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Vol 3, No 4 (2018)
Original article
Published online: 2018-12-19
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A preliminary study on MTDH expression as a potential prognostic cancer marker

Katarzyna Kaminska1, Sylwia Szablewska2, Krzysztof Roszkowski2, Marzena Anna Lewandowska3
·
Medical Research Journal 2018;3(4):211-214.
Affiliations
  1. Molecular Oncology and Genetics Departament, Innovative Medical Forum, The F. Lukaszczyk Oncology Center Bydgoszcz, Romanowskiej 2, 85-796 Bydgoszcz, Poland
  2. Department of Oncology, Radiotherapy and Gynecologic Oncology, Collegium Medicum, Nicolaus Copernicus University,, Romanowskiej 2, 85-796 Bydgoszcz
  3. Department of Thoracic Surgery and Tumors, Faculty of Medicine, Collegium Medicum, Nicolaus Copernicus University, Romanowskiej 2, 85-796 Bydgoszcz, Poland

open access

Vol 3, No 4 (2018)
ORIGINAL ARTICLES
Published online: 2018-12-19

Abstract

Background: Clinical studies have revealed that MTDH is overexpressed in various malignancies and
is associated with disease progression and poor clinical outcomes. In order to study MTDH prognostic
potential, we decided to evaluate MTDH expression changes using cancerous and non-cancerous cells
lines. Secondly, for the first time, we evaluated MTDH expression in prostate cancer cell lines representing
different metastatic potential in vivo.


Methods: MTDH and PBGD (control) genes expression were measured by reverse transcription-quantitative
polymerase chain reaction assay using Universal Probe Library in cancerous and non-cancerous cell lines.

Results: MTDH gene expression analysis showed a decrease in colorectal cancer cell lines (Caco2, HT29)
compared to non-cancerous cells lines (VH10, VH25, Hek293). The mean level of the MTDH mRNA expression,
normalized in relation to the reference gene PBGD, increased in the prostate cancer cell lines
as follows: PC3 (0.62 ± 0.07), PC3M (1.02 ± 0.17), PC3MPro4 (1.20 ± 0.22), and reached the highest
value in PC3M4 (1.86 ± 0.48). In VH10, the expression of this gene was at 1.0 ± 0.07.

Conclusions: Our MTDH gene expression data are consistent with Mtdh protein expression analyzed in
The Human Protein Atlas (HPA). Increasing MTDH expression is a promising prognostic factor.

Abstract

Background: Clinical studies have revealed that MTDH is overexpressed in various malignancies and
is associated with disease progression and poor clinical outcomes. In order to study MTDH prognostic
potential, we decided to evaluate MTDH expression changes using cancerous and non-cancerous cells
lines. Secondly, for the first time, we evaluated MTDH expression in prostate cancer cell lines representing
different metastatic potential in vivo.


Methods: MTDH and PBGD (control) genes expression were measured by reverse transcription-quantitative
polymerase chain reaction assay using Universal Probe Library in cancerous and non-cancerous cell lines.

Results: MTDH gene expression analysis showed a decrease in colorectal cancer cell lines (Caco2, HT29)
compared to non-cancerous cells lines (VH10, VH25, Hek293). The mean level of the MTDH mRNA expression,
normalized in relation to the reference gene PBGD, increased in the prostate cancer cell lines
as follows: PC3 (0.62 ± 0.07), PC3M (1.02 ± 0.17), PC3MPro4 (1.20 ± 0.22), and reached the highest
value in PC3M4 (1.86 ± 0.48). In VH10, the expression of this gene was at 1.0 ± 0.07.

Conclusions: Our MTDH gene expression data are consistent with Mtdh protein expression analyzed in
The Human Protein Atlas (HPA). Increasing MTDH expression is a promising prognostic factor.

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Keywords

MTDH (Metadherin), prostate cancer cell lines, gene expression

About this article
Title

A preliminary study on MTDH expression as a potential prognostic cancer marker

Journal

Medical Research Journal

Issue

Vol 3, No 4 (2018)

Article type

Original article

Pages

211-214

Published online

2018-12-19

Page views

933

Article views/downloads

764

DOI

10.5603/MRJ.a2018.0036

Bibliographic record

Medical Research Journal 2018;3(4):211-214.

Keywords

MTDH (Metadherin)
prostate cancer cell lines
gene expression

Authors

Katarzyna Kaminska
Sylwia Szablewska
Krzysztof Roszkowski
Marzena Anna Lewandowska

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