.38 (± 0.4) and 1.8 (± 0.85). For the attained femoral arteries respective values were 2.83 (± 1.34) and 1.47 (± 0.9). There were no significant differences concerning mechanical properties between fresh and cryopreserved aortic wall segments.
Conclusions: 1. Cryopreserved allogenic arteries (aorta, femoral arteries) maintain high mechanical resistance against breaking stress and intra-arterial pressure. 2. Despite vessel preparation (including sterilisation and control freezing protocols), cryopreserved arteries maintain elastic properties, justifying an implementation of this material in human circulatory system. 3. The use of arterial segments with low number of collaterals and their correct suture or ligation can influence the allograft mechanical stress resistance.
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Keywords

homograft; cryopreservation; mechanical proprieties; tensil strength; vascular surgery; aorta; femoral artery

About this article
Title

Assessment of the mechanical proprieties of human allogenic arteries after antibiotic sterilisation and cryopreservation

Journal

Chirurgia Polska (Polish Surgery)

Issue

Vol 4, No 3 (2002)

Pages

117-124

Published online

2002-11-08

Bibliographic record

Chirurgia Polska 2002;4(3):117-124.

Keywords

homograft
cryopreservation
mechanical proprieties
tensil strength
vascular surgery
aorta
femoral artery

Authors

Tomasz Urbanek
Antoni Wala
Henryk Bursig
Krzysztof Ziaja
Wacław Kuczmik

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