open access
The use of redox polymers in labelling procedures of proteins and peptides with 99mTc. I. Properties of redox polymers and technique of labelling
open access
Abstract
METHODS: Preparation for labelling takes place directly in basic protein and peptide solutions without adjusting pH or adding other adjuvants. Prior to the final modification into kit form, the redox polymers are removed by ultrafiltration.
RESULTS: The results of labelling of model compounds (aspartic acid, cysteine) with 99mTc at various solution pH values clearly show that, at pH values over 6.0, it will be primarily the free end SH groups that will serve as binding sites for the proteins and peptides. To label proteins and peptides, we selected a redox polymer with G-25 cross-linking, which allows the achievement of a radiochemical purity over 95% and high stability of the labelled compounds.
CONCLUSION: The method of radiolabelling compounds with 99mTc based on redox polymers was developed mainly to overcome the problems faced when using other conventional methods in the labelling of sensitive compounds.
Abstract
METHODS: Preparation for labelling takes place directly in basic protein and peptide solutions without adjusting pH or adding other adjuvants. Prior to the final modification into kit form, the redox polymers are removed by ultrafiltration.
RESULTS: The results of labelling of model compounds (aspartic acid, cysteine) with 99mTc at various solution pH values clearly show that, at pH values over 6.0, it will be primarily the free end SH groups that will serve as binding sites for the proteins and peptides. To label proteins and peptides, we selected a redox polymer with G-25 cross-linking, which allows the achievement of a radiochemical purity over 95% and high stability of the labelled compounds.
CONCLUSION: The method of radiolabelling compounds with 99mTc based on redox polymers was developed mainly to overcome the problems faced when using other conventional methods in the labelling of sensitive compounds.
Keywords
redox polymers; technetium (99mTc); labelling procedure; chelating groups
Title
The use of redox polymers in labelling procedures of proteins and peptides with 99mTc. I. Properties of redox polymers and technique of labelling
Journal
Issue
Pages
65-68
Published online
2000-02-25
Page views
671
Article views/downloads
1758
Bibliographic record
Nucl. Med. Rev 2000;3(1):65-68.
Keywords
redox polymers
technetium (99mTc)
labelling procedure
chelating groups
Authors
Ivan Kleisner
Pavel Komárek
Ivana Komárková
Marie Konopková