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Evaluation of [67Ga]-insulin for insulin receptor imaging
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Abstract
MATERIAL AND METHODS: Insulin was successively labelled with [67 Ga]-gallium chloride after conjugation with freshly prepared cyclic DTPA-dianhydride (HPLC radiochemical purity assay > 96%) followed by biodistribution studies in normal rats, white blood cell labelling and preliminary SPECT studies.
RESULTS: In vitro studies demonstrated the retention of radiolabelled insulin receptor affinity using freshly prepared human white blood cells at different blood sugar conditions. Preliminary in vivo studies in a normal rat model was performed to determine the biodistribution of the radioimmunoconjugate at up to 44 h. SPECT images revealed high uptake of the liver.
CONCLUSION: Radiolabelled insulin is stable enough to be used in biological studies in order to image insulin receptors in diabetic conditions as well as possible tumour imaging applications. The data was consistent with other radiolabelled insulin studies.
Abstract
MATERIAL AND METHODS: Insulin was successively labelled with [67 Ga]-gallium chloride after conjugation with freshly prepared cyclic DTPA-dianhydride (HPLC radiochemical purity assay > 96%) followed by biodistribution studies in normal rats, white blood cell labelling and preliminary SPECT studies.
RESULTS: In vitro studies demonstrated the retention of radiolabelled insulin receptor affinity using freshly prepared human white blood cells at different blood sugar conditions. Preliminary in vivo studies in a normal rat model was performed to determine the biodistribution of the radioimmunoconjugate at up to 44 h. SPECT images revealed high uptake of the liver.
CONCLUSION: Radiolabelled insulin is stable enough to be used in biological studies in order to image insulin receptors in diabetic conditions as well as possible tumour imaging applications. The data was consistent with other radiolabelled insulin studies.
Keywords
insulin; gallium-67; radiolabelling; biodistribution; WBC binding; SPECT
About this articleTitle
Evaluation of [67Ga]-insulin for insulin receptor imaging
Journal
Issue
Pages
71-75
Published online
2007-05-23
Page views
902
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1649
Bibliographic record
Nucl. Med. Rev 2007;10(2):71-75.
Keywords
insulin
gallium-67
radiolabelling
biodistribution
WBC binding
SPECT
Authors
Amir Reza Jalilian
Javad Garosi
Ebrahim Gholami
Mehdi Akhlaghi
Fariba Saddadi
Fatemeh Bolourinovin
Alireza Karimian
