Vol 26, No 5 (2021)
Research paper
Published online: 2021-08-13
Radiosensitization effects by bismuth oxide nanorods of different sizes in megavoltage external beam radiotherapy
Rep Pract Oncol Radiother 2021;26(5):773-784.
Abstract
Background: Nanotechnology application has successfully reached numerous scientific breakthroughs including in radiotherapy. However, the clinical application of nanoparticles requires more diligent research primarily on the crucial parameters such as nanoparticle sizes.
This study is aimed to investigate the influence of bismuth oxide nanorod (Bi2O3-NR) sizes on radiosensitization effects on MCF-7 and HeLa cell lines for megavoltage photon and electron beam radiotherapy.
Materials and methods: MCF-7 and HeLa cells were treated with and without 0.5 µMol/L of Bi2O3-NR of varying sizes (60, 70, 80, and 90 nm). The samples, including the control groups, were exposed to different radiation doses (0–10 Gy), using photon (6 MV and 10 MV), and electron beam (6 MeV and 12 MeV) radiotherapy. Clonogenic assay was performed, and sensitization enhancement ratio (SER) was determined from linear quadratic based cell survival curves.
Results: The results depicted that 60 nm Bi2O3-NR yields the most excellent SER followed by 70 nm Bi2O3-NR. Meanwhile, the 80 and 90 nm Bi2O3-NR showed an insignificant difference between treated and untreated cell groups. This study also found that MCF-7 was subjected to more cell death compared to HeLa.
Conclusion: 60 nm Bi2O3-NR was the optimal Bi2O3-NR size to induce radiosensitization effects for megavoltage external beam radiotherapy. The SER in photon beam radiotherapy marked the highest compared to electron beam radiotherapy due to decreased primary radiation energy from multiple radiation interaction and higher Compton scattering.
Keywords: bismuth oxide nanorodscancer cell lineselectron beamnanoparticlesphoton beamradiosensitization
References
- Boateng F, Ngwa W. Delivery of Nanoparticle-Based Radiosensitizers for Radiotherapy Applications. Int J Mol Sci. 2019; 21(1).
- Ngwa W, Kumar R, Moreau M, et al. Nanoparticle Drones to Target Lung Cancer with Radiosensitizers and Cannabinoids. Front Oncol. 2017; 7: 208.
- Boateng F. In Silico Study of Smart Radiotherapy Biomaterials for Radiotherapy Alications. Vol. 10675302. ProQuest Dissertations Publishing, Lowell 2017: 1–118.
- Pottier A, Borghi E, Levy L. The future of nanosized radiation enhancers. Br J Radiol. 2015; 88(1054): 20150171.
- Kwatra D, Venugopal A, Anant S. Nanoparticles in radiation therapy: A summary of various approaches to enhance radiosensitization in cancer. Transl Cancer Res. 2013; 2(4): 330–342.
- Zhang P, Qiao Y, Xia J, et al. Enhanced radiation therapy with multilayer microdisks containing radiosensitizing gold nanoparticles. ACS Appl Mater Interfaces. 2015; 7(8): 4518–4524.
- Paro AD, Shanmugam I, van de Ven AL. Nanoparticle-Mediated X-Ray Radiation Enhancement for Cancer Therapy. Methods Mol Biol. 2017; 1530: 391–401.
- Atun R, Jaffray DA, Barton MB, et al. Expanding global access to radiotherapy. Lancet Oncol. 2015; 16(10): 1153–1186.
- Kvols LK. Radiation sensitizers: a selective review of molecules targeting DNA and non-DNA targets. J Nucl Med. 2005; 46 Suppl 1: 187S–90S.
- Tang Le, Wei F, Wu Y, et al. Role of metabolism in cancer cell radioresistance and radiosensitization methods. J Exp Clin Cancer Res. 2018; 37(1): 87.
- Kwatra D, Venugopal A, Anant S. Nanoparticles in radiation therapy: A summary of various approaches to enhance radiosensitization in cancer. Transl Cancer Res. 2013; 2(4): 330–342.
- Zhang P, Qiao Y, Xia J, et al. Enhanced radiation therapy with multilayer microdisks containing radiosensitizing gold nanoparticles. ACS Appl Mater Interfaces. 2015; 7(8): 4518–4524.
- Paro AD, Shanmugam I, van de Ven AL. Nanoparticle-Mediated X-Ray Radiation Enhancement for Cancer Therapy. Methods Mol Biol. 2017; 1530: 391–401.
- Nagi NMS, Khair YAM, Abdalla AME. Capacity of gold nanoparticles in cancer radiotherapy. Jpn J Radiol. 2017; 35(10): 555–561.
- Cheng YJ, Nie XY, Ji CC, et al. Long-Term Cardiovascular Risk After Radiotherapy in Women With Breast Cancer. J Am Heart Assoc. 2017; 6(5).
- Nimmagadda S, Ford EC, Wong JW, et al. Targeted molecular imaging in oncology: focus on radiation therapy. Semin Radiat Oncol. 2008; 18(2): 136–148.
- Willers H, Azzoli CG, Santivasi WL, et al. Basic mechanisms of therapeutic resistance to radiation and chemotherapy in lung cancer. Cancer J. 2013; 19(3): 200–207.
- Boateng F, Ngwa W. Modeling gold nanoparticle-eluting spacer degradation during brachytherapy application with in situ dose painting. Br J Radiol. 2017; 90(1074): 20170069.
- Farokhzad OC, Langer R. Impact of nanotechnology on drug delivery. ACS Nano. 2009; 3(1): 16–20.
- Wang H, Mu X, He H, et al. Cancer Radiosensitizers. Trends Pharmacol Sci. 2018; 39(1): 24–48.
- Rashid RA, Abidin SZ, Anuar MK, et al. Radiosensitization effects and ROS generation by high Z metallic nanoparticles on human colon carcinoma cell (HCT116) irradiated under 150 MeV proton beam. OpenNano. 2019; 4: 100027.
- Rashid R, Razak K, Geso M, et al. Radiobiological Characterization of the Radiosensitization Effects by Gold Nanoparticles for Megavoltage Clinical Radiotherapy Beams. BioNanoScience. 2018; 8(3): 713–722.
- K.A. M, Rashid R, Lazim R, et al. Evaluation of radiosensitization effects by platinum nanodendrites for 6 MV photon beam radiotherapy. Radiation Physics and Chemistry. 2018; 150: 40–45.
- Rahman WN. Gold nanoparticles: novel radiobiological dose enhancement studies for radiation therapy, synchrotron based microbeam and stereotactic radiotherapy. RMIT University, Melbourne 2010.
- Xiong Y, Wu M, Ye J, et al. Synthesis and luminescence properties of hand-like α-Bi2O3 microcrystals. Materials Letters. 2008; 62(8-9): 1165–1168.
- Yang Q, Li Y, Yin Q, et al. Bi4Ti3O12 nanoparticles prepared by hydrothermal synthesis. J Eur Ceram Soc. 2003; 23(1): 161–166.
- Jeon S, Park S, Yu J, et al. Selective Synthesis of Nanospheres and Nanosheets of Bismuth Subcarbonate. Chemistry Lett. 2015; 44(12): 1717–1719.
- Yang H, Yan Y, Li J, et al. Low-temperature Hydrothermal Synthesis of Bismuth Nanoflowers and Their Application for Heavy Metal Detection. Chemistry Lett. 2013; 42(2): 150–152.
- Helal A, Harraz F, Ismail A, et al. Controlled synthesis of bismuth sulfide nanorods by hydrothermal method and their photocatalytic activity. Materials & Design. 2016; 102: 202–212.
- KIM H, LEE J, SHIM S. Study of Bi2O3 nanorods grown using the MOCVD technique. Sensors and Actuators B: Chemical. 2007; 126(1): 306–310.
- Li L, Yang YW, Li GH, et al. Conversion of a Bi nanowire array to an array of Bi-Bi2O3 core-shell nanowires and Bi2O3 nanotubes. Small. 2006; 2(4): 548–553.
- Li J, Liu X, Cui J, et al. Hydrothermal synthesis of self-assembled hierarchical tungsten oxides hollow spheres and their gas sensing properties. ACS Appl Mater Interfaces. 2015; 7(19): 10108–10114.
- Xu G, Ren Z, Du P, et al. Polymer-Assisted Hydrothermal Synthesis of Single-Crystalline Tetragonal Perovskite PbZr0.52Ti0.48O3 Nanowires. Advanced Materials. 2005; 17(7): 907–910.
- Byrappa K, Adschiri T. Hydrothermal technology for nanotechnology. Prog Crystal Growth Charact Mat. 2007; 53(2): 117–166.
- Muruganandham M, Amutha R, Lee GJ, et al. Facile Fabrication of Tunable Bi2O3 Self-Assembly and Its Visible Light Photocatalytic Activity. J Phys Chem C. 2012; 116(23): 12906–12915.
- Wu C, Shen Li, Huang Q, et al. Hydrothermal synthesis and characterization of Bi2O3 nanowires. Materials Lett. 2011; 65(7): 1134–1136.
- Yang Q, Li Y, Yin Q, et al. Hydrothermal synthesis of bismuth oxide needles. Materials Lett. 2002; 55(1-2): 46–49.
- Chen W, Zhang J. Using nanoparticles to enable simultaneous radiation and photodynamic therapies for cancer treatment. J Nanosci Nanotechnol. 2006; 6(4): 1159–1166.
- Bharti DB, Bharati AV. Synthesis of ZnO nanoparticles using a hydrothermal method and a study its optical activity. Luminescence. 2017; 32(3): 317–320.
- Aneesh PM, Vanaja KA, Jayaraj MK. Synthesis of ZnO nanoparticles by hydrothermal method. Nanophotonic Materials IV. 2007.
- Pan Yu, Neuss S, Leifert A, et al. Size-dependent cytotoxicity of gold nanoparticles. Small. 2007; 3(11): 1941–1949.
- Huang K, Ma H, Liu J, et al. Size-dependent localization and penetration of ultrasmall gold nanoparticles in cancer cells, multicellular spheroids, and tumors in vivo. ACS Nano. 2012; 6(5): 4483–4493.
- Ngwa W, Makrigiorgos GM, Berbeco RI. Applying gold nanoparticles as tumor-vascular disrupting agents during brachytherapy: estimation of endothelial dose enhancement. Phys Med Biol. 2010; 55(21): 6533–6548.
- Irmawati R, Nasriah MNN, Taufiq-Yap YH, et al. Characterization of bismuth oxide catalysts prepared from bismuth trinitrate pentahydrate: influence of bismuth concentration. Catalysis Today. 2004; 93-95: 701–709.
- Lechtman E, Pignol JP. Interplay between the gold nanoparticle sub-cellular localization, size, and the photon energy for radiosensitization. Sci Rep. 2017; 7(1): 13268.
- Hainfeld JF, Slatkin DN, Smilowitz HM. The use of gold nanoparticles to enhance radiotherapy in mice. Phys Med Biol. 2004; 49(18): N309–N315.
- Pan Yu, Neuss S, Leifert A, et al. Size-dependent cytotoxicity of gold nanoparticles. Small. 2007; 3(11): 1941–1949.
- Brun E, Sanche L, Sicard-Roselli C. Parameters governing gold nanoparticle X-ray radiosensitization of DNA in solution. Colloids Surf B Biointerfaces. 2009; 72(1): 128–134.
- Chithrani DB, Jelveh S, Jalali F, et al. Gold nanoparticles as radiation sensitizers in cancer therapy. Radiat Res. 2010; 173(6): 719–728.
- Stewart CA. An Investigation into the Tailoring of Bismuth Oxide Nanoceramic with a Biomedical Application as a High Z Radiation Enhancer for Cancer Therapy. University of Wollongong, Wollongong 2014.
- McMahon SJ, Paganetti H, Prise KM. Optimising element choice for nanoparticle radiosensitisers. Nanoscale. 2016; 8(1): 581–589.
- Khoshgard K, Kiani P, Haghparast A, et al. Radiation dose rate affects the radiosensitization of MCF-7 and HeLa cell lines to X-rays induced by dextran-coated iron oxide nanoparticles. Int J Radiat Biol. 2017; 93(8): 757–763.
- Anoopkumar-Dukie S, Conere T, Sisk GD, et al. Mitochondrial modulation of oxygen-dependent radiosensitivity in some human tumour cell lines. Br J Radiol. 2009; 82(982): 847–854.
- Ngwa W, Makrigiorgos GM, Berbeco RI. Gold nanoparticle-aided brachytherapy with vascular dose painting: estimation of dose enhancement to the tumor endothelial cell nucleus. Med Phys. 2012; 39(1): 392–398.
- Morozov KV, Kolyvanova MA, Kartseva ME, et al. Radiosensitization by Gold Nanoparticles: Impact of the Size, Dose Rate, and Photon Energy. Nanomaterials (Basel). 2020; 10(5).
- Jeremic B, Aguerri AR, Filipovic N. Radiosensitization by gold nanoparticles. Clin Transl Oncol. 2013; 15(8): 593–601.
- Feynman RP, Vernon FL. The theory of a general quantum system interacting with a linear dissipative system. Ann Phys. 1963; 24: 118–173.
- Talik Sisin NN, Abdul Razak K, Zainal Abidin S, et al. Synergetic Influence of Bismuth Oxide Nanoparticles, Cisplatin and Baicalein-Rich Fraction on Reactive Oxygen Species Generation and Radiosensitization Effects for Clinical Radiotherapy Beams. Int J Nanomedicine. 2020; 15: 7805–7823.