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Vol 4, No 2 (2019)
ORIGINAL ARTICLES
Published online: 2019-04-09
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The effect of initial sonication on disinfectant efficacy against Listeria monocytogenes biofilm

Natalia Wiktorczyk, Jakub Korkus, Krzysztof Skowron, Ewa Wałecka-Zacharska, Eugenia Gospodarek-Komkowska
DOI: 10.5603/MRJ.a2019.0020
·
Medical Research Journal 2019;4(2):85-88.

open access

Vol 4, No 2 (2019)
ORIGINAL ARTICLES
Published online: 2019-04-09

Abstract

Background: Listeria monocytogenes is a Gram-positive, foodborne pathogen. Biofilms formed by this
bacterium are a serious problem in the food industry. Bacteria in biofilms are much more resistant to cleaning
and disinfection agents posing a risk of food recontamination. The aim of this study was the assessment
of the influence of initial sonication on disinfectant efficacy, based on QAC, against L. monocytogenes
biofilm on the stainless steel.
Methods: The biofilm formed on the stainless steel by the reference strain L. monocytogenes ATCC 19111
was sonicated for 1 and 5 minutes (500W/ 20kHz/ 100% amplitude). Then disinfection with quaternary
ammonium compounds (0.5% working solution) was applied for 1 and 5 minutes and the number of
bacteria recovered from the biofilm was assessed.
Results: It was found that disinfection was more efficient than sonication (p ≤ 0.05). However, the combination
of sonication and disinfection significantly improved biofilm eradication compared to the use of
one of these methods separately (p ≤ 0.05). The greatest reduction of bacteria number was achieved after
5 minutes of sonication combined with 5 minutes of disinfection (6.42 log CFU × cm-2), whereas the lowest
reduction was observed after 1 minute-sonication (2.03 log CFU × cm-2).
Conclusions: Combination of sonication and disinfection based on quaternary ammonium compounds is
an effective method allowing biofilm eradication from the food production surfaces.

Abstract

Background: Listeria monocytogenes is a Gram-positive, foodborne pathogen. Biofilms formed by this
bacterium are a serious problem in the food industry. Bacteria in biofilms are much more resistant to cleaning
and disinfection agents posing a risk of food recontamination. The aim of this study was the assessment
of the influence of initial sonication on disinfectant efficacy, based on QAC, against L. monocytogenes
biofilm on the stainless steel.
Methods: The biofilm formed on the stainless steel by the reference strain L. monocytogenes ATCC 19111
was sonicated for 1 and 5 minutes (500W/ 20kHz/ 100% amplitude). Then disinfection with quaternary
ammonium compounds (0.5% working solution) was applied for 1 and 5 minutes and the number of
bacteria recovered from the biofilm was assessed.
Results: It was found that disinfection was more efficient than sonication (p ≤ 0.05). However, the combination
of sonication and disinfection significantly improved biofilm eradication compared to the use of
one of these methods separately (p ≤ 0.05). The greatest reduction of bacteria number was achieved after
5 minutes of sonication combined with 5 minutes of disinfection (6.42 log CFU × cm-2), whereas the lowest
reduction was observed after 1 minute-sonication (2.03 log CFU × cm-2).
Conclusions: Combination of sonication and disinfection based on quaternary ammonium compounds is
an effective method allowing biofilm eradication from the food production surfaces.

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Keywords

Listeria monocytogenes, biofilm, sonication, disinfectants, quaternary ammonium compounds

About this article
Title

The effect of initial sonication on disinfectant efficacy against Listeria monocytogenes biofilm

Journal

Medical Research Journal

Issue

Vol 4, No 2 (2019)

Pages

85-88

Published online

2019-04-09

DOI

10.5603/MRJ.a2019.0020

Bibliographic record

Medical Research Journal 2019;4(2):85-88.

Keywords

Listeria monocytogenes
biofilm
sonication
disinfectants
quaternary ammonium compounds

Authors

Natalia Wiktorczyk
Jakub Korkus
Krzysztof Skowron
Ewa Wałecka-Zacharska
Eugenia Gospodarek-Komkowska

References (18)
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