open access

Vol 8, No 4 (2006)
Published online: 2007-01-19
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The activity of monoamine oxidase in nervous tissue during mechanical jaundice in the rat model

Brygida Beck, Barbara Królak-Olejnik, Jacek Karasiewicz, Konstanty Ślusarczyk, Wojciech Król, Iwona Rajca-Biernacka
Chirurgia Polska 2006;8(4):237-242.

open access

Vol 8, No 4 (2006)
Published online: 2007-01-19

Abstract

Background: Autointoxication and hemodynamic disturbances play a leading role in the pathogenesis of neurological disorders in mechanical jaundice The mitochondrial flavoenzyme monoamine oxidase (MAO) [EC 1.4.3.4] is of special interest for neuropsychiatry. This investigation studied the activity of MAO in nervous tissue (cerebral, cerebellum and brain stem) during mechanical jaundice in rats.
Material and methods: The male albino Wistar rats was used in this experiment. The rats were divided into three groups of twenty one rats. Group 1 included sham operated animals which served as the control, Group 2 consisted of rats with permanent jaundice and Group 3 — those with temporary mechanical jaundice. These groups were divided into 3 subgroups of seven rats: Groups A — examined 2 weeks after surgery, Groups B — 4 weeks, and Groups C — 6 weeks after surgery. From the blood samples serum levels of total bilirubin and alkaline phosphatase activity were obtained. In the homogenate of the nervous tissue (cerebral, cerebellum and brain stem) the activity of MAO was measured.
Results: MAO activity increased during cholestasis in nervous tissue. After recanalization, activity reduction in the group with temporary jaundice was observed.
Conclusions: Intervention to repair the cause of jaundice is of paramount importance and should not be delayed. The animal model described in our study is suitable for mechanical jaundice examination.

Abstract

Background: Autointoxication and hemodynamic disturbances play a leading role in the pathogenesis of neurological disorders in mechanical jaundice The mitochondrial flavoenzyme monoamine oxidase (MAO) [EC 1.4.3.4] is of special interest for neuropsychiatry. This investigation studied the activity of MAO in nervous tissue (cerebral, cerebellum and brain stem) during mechanical jaundice in rats.
Material and methods: The male albino Wistar rats was used in this experiment. The rats were divided into three groups of twenty one rats. Group 1 included sham operated animals which served as the control, Group 2 consisted of rats with permanent jaundice and Group 3 — those with temporary mechanical jaundice. These groups were divided into 3 subgroups of seven rats: Groups A — examined 2 weeks after surgery, Groups B — 4 weeks, and Groups C — 6 weeks after surgery. From the blood samples serum levels of total bilirubin and alkaline phosphatase activity were obtained. In the homogenate of the nervous tissue (cerebral, cerebellum and brain stem) the activity of MAO was measured.
Results: MAO activity increased during cholestasis in nervous tissue. After recanalization, activity reduction in the group with temporary jaundice was observed.
Conclusions: Intervention to repair the cause of jaundice is of paramount importance and should not be delayed. The animal model described in our study is suitable for mechanical jaundice examination.
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Keywords

mechanical jaundice; monoamine oxidase; nervous tissue

About this article
Title

The activity of monoamine oxidase in nervous tissue during mechanical jaundice in the rat model

Journal

Chirurgia Polska (Polish Surgery)

Issue

Vol 8, No 4 (2006)

Pages

237-242

Published online

2007-01-19

Bibliographic record

Chirurgia Polska 2006;8(4):237-242.

Keywords

mechanical jaundice
monoamine oxidase
nervous tissue

Authors

Brygida Beck
Barbara Królak-Olejnik
Jacek Karasiewicz
Konstanty Ślusarczyk
Wojciech Król
Iwona Rajca-Biernacka

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