Vol 8, No 4 (2006)
Published online: 2007-01-19
The activity of monoamine oxidase in nervous tissue during mechanical jaundice in the rat model
Chirurgia Polska 2006;8(4):237-242.
Abstract
Background: Autointoxication and hemodynamic disturbances play a leading role in the pathogenesis of
neurological disorders in mechanical jaundice The mitochondrial flavoenzyme monoamine oxidase (MAO)
[EC 1.4.3.4] is of special interest for neuropsychiatry. This investigation studied the activity of MAO in
nervous tissue (cerebral, cerebellum and brain stem) during mechanical jaundice in rats.
Material and methods: The male albino Wistar rats was used in this experiment. The rats were divided into three groups of twenty one rats. Group 1 included sham operated animals which served as the control, Group 2 consisted of rats with permanent jaundice and Group 3 — those with temporary mechanical jaundice. These groups were divided into 3 subgroups of seven rats: Groups A — examined 2 weeks after surgery, Groups B — 4 weeks, and Groups C — 6 weeks after surgery. From the blood samples serum levels of total bilirubin and alkaline phosphatase activity were obtained. In the homogenate of the nervous tissue (cerebral, cerebellum and brain stem) the activity of MAO was measured.
Results: MAO activity increased during cholestasis in nervous tissue. After recanalization, activity reduction in the group with temporary jaundice was observed.
Conclusions: Intervention to repair the cause of jaundice is of paramount importance and should not be delayed. The animal model described in our study is suitable for mechanical jaundice examination.
Material and methods: The male albino Wistar rats was used in this experiment. The rats were divided into three groups of twenty one rats. Group 1 included sham operated animals which served as the control, Group 2 consisted of rats with permanent jaundice and Group 3 — those with temporary mechanical jaundice. These groups were divided into 3 subgroups of seven rats: Groups A — examined 2 weeks after surgery, Groups B — 4 weeks, and Groups C — 6 weeks after surgery. From the blood samples serum levels of total bilirubin and alkaline phosphatase activity were obtained. In the homogenate of the nervous tissue (cerebral, cerebellum and brain stem) the activity of MAO was measured.
Results: MAO activity increased during cholestasis in nervous tissue. After recanalization, activity reduction in the group with temporary jaundice was observed.
Conclusions: Intervention to repair the cause of jaundice is of paramount importance and should not be delayed. The animal model described in our study is suitable for mechanical jaundice examination.
Keywords: mechanical jaundicemonoamine oxidasenervous tissue
