open access

Vol 10, No 2 (2007)
Basic sciences
Published online: 2007-05-23
Submitted: 2012-01-23
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Evaluation of [67Ga]-insulin for insulin receptor imaging

Amir Reza Jalilian, Javad Garosi, Ebrahim Gholami, Mehdi Akhlaghi, Fariba Saddadi, Fatemeh Bolourinovin, Alireza Karimian
Nucl. Med. Rev 2007;10(2):71-75.

open access

Vol 10, No 2 (2007)
Basic sciences
Published online: 2007-05-23
Submitted: 2012-01-23

Abstract

BACKGROUND: Radiolabelled human recombinant insulin can be used for the imaging of insulin receptors in some tumours where FDG has natural uptake and diminishes the value of its imaging.
MATERIAL AND METHODS: Insulin was successively labelled with [67 Ga]-gallium chloride after conjugation with freshly prepared cyclic DTPA-dianhydride (HPLC radiochemical purity assay > 96%) followed by biodistribution studies in normal rats, white blood cell labelling and preliminary SPECT studies.
RESULTS: In vitro studies demonstrated the retention of radiolabelled insulin receptor affinity using freshly prepared human white blood cells at different blood sugar conditions. Preliminary in vivo studies in a normal rat model was performed to determine the biodistribution of the radioimmunoconjugate at up to 44 h. SPECT images revealed high uptake of the liver.
CONCLUSION: Radiolabelled insulin is stable enough to be used in biological studies in order to image insulin receptors in diabetic conditions as well as possible tumour imaging applications. The data was consistent with other radiolabelled insulin studies.

Abstract

BACKGROUND: Radiolabelled human recombinant insulin can be used for the imaging of insulin receptors in some tumours where FDG has natural uptake and diminishes the value of its imaging.
MATERIAL AND METHODS: Insulin was successively labelled with [67 Ga]-gallium chloride after conjugation with freshly prepared cyclic DTPA-dianhydride (HPLC radiochemical purity assay > 96%) followed by biodistribution studies in normal rats, white blood cell labelling and preliminary SPECT studies.
RESULTS: In vitro studies demonstrated the retention of radiolabelled insulin receptor affinity using freshly prepared human white blood cells at different blood sugar conditions. Preliminary in vivo studies in a normal rat model was performed to determine the biodistribution of the radioimmunoconjugate at up to 44 h. SPECT images revealed high uptake of the liver.
CONCLUSION: Radiolabelled insulin is stable enough to be used in biological studies in order to image insulin receptors in diabetic conditions as well as possible tumour imaging applications. The data was consistent with other radiolabelled insulin studies.
Get Citation

Keywords

insulin; gallium-67; radiolabelling; biodistribution; WBC binding; SPECT

About this article
Title

Evaluation of [67Ga]-insulin for insulin receptor imaging

Journal

Nuclear Medicine Review

Issue

Vol 10, No 2 (2007)

Pages

71-75

Published online

2007-05-23

Bibliographic record

Nucl. Med. Rev 2007;10(2):71-75.

Keywords

insulin
gallium-67
radiolabelling
biodistribution
WBC binding
SPECT

Authors

Amir Reza Jalilian
Javad Garosi
Ebrahim Gholami
Mehdi Akhlaghi
Fariba Saddadi
Fatemeh Bolourinovin
Alireza Karimian

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