open access

Vol 3, No 1 (2000)
Basic sciences
Published online: 2000-02-25
Submitted: 2012-01-23
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The use of redox polymers in labelling procedures of proteins and peptides with 99mTc. I. Properties of redox polymers and technique of labelling

Ivan Kleisner, Pavel Komárek, Ivana Komárková, Marie Konopková
Nucl. Med. Rev 2000;3(1):65-68.

open access

Vol 3, No 1 (2000)
Basic sciences
Published online: 2000-02-25
Submitted: 2012-01-23

Abstract

BACKGROUND: Using a polymer-analogue reaction, redox polymers with a dextran matrix to anchor the end α(β)-alanine-N,N'-diacetate group converted into the Sn2+ cycle have been developed for the labelling of proteins, peptides, and labile compounds with 99mTc. The reaction kinetics and the labelling efficiency of 99mTc depend primarily on the degree of dextran matrix cross-linking and the qualitative nature of the redox polymer end groups.
METHODS: Preparation for labelling takes place directly in basic protein and peptide solutions without adjusting pH or adding other adjuvants. Prior to the final modification into kit form, the redox polymers are removed by ultrafiltration.
RESULTS: The results of labelling of model compounds (aspartic acid, cysteine) with 99mTc at various solution pH values clearly show that, at pH values over 6.0, it will be primarily the free end SH groups that will serve as binding sites for the proteins and peptides. To label proteins and peptides, we selected a redox polymer with G-25 cross-linking, which allows the achievement of a radiochemical purity over 95% and high stability of the labelled compounds.
CONCLUSION: The method of radiolabelling compounds with 99mTc based on redox polymers was developed mainly to overcome the problems faced when using other conventional methods in the labelling of sensitive compounds.

Abstract

BACKGROUND: Using a polymer-analogue reaction, redox polymers with a dextran matrix to anchor the end α(β)-alanine-N,N'-diacetate group converted into the Sn2+ cycle have been developed for the labelling of proteins, peptides, and labile compounds with 99mTc. The reaction kinetics and the labelling efficiency of 99mTc depend primarily on the degree of dextran matrix cross-linking and the qualitative nature of the redox polymer end groups.
METHODS: Preparation for labelling takes place directly in basic protein and peptide solutions without adjusting pH or adding other adjuvants. Prior to the final modification into kit form, the redox polymers are removed by ultrafiltration.
RESULTS: The results of labelling of model compounds (aspartic acid, cysteine) with 99mTc at various solution pH values clearly show that, at pH values over 6.0, it will be primarily the free end SH groups that will serve as binding sites for the proteins and peptides. To label proteins and peptides, we selected a redox polymer with G-25 cross-linking, which allows the achievement of a radiochemical purity over 95% and high stability of the labelled compounds.
CONCLUSION: The method of radiolabelling compounds with 99mTc based on redox polymers was developed mainly to overcome the problems faced when using other conventional methods in the labelling of sensitive compounds.
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Keywords

redox polymers; technetium (99mTc); labelling procedure; chelating groups

About this article
Title

The use of redox polymers in labelling procedures of proteins and peptides with 99mTc. I. Properties of redox polymers and technique of labelling

Journal

Nuclear Medicine Review

Issue

Vol 3, No 1 (2000)

Pages

65-68

Published online

2000-02-25

Bibliographic record

Nucl. Med. Rev 2000;3(1):65-68.

Keywords

redox polymers
technetium (99mTc)
labelling procedure
chelating groups

Authors

Ivan Kleisner
Pavel Komárek
Ivana Komárková
Marie Konopková

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