open access

Vol 12, No 1 (2010)
Published online: 2010-08-13
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The content of metallic and non-metallic elements in homogenates of tissue samples collected from the lower limbs amputated on the level of thigh due to atherosclerosis. Preliminary report

Damian Ziaja, Ewa John, Barbara Mikuła i wsp.
Chirurgia Polska 2010;12(1):26-32.

open access

Vol 12, No 1 (2010)
Published online: 2010-08-13

Abstract

Background: Metallic and non-metallic elements have influence on the development the changes within structure and function of arteries in different regions of vascular tree. The content of elements into arterial walls varies according to geographical origin of people.
Material and methods: Tissue specimens were taken from the six lower limbs amputated on the level of thigh due to atherosclerosis. The mean age was 69 (57 to 84). Tissue specimens were collected from the limbs from the level of amputation, the popliteal fossa and the dorsal artery of foot. After chemical processing of the tissues homogenates determination of the content of elements using atomic emission spectrometry method with inductively coupled plasma (inductively coupled plasma optical emission spectroscopy) was performed. The content of Al, Ba, Ca, Cu, Fe, K, Mg, Mn, P, S, Sr and Zn was determined. Results: As a point of reference mean absolute value occurred from six specimens on the level of thigh was assumed as 100%. Content of non-metallic elements such as Ca, P and S rise towards to periphery of amputated limb reaching few times higher values on the level of dorsal artery of foot. Content of some metallic elements such as: Fe, Zn, K and Sr also rise towards to periphery of extremity. The Cu accumulation was the highest on the level of popliteal fossa, while Mn, Al and Mg concentration was the lowest on this same level and the highest in dorsal artery of foot.
Conclusions: The influence of individual elements on development the atherosclerosis especially on necrotic changes requires further investigations. Described problem pose a preliminary report only in order to indicate the issue and to check the method of analysis.
Polish Surgery 2010, 12, 1, 26-32

Abstract

Background: Metallic and non-metallic elements have influence on the development the changes within structure and function of arteries in different regions of vascular tree. The content of elements into arterial walls varies according to geographical origin of people.
Material and methods: Tissue specimens were taken from the six lower limbs amputated on the level of thigh due to atherosclerosis. The mean age was 69 (57 to 84). Tissue specimens were collected from the limbs from the level of amputation, the popliteal fossa and the dorsal artery of foot. After chemical processing of the tissues homogenates determination of the content of elements using atomic emission spectrometry method with inductively coupled plasma (inductively coupled plasma optical emission spectroscopy) was performed. The content of Al, Ba, Ca, Cu, Fe, K, Mg, Mn, P, S, Sr and Zn was determined. Results: As a point of reference mean absolute value occurred from six specimens on the level of thigh was assumed as 100%. Content of non-metallic elements such as Ca, P and S rise towards to periphery of amputated limb reaching few times higher values on the level of dorsal artery of foot. Content of some metallic elements such as: Fe, Zn, K and Sr also rise towards to periphery of extremity. The Cu accumulation was the highest on the level of popliteal fossa, while Mn, Al and Mg concentration was the lowest on this same level and the highest in dorsal artery of foot.
Conclusions: The influence of individual elements on development the atherosclerosis especially on necrotic changes requires further investigations. Described problem pose a preliminary report only in order to indicate the issue and to check the method of analysis.
Polish Surgery 2010, 12, 1, 26-32
Get Citation

Keywords

metallic and non-metallic elements; atherosclerosis; peripheral artery disease

About this article
Title

The content of metallic and non-metallic elements in homogenates of tissue samples collected from the lower limbs amputated on the level of thigh due to atherosclerosis. Preliminary report

Journal

Chirurgia Polska (Polish Surgery)

Issue

Vol 12, No 1 (2010)

Pages

26-32

Published online

2010-08-13

Bibliographic record

Chirurgia Polska 2010;12(1):26-32.

Keywords

metallic and non-metallic elements
atherosclerosis
peripheral artery disease

Authors

Damian Ziaja
Ewa John
Barbara Mikuła i wsp.

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