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Published online: 2022-06-10
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Cyclooxygenase-2 and Bcl-2 expression in patients with triple-negative breast cancer

Anna Liszcz-Tymoszuk12, Marta Fudalej1, Andrzej Deptała1, Anna Maria Badowska-Kozakiewicz1
DOI: 10.5603/NJO.a2022.0035
Affiliations
  1. Department of Cancer Prevention, Medical University of Warsaw, Warsaw, Poland
  2. Students’ Scientific Organization of Cancer Cell Biology, Department of Cancer Prevention, Medical University of Warsaw, Warsaw, Poland

open access

Ahead of print
Original article
Published online: 2022-06-10

Abstract

Introduction. Triple-negative breast cancer (TNBC) is a rare type of breast cancer associated with lack of expression of estrogen and progesterone receptors and HER2 protein, characterized by poor outcome and chemotherapy resistance. Cyclooxygenase-2 (COX-2) is a constitutional enzyme responsible for prostaglandin synthesis, present in neoplastic cells and premalignant lesions. B-cell lymphoma 2 (Bcl-2) protein is considered to be one of the most potent apoptosis-regulating agents, assuring body homeostasis.

Aim. The aim of the present study was to evaluate the immunohistochemical (IHC) profile of COX-2 and Bcl-2 expression in patients suffering from TNBC in order to obtain more detailed data on additional factors negatively influencing TNBC outcome.

Material and methods. The IHC evaluation of COX-2 and Bcl-2 expression among 21 women with diagnosis of TNBC was performed.

Results. The most common histological subtype was invasive ductal cancer of no special type. COX-2 was present in all examined samples with moderate to strong expression detected in 20 of 21 cases. There was a positive correlation between histological grade (G) and COX-2 expression (p=0.002). Bcl-2 was present in all examined samples. The analysis has shown that tumours presenting highy positive expression of Bcl-2 accounted for the majority of examined cases (57.2%).

Conclusions. Achieved results might lead to a conclusion that COX-2 and Bcl-2 high expression in TNBC may be linked with tumour aggressiveness and poor overall survival. However, before their consideration as additional markers used in routine histological examination and grading in breast cancer, further studies are necessary.

Abstract

Introduction. Triple-negative breast cancer (TNBC) is a rare type of breast cancer associated with lack of expression of estrogen and progesterone receptors and HER2 protein, characterized by poor outcome and chemotherapy resistance. Cyclooxygenase-2 (COX-2) is a constitutional enzyme responsible for prostaglandin synthesis, present in neoplastic cells and premalignant lesions. B-cell lymphoma 2 (Bcl-2) protein is considered to be one of the most potent apoptosis-regulating agents, assuring body homeostasis.

Aim. The aim of the present study was to evaluate the immunohistochemical (IHC) profile of COX-2 and Bcl-2 expression in patients suffering from TNBC in order to obtain more detailed data on additional factors negatively influencing TNBC outcome.

Material and methods. The IHC evaluation of COX-2 and Bcl-2 expression among 21 women with diagnosis of TNBC was performed.

Results. The most common histological subtype was invasive ductal cancer of no special type. COX-2 was present in all examined samples with moderate to strong expression detected in 20 of 21 cases. There was a positive correlation between histological grade (G) and COX-2 expression (p=0.002). Bcl-2 was present in all examined samples. The analysis has shown that tumours presenting highy positive expression of Bcl-2 accounted for the majority of examined cases (57.2%).

Conclusions. Achieved results might lead to a conclusion that COX-2 and Bcl-2 high expression in TNBC may be linked with tumour aggressiveness and poor overall survival. However, before their consideration as additional markers used in routine histological examination and grading in breast cancer, further studies are necessary.

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Keywords

Bcl-2; cyclooxygenase-2; immunohistochemistry; prognostic factors; triple-negative breast cancer

About this article
Title

Cyclooxygenase-2 and Bcl-2 expression in patients with triple-negative breast cancer

Journal

Nowotwory. Journal of Oncology

Issue

Ahead of print

Article type

Research paper (original)

Published online

2022-06-10

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24

Article views/downloads

11

DOI

10.5603/NJO.a2022.0035

Keywords

Bcl-2
cyclooxygenase-2
immunohistochemistry
prognostic factors
triple-negative breast cancer

Authors

Anna Liszcz-Tymoszuk
Marta Fudalej
Andrzej Deptała
Anna Maria Badowska-Kozakiewicz

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