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The effect of Allopurinol on intestinal ischemia-reperfusion injury development - the new approach
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Abstract
Background: The aim of the study was to evaluate the effect of Allopurinol on intestinal ischemia-reperfusion injury and xanthine oxidase system.
Material and methods: 70 Buffalo rats were divided into 5 groups of 14. Group I was the control group. The animals of groups II and IV were subjected to 50 minutes of acute intestinal ischemia. The animals of groups III and V were subjected to 50 minutes of acute intestinal ischemia followed by 60 minutes of reperfusion. The rats of group IV and V were pretreated with Allopurinol for three days before surgery. Total intestinal ischemia was produced by clamping the mesenteric vessels. Mucosal changes underwent histopathological evaluation according to the scoring system (0-5) described by Chiu. Biochemical tests of xanthine oxidase activity were performed in supernatants of small intestine homogenates.
Results: The administration of Allopurinol significantly reduced mesenteric ischemia-reperfusion injury as well as intestinal xanthine oxidase activity. However, there was no significant difference in the xanthine oxidase activity between non-pretreated rats subjected to ischemic processes and the control group. It was also noted that beneficial action of Allopurinol began during the ischemic phase, before reperfusion.
Conclusions: It can be concluded that the protective effect of Allopurinol against intestinal ischemiareperfusion is not only related to the reperfusion component. The role of xanthine oxidase in the processes of injury resulting from blood flow disturbances remains a matter of controversy.
Abstract
Background: The aim of the study was to evaluate the effect of Allopurinol on intestinal ischemia-reperfusion injury and xanthine oxidase system.
Material and methods: 70 Buffalo rats were divided into 5 groups of 14. Group I was the control group. The animals of groups II and IV were subjected to 50 minutes of acute intestinal ischemia. The animals of groups III and V were subjected to 50 minutes of acute intestinal ischemia followed by 60 minutes of reperfusion. The rats of group IV and V were pretreated with Allopurinol for three days before surgery. Total intestinal ischemia was produced by clamping the mesenteric vessels. Mucosal changes underwent histopathological evaluation according to the scoring system (0-5) described by Chiu. Biochemical tests of xanthine oxidase activity were performed in supernatants of small intestine homogenates.
Results: The administration of Allopurinol significantly reduced mesenteric ischemia-reperfusion injury as well as intestinal xanthine oxidase activity. However, there was no significant difference in the xanthine oxidase activity between non-pretreated rats subjected to ischemic processes and the control group. It was also noted that beneficial action of Allopurinol began during the ischemic phase, before reperfusion.
Conclusions: It can be concluded that the protective effect of Allopurinol against intestinal ischemiareperfusion is not only related to the reperfusion component. The role of xanthine oxidase in the processes of injury resulting from blood flow disturbances remains a matter of controversy.
Keywords
intestinal ischemia-reperfusion injury; allopurinol; xanthine oxidase
About this articleTitle
The effect of Allopurinol on intestinal ischemia-reperfusion injury development - the new approach
Journal
Chirurgia Polska (Polish Surgery)
Issue
Pages
25-33
Published online
2007-02-01
Page views
504
Article views/downloads
1179
Bibliographic record
Chirurgia Polska 2007;9(1):25-33.
Keywords
intestinal ischemia-reperfusion injury
allopurinol
xanthine oxidase
Authors
Tomasz Dawiskiba
Artur Pupka
Jan Skóra
Dariusz Janczak
Stanisław Pawłowski
Zbigniew Krawczyk
Przemysław P. Szyber
Piotr Szyber
