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Reassessment of the reproducibility of Technetium-99m mercaptoacetyltriglycine clearance
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Abstract
MATERIAL AND METHODS: Fifty subjects underwent three successive tests performed at 1-week interval. The physiological conditions were controlled as much as possible and all experimental measurements were rigorously double-checked. Renal clearances were calculated using single sample algorithms.
RESULTS: Thin layer chromatography demonstrated a radiochemical purity of more than 92% (mean 93.8%; SD 1.6%; range 92.0–96.4%). The mean (and SD) of 44th minute plasma concentrations for the three successive measurements were respectively 1.8 ± 0.4, 2.1 ± 0.4 and 1.9 ± 0.4 (%dose/liter). The mean changes (and S.D. of differences) between two tests were 0.26 ± 0.29, –0.08 ± 0.5 and 0.18 ± 0.30 respectively between tests 1 and 2, tests 1 and 3, and tests 2 and 3. Using Bubeck’s algorithm, the mean clearance values (ml/min/1.73 m2) were 268.8 (range 201.4–336.8), 247.2 (range 170.5–290.3), and 262.8 (range 187.4–340.0) respectively for the first, second and third measurements. Using Russell’s approach, the mean clearance values were respectively 314.9 (range: 208.7–441.7), 280.1 (range: 167.5–358.6), and 305.4 (range: 189.5–447.9).
CONCLUSIONS: High differences were observed between the 3 tests. Using Russell´s formula, the SD of differences between two tests was respectively 35.8, 47.7 and 57.7 ml/min/1.73 m2 between tests 1 and 2, tests 1 and 3, and tests 2 and 3. Whether such a large variability is acceptable in clinical practice depends solely on what the clinician is expecting.
Abstract
MATERIAL AND METHODS: Fifty subjects underwent three successive tests performed at 1-week interval. The physiological conditions were controlled as much as possible and all experimental measurements were rigorously double-checked. Renal clearances were calculated using single sample algorithms.
RESULTS: Thin layer chromatography demonstrated a radiochemical purity of more than 92% (mean 93.8%; SD 1.6%; range 92.0–96.4%). The mean (and SD) of 44th minute plasma concentrations for the three successive measurements were respectively 1.8 ± 0.4, 2.1 ± 0.4 and 1.9 ± 0.4 (%dose/liter). The mean changes (and S.D. of differences) between two tests were 0.26 ± 0.29, –0.08 ± 0.5 and 0.18 ± 0.30 respectively between tests 1 and 2, tests 1 and 3, and tests 2 and 3. Using Bubeck’s algorithm, the mean clearance values (ml/min/1.73 m2) were 268.8 (range 201.4–336.8), 247.2 (range 170.5–290.3), and 262.8 (range 187.4–340.0) respectively for the first, second and third measurements. Using Russell’s approach, the mean clearance values were respectively 314.9 (range: 208.7–441.7), 280.1 (range: 167.5–358.6), and 305.4 (range: 189.5–447.9).
CONCLUSIONS: High differences were observed between the 3 tests. Using Russell´s formula, the SD of differences between two tests was respectively 35.8, 47.7 and 57.7 ml/min/1.73 m2 between tests 1 and 2, tests 1 and 3, and tests 2 and 3. Whether such a large variability is acceptable in clinical practice depends solely on what the clinician is expecting.
Keywords
Tc-99m MAG3; clearance; precision; reproducibility; Russell; Bubeck
Title
Reassessment of the reproducibility of Technetium-99m mercaptoacetyltriglycine clearance
Journal
Issue
Article type
Research paper
Pages
15-20
Published online
2005-06-21
Page views
476
Article views/downloads
1143
Bibliographic record
Nucl. Med. Rev 2005;8(1):15-20.
Keywords
Tc-99m MAG3
clearance
precision
reproducibility
Russell
Bubeck
Authors
Carlos De Sadeleer