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Can bone marrow mesenchymal stem cells regenerate the myocardium?
Affiliations- Department of Tissue Engineering, Collegium Medicum Bydgoszcz, Nicolaus Copernicus University, Karlowicza 24, 85-092 Bydgoszcz, Poland
- Department of Pediatric Hematology and Oncology, Collegium Medicum Bydgoszcz, Nicolaus Copernicus University, Curie Sklodowskiej 9, 85-094 Bydgoszcz, Poland
- Department of Histology and Embryology, Collegium Medicum Bydgoszcz, Nicolaus Copernicus University, Curie-Sklodowskiej 9, 85-094 Bydgoszcz, Poland
- Department of Clinical Pathomorphology, Collegium Medicum Bydgoszcz, Nicolaus Copernicus University,, Curie-Sklodowskiej 9, 85-094 Bydgoszcz, Poland
- Department of Oncology, Radiotherapy and Ginecologic Oncology, Collegium Medicum Bydgoszcz, Nicolaus Copernicus University, Romanowskiej 2, 85-796 Bydgoszcz, Poland
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Abstract
Background: Cardiovascular diseases are the serious clinical problem, especially the loss of viable myocytes.
A new approach, which provides a novel method for the treatment, is a tissue engineering and
regenerative medicine. One of the current cell types used as a source to improve cardiac tissue repair,
are MSCs. The aim of this study was to check if the 5’azacitidine and growth factors from cardiomyocyte
cell line initiate the differentiation of MSCs toward cardiomyocytes.
Material and methods: Bone marrow MSCs were isolated and their biological features have been characterized.
Conditioned media were prepared with the use of 5’azacitidine and growth factors released
from cardiomyocytes.
Results: The transdifferentiating process has been confirmed by the expression of specific muscle markers.
Conditioned medium from cardiomyocyte cell line, as well as, 5’azacitidine induced muscle differentiation
process in bone marrow MSCs in comparison to the control.
Conclusions: Presented in this study data support the conclusion that this concept may represent a promising
strategy for the repair of cardiac tissue, however, further experiments are necessary.
Abstract
Background: Cardiovascular diseases are the serious clinical problem, especially the loss of viable myocytes.
A new approach, which provides a novel method for the treatment, is a tissue engineering and
regenerative medicine. One of the current cell types used as a source to improve cardiac tissue repair,
are MSCs. The aim of this study was to check if the 5’azacitidine and growth factors from cardiomyocyte
cell line initiate the differentiation of MSCs toward cardiomyocytes.
Material and methods: Bone marrow MSCs were isolated and their biological features have been characterized.
Conditioned media were prepared with the use of 5’azacitidine and growth factors released
from cardiomyocytes.
Results: The transdifferentiating process has been confirmed by the expression of specific muscle markers.
Conditioned medium from cardiomyocyte cell line, as well as, 5’azacitidine induced muscle differentiation
process in bone marrow MSCs in comparison to the control.
Conclusions: Presented in this study data support the conclusion that this concept may represent a promising
strategy for the repair of cardiac tissue, however, further experiments are necessary.
Keywords
cell therapy, cardiovascular disease, mesenchymal stem cells, tissue engineering
About this articleTitle
Can bone marrow mesenchymal stem cells regenerate the myocardium?
Journal
Issue
Article type
Original article
Pages
153-158
Published online
2018-11-02
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937
Article views/downloads
724
DOI
Bibliographic record
Medical Research Journal 2018;3(3):153-158.
Keywords
cell therapy
cardiovascular disease
mesenchymal stem cells
tissue engineering
Authors
Łukasz Kaźmierski
Anna Bajek
Robert Dębski
Maciej Gagat
Alina Grzanka
Magdalena Bodnar
Krzysztof Roszkowski
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