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Vol 9, No 1 (2007)
Published online: 2007-02-01

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The effect of Allopurinol on intestinal ischemia-reperfusion injury development - the new approach

Tomasz Dawiskiba, Artur Pupka, Jan Skóra, Dariusz Janczak, Stanisław Pawłowski, Zbigniew Krawczyk, Przemysław P. Szyber, Piotr Szyber
Chirurgia Polska 2007;9(1):25-33.

Abstract


Background: The aim of the study was to evaluate the effect of Allopurinol on intestinal ischemia-reperfusion injury and xanthine oxidase system.
Material and methods: 70 Buffalo rats were divided into 5 groups of 14. Group I was the control group. The animals of groups II and IV were subjected to 50 minutes of acute intestinal ischemia. The animals of groups III and V were subjected to 50 minutes of acute intestinal ischemia followed by 60 minutes of reperfusion. The rats of group IV and V were pretreated with Allopurinol for three days before surgery. Total intestinal ischemia was produced by clamping the mesenteric vessels. Mucosal changes underwent histopathological evaluation according to the scoring system (0-5) described by Chiu. Biochemical tests of xanthine oxidase activity were performed in supernatants of small intestine homogenates.
Results: The administration of Allopurinol significantly reduced mesenteric ischemia-reperfusion injury as well as intestinal xanthine oxidase activity. However, there was no significant difference in the xanthine oxidase activity between non-pretreated rats subjected to ischemic processes and the control group. It was also noted that beneficial action of Allopurinol began during the ischemic phase, before reperfusion.
Conclusions: It can be concluded that the protective effect of Allopurinol against intestinal ischemiareperfusion is not only related to the reperfusion component. The role of xanthine oxidase in the processes of injury resulting from blood flow disturbances remains a matter of controversy.

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