open access

Vol 6, No 2 (2003)
Published online: 2003-10-10
Submitted: 2012-01-23
Get Citation

Diagnostic sensitivity of two radio receptor assays (TRAK Assay and TRAK Dyno Human) for detection of TSH receptor antibodies

Nebojsa Paunkovic, Jane Paunkovic
Nucl. Med. Rev 2003;6(2):119-122.

open access

Vol 6, No 2 (2003)
Published online: 2003-10-10
Submitted: 2012-01-23

Abstract

Radio receptor assays for the detection of TSH receptor antibodies in serum are typically based on binding the competition of TSH-R antibodies and 125I “labeled” TSH for membrane preparation of thyrocytes (TBII tests). The sensitivity of the available tests utilizing porcine cell membranes was found to be around 80%. A new test (TRAK Dyno human, BRAHMS) utilizes human recombinant TSH receptor and human standard material that is supposed to improve the performance of the test. We have compared the results of these two assays. The sensitivity of the TRAK Assay tested in 356 patients with untreated Graves’ disease was found to be 85%, and 97.5% for TRAK Dyno human in 111 newly diagnosed patients. Both tests were performed from the same serum specimen for 60 of the investigated patients. The TRAK Assay was positive in 50 patients (83.2%) and TRAK Dyno human in 59 patients (98.3%). The specificity of the new radio receptor assay was also improved.

Abstract

Radio receptor assays for the detection of TSH receptor antibodies in serum are typically based on binding the competition of TSH-R antibodies and 125I “labeled” TSH for membrane preparation of thyrocytes (TBII tests). The sensitivity of the available tests utilizing porcine cell membranes was found to be around 80%. A new test (TRAK Dyno human, BRAHMS) utilizes human recombinant TSH receptor and human standard material that is supposed to improve the performance of the test. We have compared the results of these two assays. The sensitivity of the TRAK Assay tested in 356 patients with untreated Graves’ disease was found to be 85%, and 97.5% for TRAK Dyno human in 111 newly diagnosed patients. Both tests were performed from the same serum specimen for 60 of the investigated patients. The TRAK Assay was positive in 50 patients (83.2%) and TRAK Dyno human in 59 patients (98.3%). The specificity of the new radio receptor assay was also improved.
Get Citation

Keywords

radioreceptor assay; human receptor; TSH receptor antibody; Graves’ disease

About this article
Title

Diagnostic sensitivity of two radio receptor assays (TRAK Assay and TRAK Dyno Human) for detection of TSH receptor antibodies

Journal

Nuclear Medicine Review

Issue

Vol 6, No 2 (2003)

Pages

119-122

Published online

2003-10-10

Bibliographic record

Nucl. Med. Rev 2003;6(2):119-122.

Keywords

radioreceptor assay
human receptor
TSH receptor antibody
Graves’ disease

Authors

Nebojsa Paunkovic
Jane Paunkovic

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By "Via Medica sp. z o.o." sp.k., Świętokrzyska 73 street, 80–180 Gdańsk, Poland

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail: viamedica@viamedica.pl