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Effect of low level laser irradiation on VEGF gene expression in cultured endothelial cells
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Abstract
Background. Endothelial cells play a crucial role in the angiogenesis which is initiated by vascular endothelial growth factor (VEGF). Low level laser therapy (LLLT) stimulates repair processes which are based on the formation of new blood vessels.
Aim. The aim of this study was to evaluate the impact of LLLT on VEGF gene expression in endothelial cells cultured in vitro.
Material and methods. Freshly isolated endothelial cells from the human umbilical vein endothelial cells (HUVEC) line were used in the study. The cells were irradiated with a semiconductor laser emitting visiblelaser radiation at the wave length of 630 nm and the power of 30 mW, and radiation at the wavelength of 808 nm and the power of 60 mW in the infrared range. The study was performed with cell cultures subjected to four different procedures: I — control cells (not subjected to irradiation); II — cells subjected to an energy dose of 2 J/cm2; III — cells subjected to an energy dose of 4 J/cm2; and IV — cells subjected to an energy dose of 8 J/cm2. The cells were cultured for six days, and exposed to irradiation twice. The next step was to evaluate the VEGF gene expression by applying real-time PCR.
Results. By using low power laser irradiation, we obtained a statistically significant increase in VEGF gene expression, particularly at doses of 2 and 8 J/cm2 in the wave length range of 630 nm. The wave length of 808 nm had a similar effect on increases in gene expression. However, the differences were statistically non-significant when compared to the control cells.
Conclusions. This study has shown that low-power laser radiation in the visible light spectrum (630 nm) results in de novo formation of VEGF-A in the endothelial cells in culture.
Abstract
Background. Endothelial cells play a crucial role in the angiogenesis which is initiated by vascular endothelial growth factor (VEGF). Low level laser therapy (LLLT) stimulates repair processes which are based on the formation of new blood vessels.
Aim. The aim of this study was to evaluate the impact of LLLT on VEGF gene expression in endothelial cells cultured in vitro.
Material and methods. Freshly isolated endothelial cells from the human umbilical vein endothelial cells (HUVEC) line were used in the study. The cells were irradiated with a semiconductor laser emitting visiblelaser radiation at the wave length of 630 nm and the power of 30 mW, and radiation at the wavelength of 808 nm and the power of 60 mW in the infrared range. The study was performed with cell cultures subjected to four different procedures: I — control cells (not subjected to irradiation); II — cells subjected to an energy dose of 2 J/cm2; III — cells subjected to an energy dose of 4 J/cm2; and IV — cells subjected to an energy dose of 8 J/cm2. The cells were cultured for six days, and exposed to irradiation twice. The next step was to evaluate the VEGF gene expression by applying real-time PCR.
Results. By using low power laser irradiation, we obtained a statistically significant increase in VEGF gene expression, particularly at doses of 2 and 8 J/cm2 in the wave length range of 630 nm. The wave length of 808 nm had a similar effect on increases in gene expression. However, the differences were statistically non-significant when compared to the control cells.
Conclusions. This study has shown that low-power laser radiation in the visible light spectrum (630 nm) results in de novo formation of VEGF-A in the endothelial cells in culture.
Keywords
low level laser therapy, angiogenesis, VEGF gene
About this articleTitle
Effect of low level laser irradiation on VEGF gene expression in cultured endothelial cells
Journal
Issue
Article type
Original article
Pages
61-65
Published online
2014-09-11
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803
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1649
Bibliographic record
Folia Medica Copernicana 2014;2(2):61-65.
Keywords
low level laser therapy
angiogenesis
VEGF gene
Authors
Krzysztof Góralczyk
Justyna Szymańska
Katarzyna Linkowska
Barbara Ruszkowska-Ciastek
Łukasz Gryko
Andrzej Zając
Tomasz Grzybowski
Danuta Rość