open access

Vol 8, No 3 (2006)
Published online: 2006-08-02
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Biofilm as the basic mechanism of surgical site infection - prevention methods in local treatment

Marzenna Bartoszewicz, Anna Rygiel
Chirurgia Polska 2006;8(3):171-178.

open access

Vol 8, No 3 (2006)
Published online: 2006-08-02

Abstract

Background: Surgical site infections are a common cause of post-operative complications. Particular attention should be paid to infections occurring in patients who have had implants, i.e. valves, vascular and bone implants, joint prostheses, or tendon prostheses made of biomaterials. These infections are usually a result of biomaterial contamination occurring at the moment of its implantation or of a transient bacteraemia. The purpose of our work was an assessment of the impact of Octenisept (disinfectant liquid) on the survival rate of bacteria producing biofilm on biomaterials.
Material and methods: In order to estimate the biofilm production ratio among 25 examined strains, a silicon-coated latex catheter was used along with a non-absorbable polyamide surgical thread, 1% TTC solution (2, 3, 5-triphenyltetrazolium chloride), Octenisept 0.5% saponin working solution, neutralising agent composed of: 3% Tween 80, 3% saponin, 0.1% histidine, 0.1% lecithin.
Results: All tested bacteria generated mucus on the catheter and surgical thread. In the method employing TTC, 100% of the strains were removed from the surgical thread after a 1-minute application of Octenisept. Using the same method 99% of the strains producing biofilm were removed from a urinary catheter after a 1-minute application of Octenisept. The number of living cells in the biofilm of all examined strains, initially being at a level of 107 CFU/ml and 109 CFU/ml, was reduced to 102 CFU/ml within the 1-minute long action of applying the solution. The action of Octenisept antiseptic preparation, which is composed of octenidyne dihydrochloride, phenoxymethyl alcohol and auxiliary substances, was, under examination conditions, similar in regards to all tested species of biofilm-producing bacteria.
Conclusions: Octenisept antiseptic preparation may be safely applied to disinfect skin, mucous membranes and wounds in areas infected by biofilm-producing bacteria.

Abstract

Background: Surgical site infections are a common cause of post-operative complications. Particular attention should be paid to infections occurring in patients who have had implants, i.e. valves, vascular and bone implants, joint prostheses, or tendon prostheses made of biomaterials. These infections are usually a result of biomaterial contamination occurring at the moment of its implantation or of a transient bacteraemia. The purpose of our work was an assessment of the impact of Octenisept (disinfectant liquid) on the survival rate of bacteria producing biofilm on biomaterials.
Material and methods: In order to estimate the biofilm production ratio among 25 examined strains, a silicon-coated latex catheter was used along with a non-absorbable polyamide surgical thread, 1% TTC solution (2, 3, 5-triphenyltetrazolium chloride), Octenisept 0.5% saponin working solution, neutralising agent composed of: 3% Tween 80, 3% saponin, 0.1% histidine, 0.1% lecithin.
Results: All tested bacteria generated mucus on the catheter and surgical thread. In the method employing TTC, 100% of the strains were removed from the surgical thread after a 1-minute application of Octenisept. Using the same method 99% of the strains producing biofilm were removed from a urinary catheter after a 1-minute application of Octenisept. The number of living cells in the biofilm of all examined strains, initially being at a level of 107 CFU/ml and 109 CFU/ml, was reduced to 102 CFU/ml within the 1-minute long action of applying the solution. The action of Octenisept antiseptic preparation, which is composed of octenidyne dihydrochloride, phenoxymethyl alcohol and auxiliary substances, was, under examination conditions, similar in regards to all tested species of biofilm-producing bacteria.
Conclusions: Octenisept antiseptic preparation may be safely applied to disinfect skin, mucous membranes and wounds in areas infected by biofilm-producing bacteria.
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Keywords

infection; biofilm; prevention

About this article
Title

Biofilm as the basic mechanism of surgical site infection - prevention methods in local treatment

Journal

Chirurgia Polska (Polish Surgery)

Issue

Vol 8, No 3 (2006)

Pages

171-178

Published online

2006-08-02

Bibliographic record

Chirurgia Polska 2006;8(3):171-178.

Keywords

infection
biofilm
prevention

Authors

Marzenna Bartoszewicz
Anna Rygiel

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