open access

Vol 13, No 1 (2011)
Published online: 2011-10-19
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Liver transplantation in animal model with transgenic pig’s liver

Krzysztof Ziaja, Tomasz Urbanek, Wacław Kuczmik, Marcin Kucharzewski, Grzegorz Biolik, Damian Ziaja, Dariusz Stańczyk, Arkadiusz Krupowies, Jacek Kostyra, Robert Latała, Tomasz Ludyga, Marek Kazibudzki, Jerzy Stojko, Barbara Sidorowicz, Henryka Chmurzewska
Chirurgia Polska 2011;13(1):28-41.

open access

Vol 13, No 1 (2011)
Published online: 2011-10-19

Abstract

Introduction: In this paper our own experience concerning the animal model of orthotopic liver transplantation is presented.
Material and methods: Twenty seven liver transplantations (pig-to-pig) were performed, including 9 cases of transgenic animal transplantation. In the transgenesis, the human α-(1, 2)-fucosyltransferase gene was implemented. After transplantation the clinical conditions of the transplanted animals were followed and pathological studies of the transplanted livers were conducted. To prove the influence of the performed transgenesis, additionally, the human blood extracorporeal perfusion model of the transgenic animal liver was also proposed.
Results: According to the study’s results, the implementation of transgenesis did not result in immunological reaction changes in the pig to pig animal model. However, a limited influence on xenoantibodyrelated rejection reaction in the pig liver perfusion model was observed.
Conclusions: Thus, the results highlight the necessity of further studies as currently, there are no data confirming the possibility to use such transgenic animal organs for liver xenotransplantation.
Polish Surgery 2011, 13, 1, 28–41

Abstract

Introduction: In this paper our own experience concerning the animal model of orthotopic liver transplantation is presented.
Material and methods: Twenty seven liver transplantations (pig-to-pig) were performed, including 9 cases of transgenic animal transplantation. In the transgenesis, the human α-(1, 2)-fucosyltransferase gene was implemented. After transplantation the clinical conditions of the transplanted animals were followed and pathological studies of the transplanted livers were conducted. To prove the influence of the performed transgenesis, additionally, the human blood extracorporeal perfusion model of the transgenic animal liver was also proposed.
Results: According to the study’s results, the implementation of transgenesis did not result in immunological reaction changes in the pig to pig animal model. However, a limited influence on xenoantibodyrelated rejection reaction in the pig liver perfusion model was observed.
Conclusions: Thus, the results highlight the necessity of further studies as currently, there are no data confirming the possibility to use such transgenic animal organs for liver xenotransplantation.
Polish Surgery 2011, 13, 1, 28–41
Get Citation

Keywords

liver transplantation; transgenesis; xenotransplantation

About this article
Title

Liver transplantation in animal model with transgenic pig’s liver

Journal

Chirurgia Polska (Polish Surgery)

Issue

Vol 13, No 1 (2011)

Pages

28-41

Published online

2011-10-19

Bibliographic record

Chirurgia Polska 2011;13(1):28-41.

Keywords

liver transplantation
transgenesis
xenotransplantation

Authors

Krzysztof Ziaja
Tomasz Urbanek
Wacław Kuczmik
Marcin Kucharzewski
Grzegorz Biolik
Damian Ziaja
Dariusz Stańczyk
Arkadiusz Krupowies
Jacek Kostyra
Robert Latała
Tomasz Ludyga
Marek Kazibudzki
Jerzy Stojko
Barbara Sidorowicz
Henryka Chmurzewska

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