Lipase B from Candida antarctica — the wide applicable biocatalyst in obtaining pharmaceutical compounds
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Abstract
Lipases are commonly applied in the pharmaceutical and chemical industry, especially in immobilized
form. The use of immobilized lipases facilitates the design of reactors and control of reactions, for example,
fast stopping the reaction. The immobilization procedure should increase the stability of the lipase
and its activity, as well as be simple and efficient. Lipase B from Candida antarctica (CAL-B) is an enzyme
from the lipase group, isolated from the Candida antarctica species. CAL-B has the highest activity in
non-polar organic solvents, such as hexane and toluene, and the lowest in polar solvents, e.g. acetonitrile.
Due to its hydrolytic properties, this enzyme degrades triglycerides of fatty acids to free fatty acids (FFA)
and glycerol. Described lipase is often immobilized, in the aim to increase enantioselective and lipolytic
activity. The kinetic and dynamic resolution with the application of lipase is one of the ways in obtaining
an enantiopure form of the drugs, which usually are more effective and safer for the patient. The CAL-B
could be also applied in the kinetic resolution of compounds being building blocks, derivates of drugs or
conjugated forms. Furthermore, the CAL-B is used in the reactions in receiving of organic compounds,
which are the natural origin, especially vegetable. Based on the presented data, it can be concluded, that
CAL-B is an enzyme with a wide application in the biosynthesis of compounds with therapeutic activity.
Keywords: lipase B from Candida antarcticaimmobilizationtriglyceridesinterfacial activationkinetic resolutiontransesterificationacylation
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