Vol 6 (2021): Continuous Publishing
Original paper
Published online: 2021-02-24
Modeling of bacterial keratitis of Pseudomonas aeruginosa etiology in the experimental model
1
, 1, 2
, 1, 2
DOI: 10.5603/OJ.2021.0007
Ophthalmol J 2021;6:17-22.
Abstract
Background: Pseudomonas aeruginosa keratitis demonstrates an aggressive course, high resistance to antimicrobial therapy, and it also leads to a significant reduction of visual acuity. The purpose of our study was to develop an experimental model of Pseudomonas aeruginosa keratitis and investigate clinical features of corneal lesions in rabbits.
Material and methods: A total of 32 rabbits were divided randomly into three groups. The animals of the experimental group 1 (8 rabbits) underwent scarification of the cornea and instillation of archival strain suspension of P. aeruginosa into the conjunctival sac. In group 2 (12 rabbits), corneal de-epithelialization followed by instillation of microbial suspension was performed. In animals of group 3 (12 rabbits), after the de-epithelialization followed by instillation of one-day suspension of the pathogen, the cornea’s surface was covered with a sterile soft contact lens for 16–24 hours. In half of the animals (6 rabbits), the contact lenses with bacterial films of P. aeruginosa were used as a modification.
Results: The technique used in group I resulted in bacterial conjunctivitis with mild corneal changes in all cases. In group II, keratitis development was noted in all animals, being defined as moderate in nine cases and semi-severe in three. In animals of group III, severe purulent keratitis occurred in 11 of 12 eyes and semi-severe — in one. Four cases of ulcers and two cases of corneal abscess were recorded, resulting in perforations and keratomalacia (2 cases each).
Conclusions: Modeling of purulent P. aeruginosa keratitis in rabbits of varying severity requires complete deepithelialization
cornea segment and prolonged presence of the pathogen on the affected surface. The effective way
to keep infectious organisms on the cornea surface in sufficient concentration is to use soft contact lenses.
Keywords: purulent keratitisPseudomonas aeruginosaexperimental modelrabbits
References
- Sereda K, Drozhzhyna G, Gaidamaka T. The effect of different techniques of cryopreserved amniotic membrane transplantation on the repair processes of the cornea on the bacterial keratitis model . Ophtalmology. East Europe. 2016; 6(2): 229–239.
- Paclyuchenko K, Mogilevskyy S, Tereshchenko Y, et al. Active approaches in the treatment of bacterial keratitis and purulent corneal ulcers. Arch Ukrain Ophthalmol. 2013; 1(1): 120–127.
- Drozhzhina GI, Ivanova ON, Ostashevsky VL, et al. Severe corneal infections, caused by contact lens wear. Ophthal J. 2016; 6: 38–43.
- Green M, Apel A, Stapleton F, et al. Risk factors and causative organisms in microbial keratitis. Cornea. 2008; 27(1): 22–27.
- Jin H, Parker WT, Law NW, et al. Evolving risk factors and antibiotic sensitivity patterns for microbial keratitis at a large county hospital. Br J Ophthalmol. 2017; 101(11): 1483–1487.
- Truong DT, Bui MT, Memon P, et al. Microbial Keratitis at an Urban Public Hospital: A 10-Year Update. J Clin Exp Ophthalmol. 2015; 6(6).
- Hauzer A.R., Sriram P. Severe Pseudomonas Aeruginosa Infections. Tackling the Conundrum of Drug Resistance. Postgrad Med. 2005;117(1):41-48. msvitu.com /archive/2006/april/article-1.php?print=1.
- Zimmerman AB, Nixon AD, Rueff EM. Contact lens associated microbial keratitis: practical considerations for the optometrist. Clin Optom (Auckl). 2016; 8: 1–12.
- Marquart ME. Animal models of bacterial keratitis. J Biomed Biotechnol. 2011; 2011: 680642.
- McClellan S, Jiang X, Barrett R, et al. High-mobility group box 1: a novel target for treatment of Pseudomonas aeruginosa keratitis. J Immunol. 2015; 194(4): 1776–1787.
- Kilic BB, Altiors DD, Demirbilek M, et al. Comparison between corneal cross-linking, topical antibiotic and combined therapy in experimental bacterial keratitis model. Saudi J Ophthalmol. 2018; 32(2): 97–104.
- Petrunya AM, Kutajni, M.A MA. [Study of clinical inflammatory signs in a cornea at simultaneous experimental keratitis and conjunctivitis]. Oftalmologicheskij zhurnal [J. Ophthal.]. 2013; 2: 83–88.
- Vovk IM, Kryvetska NV, Burkot VM, et al. Microbiological grounds for antimicrobial treatment of experimental pseudomonal keratitis. Rep Vinnytsia Nat Med Univ. 2020; 24(1): 114–117.
- Malachkova NV, Kryvetska NV, Vovk IM, Kryvetskiy VF. Patent Ukraine 141155. State Patent Office of Ukraine, Kyiv 2020.
- Malachkova NV, Kryvetska NV, Vovk IM, Kryvetskiy VF. Patent Ukraine 141156. State Patent Office of Ukraine, Kyiv 2020.