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Visualization of blood vessels by means of the modified corrosion method
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Abstract
Material and methods: Investigations were carried out on adult male Sprague-Dawley rats. The agreement of Local Ethical Committee was obtained. After deep anaesthesia the chest organs were taken out. The heart was perfused using Langendorff’s method, (Krebs-Henseleit solution) for one minute and latex (LKBS 45–50) was administered for 30 min. under 15 cm H2O pressure. Then the specimen was fixed in a Bouin solution for 4 days. Afterwards the specimen was digested in concentrated HCl for 24 hours followed by digestion in a 25% solution of HCl. Before the corrosion had started, as well as during the process of corrosion which lasted 4 days, the heart was observed under the stereomicroscope every few hours, outlines were made and photographs taken. This digestion was interrupted when small vascular branches were visualized with the heart’s ventricle walls still present.
Results: The main stems of both the left and right coronary arteries and their branches including very small ones, became visible. It was easy to observe relations of the vessels to the organ wall, as wall as the resence of connections between arteries Conclusion: Our modified corrosive method enables direct observation even of the small vascular twigs, with the organ wall preserved.
Abstract
Material and methods: Investigations were carried out on adult male Sprague-Dawley rats. The agreement of Local Ethical Committee was obtained. After deep anaesthesia the chest organs were taken out. The heart was perfused using Langendorff’s method, (Krebs-Henseleit solution) for one minute and latex (LKBS 45–50) was administered for 30 min. under 15 cm H2O pressure. Then the specimen was fixed in a Bouin solution for 4 days. Afterwards the specimen was digested in concentrated HCl for 24 hours followed by digestion in a 25% solution of HCl. Before the corrosion had started, as well as during the process of corrosion which lasted 4 days, the heart was observed under the stereomicroscope every few hours, outlines were made and photographs taken. This digestion was interrupted when small vascular branches were visualized with the heart’s ventricle walls still present.
Results: The main stems of both the left and right coronary arteries and their branches including very small ones, became visible. It was easy to observe relations of the vessels to the organ wall, as wall as the resence of connections between arteries Conclusion: Our modified corrosive method enables direct observation even of the small vascular twigs, with the organ wall preserved.
Keywords
Sprague-Dawley rats; arteries; modified gradual corrosion method
About this articleTitle
Visualization of blood vessels by means of the modified corrosion method
Journal
Chirurgia Polska (Polish Surgery)
Issue
Pages
179-183
Published online
2006-08-02
Page views
643
Article views/downloads
2274
DOI
10.5603/chp.28891
Bibliographic record
Chirurgia Polska 2006;8(3):179-183.
Keywords
Sprague-Dawley rats
arteries
modified gradual corrosion method
Authors
Konstanty Ślusarczyk
Stanisław Skrzelewski
Jerzy Nożyński
Agnieszka Kędzia
Maurycy Porc
Brygida Beck-Ciszek
Tadeusz F. Krzemiński
