open access
Kinetic resolution of a b-adrenolytic drug with the use of lipases as enantioselective biocatalysts
open access
Abstract
The study presented herein is focused on optimisation of the kinetic resolution of racemic propranolol by screening acetylating agent, reaction medium, and enantioselective biocatalysts. The effects of acetylating agent and reaction medium on the efficiency of performed enantioselective biotransformation were inves-tigated. Furthermore, the catalytic activities on nine commercially available lipases were tested. Finally, the composition of reaction medium was selected, based on previously obtained results, providing the most efficient process of kinetic resolution of racemic propranolol.
Abstract
The study presented herein is focused on optimisation of the kinetic resolution of racemic propranolol by screening acetylating agent, reaction medium, and enantioselective biocatalysts. The effects of acetylating agent and reaction medium on the efficiency of performed enantioselective biotransformation were inves-tigated. Furthermore, the catalytic activities on nine commercially available lipases were tested. Finally, the composition of reaction medium was selected, based on previously obtained results, providing the most efficient process of kinetic resolution of racemic propranolol.
Keywords
enzyme, (R,S)-propranolol, kinetic resolution, enantioselective acetylation, Candida rugosa (OF) lipase
About this articleTitle
Kinetic resolution of a b-adrenolytic drug with the use of lipases as enantioselective biocatalysts
Journal
Issue
Article type
Technical note
Pages
38-42
Published online
2018-03-20
Page views
943
Article views/downloads
1173
DOI
Bibliographic record
Medical Research Journal 2018;3(1):38-42.
Keywords
enzyme
(R
S)-propranolol
kinetic resolution
enantioselective acetylation
Candida rugosa (OF) lipase
Authors
Adam Sikora
Agata Tarczykowska
Joanna Chałupka
Michał Piotr Marszałł
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