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Icaritin induces apoptosis and downregulates RhoA/ROCK pathway in the A549 adenocarcinoma cell line
, 1
Affiliations- Department of Histology and Embryology, Faculty of Medicine, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Poland
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Abstract
Introduction: Icaritin is a prenylflavonoid derivative from a traditional Chinese herb, Epimedium Genus. Icaritin exerts its anti-tumour effects in various cancers mainly by inhibiting cell proliferation, inducing cell differentiation and apoptosis, and suppressing cell migration and invasion. The study aimed to evaluate the effect of icaritin on apoptosis, cell cycle, and expression of selected cell cycle proteins: RhoA, ROCK1, and ROCK2 in the A549 cell line.
Methods: Apoptosis and the cell cycle distribution were evaluated by flow cytometry. Protein expression was measured by conducting a western blot assay and immunofluorescence staining.
Results and conclusions: The study revealed that icaritin induces apoptosis and cell cycle arrest in the G2/M phase in A549 cells. The authors showed that icaritin activates the p53 protein and downregulates Rho/ROCK pathway. Additionally, the decrease in expression of thrombospondin-1 was observed which can be part of the cytoprotective response of A549 cells on ICT treatment.
Abstract
Introduction: Icaritin is a prenylflavonoid derivative from a traditional Chinese herb, Epimedium Genus. Icaritin exerts its anti-tumour effects in various cancers mainly by inhibiting cell proliferation, inducing cell differentiation and apoptosis, and suppressing cell migration and invasion. The study aimed to evaluate the effect of icaritin on apoptosis, cell cycle, and expression of selected cell cycle proteins: RhoA, ROCK1, and ROCK2 in the A549 cell line.
Methods: Apoptosis and the cell cycle distribution were evaluated by flow cytometry. Protein expression was measured by conducting a western blot assay and immunofluorescence staining.
Results and conclusions: The study revealed that icaritin induces apoptosis and cell cycle arrest in the G2/M phase in A549 cells. The authors showed that icaritin activates the p53 protein and downregulates Rho/ROCK pathway. Additionally, the decrease in expression of thrombospondin-1 was observed which can be part of the cytoprotective response of A549 cells on ICT treatment.
Keywords
RhoA, ROCK1, ROCK2, lung cancer, icaritin (ICT), cell death, thrombospondin-1
About this articleTitle
Icaritin induces apoptosis and downregulates RhoA/ROCK pathway in the A549 adenocarcinoma cell line
Journal
Issue
Article type
Original article
Pages
242-248
Published online
2022-09-30
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3769
Article views/downloads
293
DOI
Bibliographic record
Medical Research Journal 2022;7(3):242-248.
Keywords
RhoA
ROCK1
ROCK2
lung cancer
icaritin (ICT)
cell death
thrombospondin-1
Authors
Agnieszka Żuryń
Adrian Krajewski
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