Impact of fractalkine polymorphisms (rs 614230, rs 170364) on the occurrence of inappropriate body mass
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Abstract
Introduction: Fractalkine chemokine (CX3CL1) is a protein cytokine which is associated with the pathophysiology of lifestyle-related diseases such as obesity, diabetes and atherosclerosis. This study aimed to assess the influence of fractalkine gene variants (rs 614230, rs 170364) on body mass.
Material and methods: Study group consisted of 295 patients (152 women and 133 men). The blood samples were collected and stored at –70˚C. Afterwards the DNA material was isolated and prepared (quality and quantity were checked by spectrophotometry). Allelic discrimination of fractalkine gene (rs 614230, rs 170364) was performed using Roche Lightcycler96 thermocycler. Real-Time PCR was conducted using fluorescence-labeled TaqMan Pre-designed SNP Genotyping Assay probes.
Results: The CC genotype of fractalkine (rs 614230) had a positive association with BMI compared to CT and TT genotypes. The GG genotype of fractalkine (rs 170364) had a negative association with body size, body mass index and body fat content compared to GT. The TT genotype of fractalkine (rs 170364) shows a negative association with BMI compared to GT.
Conclusions: Fractalkine polymorphism rs 614230 may be associated with high body mass index (BMI), but not with waist size or body fat content. Single nucleotide polymorphisms (SNP) of fractalkine rs 170364 may be associated with high body mass. Further studies in a larger group of patients are necessary to completely assess this association.
Keywords: fractalkineobesitysingle nucleotide polymorphisms
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