Biochemical markers of oxidative stress in patients with inflammatory bowel diseases
Abstract
Introduction: Inflammatory bowel disease (IBD) is a group of diseases of unexplained aetiology, characterized by periods of remissions and exacerbations. Reactive oxygen species (ROS) as far as disorders of balance between levels of prooxidants and antioxidants may also participate in the occurrence of IBD. The aim of the present study was an assessment of the antioxidative barrier of the organism in patients with inflammatory bowel disease.
Material and methods: The study group consisted of 99 patients (80 with IBD as a study group and 19 healthy as a control group) from Jan Biziel University Hospital in Bydgoszcz, Poland. Venous blood was the material for biochemical analysis: HT, GSH, GPXp, GPXRBC, GST, GR, SOD-1, MDA, NO2-/NO3- and CP.
Results: There were statistically significant differences in oxidative stress parameters observed between the study group and the control group, especially concerning HT, GSH, GPXRBC, GST, SOD-1, MDA and NO2-/NO3-.
Discussion: The assumption that increased activity of antioxidative compounds may constitute a defence against the influence of oxidative stress may be true. Their decreased activity may participate in lowering an organism’s abilities to defend against oxidative stress and cause the development of free radical diseases. Further studies into targeted preventive strategies are needed.
Conclusions: Prooxidative factors play an essential role in the pathogenesis of IBD. Due to the still unknown etiopathology of IBD, research on imbalances between pro-oxidants and antioxidants should be continued in larger groups of patients.
Keywords: oxidative stressantioxidative barrierinflammatory bowel disease
References
- Weiss G, Gasche C. Pathogenesis and treatment of anemia in inflammatory bowel disease. Haematologica. 2010; 95(2): 175–178.
- Zhang Y, Ikeno Y, Qi W, et al. The role of oxidative damage and stress in aging. Mech Ageing Dev. 2004; 125(10-11): 811–826.
- Vertuani S, Angusti A, Manfredini S. The antioxidants and pro-antioxidants network: an overview. Curr Pharm Des. 2004; 10(14): 1677–1694.
- Rahman T, Hosen I, Islam MMT, et al. Oxidative stress and human health. Advances in Bioscience and Biotechnology. 2012; 03: 997–1019.
- Ray PD, Huang BW, Tsuji Y. Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cell Signal. 2012; 24(5): 981–990.
- Pravda J. Radical induction theory of ulcerative colitis. World J Gastroenterol. 2005; 11(16): 2371–2384.
- Theurl I, Aigner E, Theurl M, et al. Regulation of iron homeostasis in anemia of chronic disease and iron deficiency anemia: diagnostic and therapeutic implications. Blood. 2009; 113(21): 5277–5286.
- Pavlick KP, Laroux FS, Fuseler J, et al. Role of reactive metabolites of oxygen and nitrogen in inflammatory bowel disease. Free Radic Biol Med. 2002; 33(3): 311–322.
- Verspaget HW, Peña AS, Weterman IT, et al. Diminished neutrophil function in Crohn's disease and ulcerative colitis identified by decreased oxidative metabolism and low superoxide dismutase content. Gut. 1988; 29(2): 223–228.
- Ruan EA, Rao S, Burdick JS, et al. Glutathione levels in chronic inflammatory disorders of the human colon. Nutr Res. 1997; 17: 463–73.
- Hernanz A, Fernández-Vivancos E, Montiel C, et al. Changes in the intracellular homocysteine and glutathione content associated with aging. Life Sci. 2000; 67(11): 1317–1324.
- Bunker VW. Free radicals, antioxidants and ageing. Med Lab Sci. 1992; 49(4): 299–312.
- Beutler E. Red cell metabolism. [In:] A Manual of Biochemical Methods. Grune-Stratton. New York. ; 1971: 11–12.
- Flohé L, Günzler WA. Assays of glutathione peroxidase. Methods Enzymol. 1984; 105: 114–121.
- Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967; 70(1): 158–169.
- Placer ZA, Cushman LL, Johnson BC. Estimation of product of lipid peroxidation malondialdehyde in biochemical systems. Anals Biochem. 1966; 16: 359–64.
- Griess P. Bemerkungen zu der abhandlung der H.H. Weselsky und Benedikt “Ueber eininge azoverbindungen”. Chem Ber. 1879; 12: 426–28.
- Rana VS, Sharma S, Prasad KK, et al. Role of oxidative stress & antioxidant defence in ulcerative colitis patients form north India. Indian J Med Res. 2014; 139: 568–71.
- Mulder TP, Verspaget HW, Janssens AR, et al. Decrease in two intestinal copper/zinc containing proteins with antioxidant function in inflammatory bowel disease. Gut. 1991; 32(10): 1146–1150.
- Dröge W. Free radicals in the physiological control of cell function. Physiol Rev. 2002; 82(1): 47–95.
- Sido B, Hack V, Hochlehnert A, et al. Impairment of intestinal glutathione synthesis in patients with inflammatory bowel disease. Gut. 1998; 42(4): 485–492.
- Stadtman ER, Levine RL. Protein oxidation. Annals of the New York Academy of Sciences. 2000; 899: 191–208.
- Vaiopoulou A, Gazouli M, Papadopoulou A, et al. Serum protein profiling of adults and children with Crohn disease. J Pediatr Gastroenterol Nutr. 2015; 60(1): 42–47.
- Krzystek-Korpacka M, Neubauer K, Berdowska I, et al. Impaired erythrocyte antioxidant defense in active inflammatory bowel disease: impact of anemia and treatment. Inflamm Bowel Dis. 2010; 16(9): 1467–1475.
- Akman T, Akarsu M, Akpinar H, et al. Erythrocyte deformability and oxidative stress in inflammatory bowel disease. Dig Dis Sci. 2012; 57(2): 458–464.
- RAVIN HA. An improved colorimetric enzymatic assay of ceruloplasmin. J Lab Clin Med. 1961; 58: 161–168.
- Itoh K, Ishii T, Wakabayashi N, et al. Regulatory mechanism of cellular response to oxidative stress. Free Radic Biol Med. 1999; 31: 319–25.
- de Sousa CV, Sales MM, Rosa TS, et al. The Antioxidant Effect of Exercise: A Systematic Review and Meta-Analysis. Sports Med. 2017; 47(2): 277–293.
- Pippenger CE, Browne RW, Armstrong D. Regulatory antioxidant enzymes. [In:] Armstrong D (Ed.). Free radical and antioxidant protocols. Methods in molecular biology. Humana Press. Totowa, New Yersey. 1998; Vol. ; 108.
- Janero DR. Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radic Biol Med. 1990; 9(6): 515–540.
- Alzoghaibi MA, Al Mofleh IA, Al-Jebreen AM. Lipid peroxides in patients with inflammatory bowel disease. Saudi J Gastroenterol. 2007; 13(4): 187–190.
- Trougakos IP, Gonos ES. Regulation of clusterien/apolipoprotein J, a functional homologue to the small heat shock proteins, by oxidative stress in aging and age-related diseases. Free Radic Res. 2006; 40: 1335–38.
- O’Donnell VB, Chumley PH, Hogg N, et al. Nitric oxide inhibition of lipid peroxidation: kinetics of reaction with lipid peroxyl radicals and comparison with alphatocopherol, Biochemistry. 1997; 36: 15216–23.
- Opara EC Oxidative stress Dis Mon. 2006; 52: 183–98.
- Avdagić N, Zaćiragić A, Babić N, et al. Nitric oxide as a potential biomarker in inflammatory bowel disease. Bosn J Basic Med Sci. 2013; 13(1): 5–9.
- Tüzün A, Erdil A, Inal V, et al. Oxidative stress and antioxidant capacity in patients with inflammatory bowel disease. Clin Biochem. 2002; 35(7): 569–572.