open access

Vol 8, No 3 (2006)
Published online: 2006-08-02
Get Citation

Expression profiles of genes associated with apoptosis assessed by oligonucleotide microarray analysis in advanced coronary artery disease and post-infarction patients

Józefa Dąbek, Zbigniew Gąsior, Przemysław Szmagała, Aleksander Owczarek, Andrzej Kułach, Barbara Monastyrska-Cup, Urszula Mazurek, Andrzej Bochenek
Chirurgia Polska 2006;8(3):184-190.

open access

Vol 8, No 3 (2006)
Published online: 2006-08-02

Abstract

Background: According to recent data, the pathology of cardiomyocyte death during and after myocardial infarction involves both necrosis and apoptosis. Although both mechanisms eventually lead to cell death, the participation of apoptosis in this process carries the potential of developing strategies to influence at least part of the population of dying cells. Therefore, the aim of this study was to determine expression profiles of genes associated with apoptosis in post-infarction myocardium, chronically ischemic myocardium as well as in a healthy heart muscle with the use of oligonucleotide microarrays.
Material and methods: The tissue samples used were sections from the right cardiac auricles obtained during elective surgery performed on 3 patients, as stated above. Then, the expression of 141 genes involved in fibrosis was assessed using an Affymetrix HG_U133A microarray. The similarity of the patients’ transcriptomes was compared using hierarchical clusterisation.
Results: Hierarchical clusterisation demonstrated that the profile of gene expression in post-infarction myocardium was different than that in the remaining specimens. Regression analysis showed that in post-infraction sample genes encoding caspase 4, PDCD8, BBC3, BIT1 and DIABLO were overexpressed, whereas transcriptional activity of 2 genes encoding TNFα receptors presented a lower expression in the post-infarction sample.
Conclusions: The profile of apoptosis-associated genes is significantly different in post-myocardial infarction (post-MI) myocardium compared to chronically ischemic and non-ischemic myocardium. Our data suggest the importance of proapoptotic factors associated with mitochondria. Genes encoding a cascade of caspases were undifferentiating, while 2 genes encoding TNFa receptors were underexpressed in post-infarction tissue.

Abstract

Background: According to recent data, the pathology of cardiomyocyte death during and after myocardial infarction involves both necrosis and apoptosis. Although both mechanisms eventually lead to cell death, the participation of apoptosis in this process carries the potential of developing strategies to influence at least part of the population of dying cells. Therefore, the aim of this study was to determine expression profiles of genes associated with apoptosis in post-infarction myocardium, chronically ischemic myocardium as well as in a healthy heart muscle with the use of oligonucleotide microarrays.
Material and methods: The tissue samples used were sections from the right cardiac auricles obtained during elective surgery performed on 3 patients, as stated above. Then, the expression of 141 genes involved in fibrosis was assessed using an Affymetrix HG_U133A microarray. The similarity of the patients’ transcriptomes was compared using hierarchical clusterisation.
Results: Hierarchical clusterisation demonstrated that the profile of gene expression in post-infarction myocardium was different than that in the remaining specimens. Regression analysis showed that in post-infraction sample genes encoding caspase 4, PDCD8, BBC3, BIT1 and DIABLO were overexpressed, whereas transcriptional activity of 2 genes encoding TNFα receptors presented a lower expression in the post-infarction sample.
Conclusions: The profile of apoptosis-associated genes is significantly different in post-myocardial infarction (post-MI) myocardium compared to chronically ischemic and non-ischemic myocardium. Our data suggest the importance of proapoptotic factors associated with mitochondria. Genes encoding a cascade of caspases were undifferentiating, while 2 genes encoding TNFa receptors were underexpressed in post-infarction tissue.
Get Citation

Keywords

oligonucleotide microarray; apoptosis; myocardial infarction

About this article
Title

Expression profiles of genes associated with apoptosis assessed by oligonucleotide microarray analysis in advanced coronary artery disease and post-infarction patients

Journal

Chirurgia Polska (Polish Surgery)

Issue

Vol 8, No 3 (2006)

Pages

184-190

Published online

2006-08-02

Bibliographic record

Chirurgia Polska 2006;8(3):184-190.

Keywords

oligonucleotide microarray
apoptosis
myocardial infarction

Authors

Józefa Dąbek
Zbigniew Gąsior
Przemysław Szmagała
Aleksander Owczarek
Andrzej Kułach
Barbara Monastyrska-Cup
Urszula Mazurek
Andrzej Bochenek

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

Via MedicaBy "Via Medica sp. z o.o." sp.k., ul. Świętokrzyska 73, 80–180 Gdańsk

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail: viamedica@viamedica.pl