open access

Vol 6, No 4 (2004)
Published online: 2005-01-26
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Coronary arteries in Sprague-Dawley rats visualized by means of gradual corrosion

Konstanty Ślusarczyk, Stanisław Skrzelewski, Jerzy Nożyński, Agnieszka Kędzia, Maurycy Porc, Brygida Beck-Ciszek, Tadeusz F. Krzemiński
Chirurgia Polska 2004;6(4):239-244.

open access

Vol 6, No 4 (2004)
Published online: 2005-01-26

Abstract

Backgrounds: The subject of the study were the coronary arteries of Sprague-Dawley rats visualized by means of gradual corrosion.
Material and methods: Investigations were carried out on 20 adult male animals. The agreement of the Local Ethical Committee was obtained. After deep anaesthesia the chest organs were taken out. The heart was perfused (Langendorff’s method, Krebs-Henseleit solution). Then latex (LKBS 54–50) was applied, the specimen was being fixed in Bouin solution for 4 days. Then the heart was digested in HCl. Before corrosion started, photographs were taken and outlines made. These procedures were repeated during the process of corrosion which lasted for 4 days. The digestion was interrupted when small vascular branches were visualized with the heart ventricle walls still present.
Results: In Sprague-Dawley rats there are two coronary arteries — left and right, both arising from the ascending aorta. They supply ventricles and partly the atria which are supplied also by the extracoronary vessels. About 3–4 mm from its origin, one of the coronary arteries gives rise to a strong anterior interventricular branch (named also a septal artery). It arises from the right coronary artery in 16 (80%) cases, and from the left in 4 (20%).
Conlusions: The characteristic feature of rat coronary arteries and their branches is their wave-like course, partly deep or superficial. The smallest twigs of both of the coronary and septal arteries run deeply intramuscularly and give off many shaggy configurated vessels of a terminal character.
Many anastomoses were observed between branches of the ipsilateral (more frequent) and contralateral (rare) arteries. As an exception, there are the anastomoses between the smallest twigs of all the abovementioned vessels.

Abstract

Backgrounds: The subject of the study were the coronary arteries of Sprague-Dawley rats visualized by means of gradual corrosion.
Material and methods: Investigations were carried out on 20 adult male animals. The agreement of the Local Ethical Committee was obtained. After deep anaesthesia the chest organs were taken out. The heart was perfused (Langendorff’s method, Krebs-Henseleit solution). Then latex (LKBS 54–50) was applied, the specimen was being fixed in Bouin solution for 4 days. Then the heart was digested in HCl. Before corrosion started, photographs were taken and outlines made. These procedures were repeated during the process of corrosion which lasted for 4 days. The digestion was interrupted when small vascular branches were visualized with the heart ventricle walls still present.
Results: In Sprague-Dawley rats there are two coronary arteries — left and right, both arising from the ascending aorta. They supply ventricles and partly the atria which are supplied also by the extracoronary vessels. About 3–4 mm from its origin, one of the coronary arteries gives rise to a strong anterior interventricular branch (named also a septal artery). It arises from the right coronary artery in 16 (80%) cases, and from the left in 4 (20%).
Conlusions: The characteristic feature of rat coronary arteries and their branches is their wave-like course, partly deep or superficial. The smallest twigs of both of the coronary and septal arteries run deeply intramuscularly and give off many shaggy configurated vessels of a terminal character.
Many anastomoses were observed between branches of the ipsilateral (more frequent) and contralateral (rare) arteries. As an exception, there are the anastomoses between the smallest twigs of all the abovementioned vessels.
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Keywords

Sprague-Dawley rats; coronary arteries; gradual corrosion method

About this article
Title

Coronary arteries in Sprague-Dawley rats visualized by means of gradual corrosion

Journal

Chirurgia Polska (Polish Surgery)

Issue

Vol 6, No 4 (2004)

Pages

239-244

Published online

2005-01-26

Bibliographic record

Chirurgia Polska 2004;6(4):239-244.

Keywords

Sprague-Dawley rats
coronary arteries
gradual corrosion method

Authors

Konstanty Ślusarczyk
Stanisław Skrzelewski
Jerzy Nożyński
Agnieszka Kędzia
Maurycy Porc
Brygida Beck-Ciszek
Tadeusz F. Krzemiński

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