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The activity of monoamine oxidase in nervous tissue during mechanical jaundice in the rat model
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Abstract
Material and methods: The male albino Wistar rats was used in this experiment. The rats were divided into three groups of twenty one rats. Group 1 included sham operated animals which served as the control, Group 2 consisted of rats with permanent jaundice and Group 3 — those with temporary mechanical jaundice. These groups were divided into 3 subgroups of seven rats: Groups A — examined 2 weeks after surgery, Groups B — 4 weeks, and Groups C — 6 weeks after surgery. From the blood samples serum levels of total bilirubin and alkaline phosphatase activity were obtained. In the homogenate of the nervous tissue (cerebral, cerebellum and brain stem) the activity of MAO was measured.
Results: MAO activity increased during cholestasis in nervous tissue. After recanalization, activity reduction in the group with temporary jaundice was observed.
Conclusions: Intervention to repair the cause of jaundice is of paramount importance and should not be delayed. The animal model described in our study is suitable for mechanical jaundice examination.
Abstract
Material and methods: The male albino Wistar rats was used in this experiment. The rats were divided into three groups of twenty one rats. Group 1 included sham operated animals which served as the control, Group 2 consisted of rats with permanent jaundice and Group 3 — those with temporary mechanical jaundice. These groups were divided into 3 subgroups of seven rats: Groups A — examined 2 weeks after surgery, Groups B — 4 weeks, and Groups C — 6 weeks after surgery. From the blood samples serum levels of total bilirubin and alkaline phosphatase activity were obtained. In the homogenate of the nervous tissue (cerebral, cerebellum and brain stem) the activity of MAO was measured.
Results: MAO activity increased during cholestasis in nervous tissue. After recanalization, activity reduction in the group with temporary jaundice was observed.
Conclusions: Intervention to repair the cause of jaundice is of paramount importance and should not be delayed. The animal model described in our study is suitable for mechanical jaundice examination.
Keywords
mechanical jaundice; monoamine oxidase; nervous tissue
About this articleTitle
The activity of monoamine oxidase in nervous tissue during mechanical jaundice in the rat model
Journal
Chirurgia Polska (Polish Surgery)
Issue
Pages
237-242
Published online
2007-01-19
Bibliographic record
Chirurgia Polska 2006;8(4):237-242.
Keywords
mechanical jaundice
monoamine oxidase
nervous tissue
Authors
Brygida Beck
Barbara Królak-Olejnik
Jacek Karasiewicz
Konstanty Ślusarczyk
Wojciech Król
Iwona Rajca-Biernacka
