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Vol 7, No 3 (2005)
Published online: 2005-11-07

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Differential diagnosis of gingival hyperplasia based on IFNgamma-stimulated gene expression using RT-PCR

Iwona Niedzielska, Daniel Sypniewski, Daria Wziątek-Kuczmik
Chirurgia Polska 2005;7(3):174-179.


Background: Epulus is a benign gingival tumour of unknown etiopathgenesis. Classification is inconsistent, and standard management strategies are lacking. Epuli are generally believed to be inflammatory rather than neoplastic lesions. The literature does not present any molecular analysis of the tumours’ characteristics. Aim of study: The purpose of the present study was to compare benign (epulus) and malignant (cancer) gingival hyperplasias with regard to the activity of the genes of apoptosis, proliferation, and inflammation using RT-PCR.
Material and methods: The investigation involved 70 patients with epuli and 15 patients with gingival squamous cell carcinoma. Each subject had specimens collected from the tumour, tissue margin (incision line), and healthy tissue. Molecular investigations by RT-PCR were used to evaluate expression levels of the genes of apoptosis (BCL-2, BAX, BCL-2/BAX), proliferation (H3 histone), and inflammatory processes (IFNγ, IFNγR1, IFNγR2, IFNγR1/IFNγR2).
Results: Correlations were discovered between apoptosis and proliferation gene expression in giant cell puli and high-differentiated gingival squamous cell carcinoma.
Conclusions: In RT-PCR molecular analysis, giant cell epulus shows characteristics of a neoplastic lesion while other epulus types seem to be inflammatory tumours.

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