Vol 27, No 2 (2022)
Research paper
Published online: 2022-03-28

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Expression of microRNAs miR-21 and miR-326 associated with HIF-1α regulation in neurospheres of glioblastoma submitted to ionizing radiation treatment

Letícia Passi Turra1, Andressa Romualdo Rodrigues1, Fermino Sanches Lizarte Neto1, Paulo Cezar Novais12, Maria Julia Nunes1, Victor Cunha Tirapelli1, Fernanda Maris Peria1, Vinícius Marques Carneiro1, Mucio Luiz de Assis Cirino13, Carlos Gilberto Carlotti Jr1, Daniela Pretti da Cunha Tirapelli1
Rep Pract Oncol Radiother 2022;27(2):215-225.


Background: Glioblastoma is an incurable neoplasm. Its hypoxia mechanism associated with cancer stem cells (CSCs) demonstrates hypoxia-inducible factor 1α (HIF-1α) expression regulation, which is directly related to tumor malignancy.

The aim of this study was to identify a possible tumor malignancy signature associated with regulation of HIF-1α by microRNAs miR-21 and miR-326 in the subpopulation of tumor stem cells which were irradiated  by ion in primary culture of patients diagnosed with glioblastoma.

Materials and methods: We used cellular cultures from surgery biopsies of ten patients with glioblastoma. MicroRNA expressions were analyzed through real-time polymerase chain reaction (PCR) and correlated with mortality and recurrence. The ROC curve displayed the cutoff point of the respective microRNAs in relation to the clinical prognosis, separating them by group.

Results: The miR-21 addressed high level of expression in the irradiated neurosphere group (p = 0.0028). However, miR-21 was not associated with recurrence and mortality. miR-326 can be associated with tumoral recurrence (p = 0.032) in both groups; every 0.5 units of miR-326 increased the chances of recurrence by 1,024 (2.4%).

Conclusion: The high expression of miR-21 in the irradiated group suggests its role in the regulation of HIF-1α and in the radioresistant neurospheres. miR-326 increased the chances of recurrence in both groups, also demonstrating that positive regulation from miR-326 does not depend on ionizing radiation treatment.

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