Vol 9, No 3 (2007)
Published online: 2008-01-04
Gene expression profiles in abdominal aortic aneurysm or aortoiliac occlusive disease assessed by cDNA Atlas arrays
Chirurgia Polska 2007;9(3):149-161.
Abstract
Background: Abdominal aortic aneurysms and aortoiliac occlusive disease are the most frequent reasons
for vascular surgery procedures within the abdominal cavity. Genetic factors responsible for individual
risk of the development of these diseases are little known. The purpose of the present study was to
attempt to compare abdominal aortic aneurysm and aortoiliac occlusive disease using the analysis of
gene expression profiles with Atlas cDNA arrays.
Material and methods: Specimens of abdominal aorta from 12 patients with AAA, 8 with AIOD and 5 organ donors were used. Total RNA was isolated from aorta tissue and subjected to reverse transcription, then hybridized to Atlas cDNA arrays.
Results: We identified 36 genes (20 from the Atlas Cardiovascular Array and 16 from the Atlas Stress Array) which were significantly differentially expressed in AAA and AIOD.
Conclusions: The results of gene expression profiling in AAA and AIOD indicate the essential role of the genes responsible for inflammation process and extracellular matrix remodelling in both diseases.
Material and methods: Specimens of abdominal aorta from 12 patients with AAA, 8 with AIOD and 5 organ donors were used. Total RNA was isolated from aorta tissue and subjected to reverse transcription, then hybridized to Atlas cDNA arrays.
Results: We identified 36 genes (20 from the Atlas Cardiovascular Array and 16 from the Atlas Stress Array) which were significantly differentially expressed in AAA and AIOD.
Conclusions: The results of gene expression profiling in AAA and AIOD indicate the essential role of the genes responsible for inflammation process and extracellular matrix remodelling in both diseases.
Keywords: abdominal aortic aneurysmaortoiliac occlusive diseasegene expression profilesAtlas cDNA arrays
