Vol 6, No 2 (2003)
Submitted: 2012-01-23
Published online: 2003-10-10
Diagnostic sensitivity of two radio receptor assays (TRAK Assay and TRAK Dyno Human) for detection of TSH receptor antibodies
Nebojsa Paunkovic, Jane Paunkovic
Nucl. Med. Rev 2003;6(2):119-122.
Vol 6, No 2 (2003)
Submitted: 2012-01-23
Published online: 2003-10-10
Abstract
Radio receptor assays for the detection of TSH receptor antibodies
in serum are typically based on binding the competition
of TSH-R antibodies and 125I “labeled” TSH for membrane preparation
of thyrocytes (TBII tests). The sensitivity of the available
tests utilizing porcine cell membranes was found to be around
80%. A new test (TRAK Dyno human, BRAHMS) utilizes human
recombinant TSH receptor and human standard material that
is supposed to improve the performance of the test. We have
compared the results of these two assays. The sensitivity of the
TRAK Assay tested in 356 patients with untreated Graves’ disease
was found to be 85%, and 97.5% for TRAK Dyno human in
111 newly diagnosed patients. Both tests were performed from
the same serum specimen for 60 of the investigated patients.
The TRAK Assay was positive in 50 patients (83.2%) and TRAK
Dyno human in 59 patients (98.3%). The specificity of the new
radio receptor assay was also improved.
Abstract
Radio receptor assays for the detection of TSH receptor antibodies
in serum are typically based on binding the competition
of TSH-R antibodies and 125I “labeled” TSH for membrane preparation
of thyrocytes (TBII tests). The sensitivity of the available
tests utilizing porcine cell membranes was found to be around
80%. A new test (TRAK Dyno human, BRAHMS) utilizes human
recombinant TSH receptor and human standard material that
is supposed to improve the performance of the test. We have
compared the results of these two assays. The sensitivity of the
TRAK Assay tested in 356 patients with untreated Graves’ disease
was found to be 85%, and 97.5% for TRAK Dyno human in
111 newly diagnosed patients. Both tests were performed from
the same serum specimen for 60 of the investigated patients.
The TRAK Assay was positive in 50 patients (83.2%) and TRAK
Dyno human in 59 patients (98.3%). The specificity of the new
radio receptor assay was also improved.
Keywords
radioreceptor assay; human receptor; TSH receptor antibody; Graves’ disease
Title
Diagnostic sensitivity of two radio receptor assays (TRAK Assay and TRAK Dyno Human) for detection of TSH receptor antibodies
Journal
Nuclear Medicine Review
Issue
Vol 6, No 2 (2003)
Pages
119-122
Published online
2003-10-10
Page views
576
Article views/downloads
1247
Bibliographic record
Nucl. Med. Rev 2003;6(2):119-122.
Keywords
radioreceptor assay
human receptor
TSH receptor antibody
Graves’ disease
Authors
Nebojsa Paunkovic
Jane Paunkovic