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open access

Vol 94, No 1 (2023)
Research paper
Published online: 2022-06-23
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Expression pattern of circadian rhythm-related genes and its potential relationship with miRNAs activity in endometrial cancer

Ewelina Hermyt1, Nikola Zmarzly2, Celina Kruszniewska-Rajs3, Joanna Gola3, Agnieszka Jeda-Golonka1, Katarzyna Szczepanek1, Urszula Mazurek4, Andrzej Witek1
DOI: 10.5603/GP.a2022.0063
·
Pubmed: 36748323
·
Ginekol Pol 2023;94(1):33-40.
Affiliations
  1. Department of Gynecology and Obstetrics, Faculty of Medical Sciences in Katowice, Medical University of Silesia in Katowice, Poland
  2. Department of Histology, Cytophysiology and Embryology, Faculty of Medicine in Zabrze, Academy of Silesia in Katowice, Zabrze, Poland, Poland
  3. Department of Molecular Biology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Sosnowiec, Poland
  4. Jozef Tyszkiewicz Higher School in Bielsko-Biala, Poland

open access

Vol 94, No 1 (2023)
ORIGINAL PAPERS Gynecology
Published online: 2022-06-23

Abstract

Objectives: The circadian clock is an autonomous oscillator that controls key aspects of cell physiology, including metabolism, transcriptional state, and cell signaling. Disturbances of circadian rhythms lead to disruption of cell and tissue homeostasis, which promotes carcinogenesis. The aim of the study was to determine the expression of circadian rhythm-related genes in endometrial cancer and to select miRNAs involved in the regulation of their expression. Material and methods: 50 endometrial tissue samples were collected from patients who underwent hysterectomy: 40 diagnosed with endometrial cancer and 10 without cancer. Expression profile of circadian rhythm-related genes was evaluated using microarrays and validated with RT-qPCR. MicroRNA expression was assessed using microarrays. Then mirTAR tool was used to identify miRNAs involved in the expression regulation of circadian rhythm-related genes. Results: CLOCK expression is disrupted in endometrial cancer, which may be due to miR-15b, miR-331-3p and miR-200a overexpression. Elevated NPAS2 and CSNK1D levels may be associated with miR-432 silencing. In addition, high miR-874 and miR-200a expression may be potentially responsible for the reduction of PER3 level. Conclusions: Change of CLOCK, CSNK1D, NPAS2 and PER3 expression may suggest that circadian rhythms are disrupted in endometrial cancer. A possible mechanism of the observed changes may be related to miRNAs activity.

Abstract

Objectives: The circadian clock is an autonomous oscillator that controls key aspects of cell physiology, including metabolism, transcriptional state, and cell signaling. Disturbances of circadian rhythms lead to disruption of cell and tissue homeostasis, which promotes carcinogenesis. The aim of the study was to determine the expression of circadian rhythm-related genes in endometrial cancer and to select miRNAs involved in the regulation of their expression. Material and methods: 50 endometrial tissue samples were collected from patients who underwent hysterectomy: 40 diagnosed with endometrial cancer and 10 without cancer. Expression profile of circadian rhythm-related genes was evaluated using microarrays and validated with RT-qPCR. MicroRNA expression was assessed using microarrays. Then mirTAR tool was used to identify miRNAs involved in the expression regulation of circadian rhythm-related genes. Results: CLOCK expression is disrupted in endometrial cancer, which may be due to miR-15b, miR-331-3p and miR-200a overexpression. Elevated NPAS2 and CSNK1D levels may be associated with miR-432 silencing. In addition, high miR-874 and miR-200a expression may be potentially responsible for the reduction of PER3 level. Conclusions: Change of CLOCK, CSNK1D, NPAS2 and PER3 expression may suggest that circadian rhythms are disrupted in endometrial cancer. A possible mechanism of the observed changes may be related to miRNAs activity.

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Keywords

endometrial cancer; circadian rhythms; miRNAs; microarrays

About this article
Title

Expression pattern of circadian rhythm-related genes and its potential relationship with miRNAs activity in endometrial cancer

Journal

Ginekologia Polska

Issue

Vol 94, No 1 (2023)

Article type

Research paper

Pages

33-40

Published online

2022-06-23

Page views

3275

Article views/downloads

646

DOI

10.5603/GP.a2022.0063

Pubmed

36748323

Bibliographic record

Ginekol Pol 2023;94(1):33-40.

Keywords

endometrial cancer
circadian rhythms
miRNAs
microarrays

Authors

Ewelina Hermyt
Nikola Zmarzly
Celina Kruszniewska-Rajs
Joanna Gola
Agnieszka Jeda-Golonka
Katarzyna Szczepanek
Urszula Mazurek
Andrzej Witek

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