Retinoic acid-induced ventricular non-compacted cardiomyopathy in mice

Abstract

Background: Precise tissue concentration of retinoic acid (RA) is indispensable for proper interaction of second heart fieldcells with cardiac neural crest cells and induction of signalling pathways important for normal myocardial growth.

Aim: Since RA deficiency during embryogenesis induces noncompaction, we hypothesised that excess RA at the stage of hearttube elongation may cause thinning of the myocardial wall which leads to noncompaction.

Methods: RA was administered at 70 mg/kg b.w. on 8.5 days post coitus (dpc) to pregnant mice to elicit cardiac malformationsin foetuses. We studied noncompaction development in RA-treated mouse offspring. The cardiac noncompaction was evaluatedin different stages of heart development as the quotient of the distance between the epicardial surface and trabecular tips(represented by a) and the distance between the epicardial surface and trabecular recesses (represented by b) in RA-treatedhearts compared to control non-treated.

Results: We demonstrated that apart from outflow tract defects such as double outlet right ventricle, transposition of the greatarteries and tetralogy of Fallot in foetuses in mouse offspring, noncompaction occurs in about 42% of cases. At the stage of13 dpc and later in development the ratio a/b was higher in RA-treated hearts exhibiting noncompaction compared to thecontrol hearts. This cardiomyopathy was more evident in the right ventricle than in the left ventricle.

Conclusions: Noncompaction caused by RA overdose can be elicited in part of the mouse offspring by administering RA atthe stage of cardiac tube elongation.