Kinetic resolution of a b-adrenolytic drug with the use of lipases as enantioselective biocatalysts
Abstract
The study presented herein is focused on optimisation of the kinetic resolution of racemic propranolol by screening acetylating agent, reaction medium, and enantioselective biocatalysts. The effects of acetylating agent and reaction medium on the efficiency of performed enantioselective biotransformation were inves-tigated. Furthermore, the catalytic activities on nine commercially available lipases were tested. Finally, the composition of reaction medium was selected, based on previously obtained results, providing the most efficient process of kinetic resolution of racemic propranolol.
Keywords: enzyme(RS)-propranololkinetic resolutionenantioselective acetylationCandida rugosa (OF) lipase
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