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Published online: 2022-08-01
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LncRNA-loc391533 is involved in the progression of preeclampsia through VEGF

Jun Xiong1, Xuan Jin2, Kangxiang Xu3, Bingqi Wu3, Yuqi Xu3, Dong Ruan3, Xiaoju He1
Affiliations
  1. 2nd Affiliated Hospital of Nanchang University, Nanchang, China, China
  2. 1st Affiliated Hospital of Nanchang University, Nanchang, China, China
  3. Nanchang University, Nanchang, China, China

open access

Ahead of Print
ORIGINAL PAPERS Obstetrics
Published online: 2022-08-01

Abstract

Objectives: Preeclampsia (PE) is a leading cause of maternal death worldwide, which is one of the most major pregnancy complications. The effects of vascular endothelial growth factor (VEGF) and lncRNA-loc391533 on PE were evaluated in the present study.

Material and methods: Expression of VEGF in pregnant women with PE was determined using immunohistochemical and enzyme linked immunosorbent assay (ELISA). The effects of lncRNA-loc391533 knockdown and overexpression on VEGF expression was detected using quantitative polymerase chain reaction (qPCR) and western blotting. Loss/gain-of-function assays were performed to evaluate the role of lncRNA-loc391533 on proliferation, cell cycle and migration of trophoblasts HTR-8/SVneo cells.

Results: We found that VEGF and its receptor VEGFR1/2 were low expressed in PE. Knockdown of lncRNA-loc391533 enhanced VEGF expression, while overexpression of lncRNA-loc391533 downregulated VEGF. Moreover, lncRNA-loc391533 was required for proliferation and migration of HTR-8/SVneo cells.

Conclusions: In conclusion, our findings emphasized that lncRNA-loc391533 exhibited a critical role in progression of PE through VEGF, which might as a novel therapeutic target for PE treatment.

Abstract

Objectives: Preeclampsia (PE) is a leading cause of maternal death worldwide, which is one of the most major pregnancy complications. The effects of vascular endothelial growth factor (VEGF) and lncRNA-loc391533 on PE were evaluated in the present study.

Material and methods: Expression of VEGF in pregnant women with PE was determined using immunohistochemical and enzyme linked immunosorbent assay (ELISA). The effects of lncRNA-loc391533 knockdown and overexpression on VEGF expression was detected using quantitative polymerase chain reaction (qPCR) and western blotting. Loss/gain-of-function assays were performed to evaluate the role of lncRNA-loc391533 on proliferation, cell cycle and migration of trophoblasts HTR-8/SVneo cells.

Results: We found that VEGF and its receptor VEGFR1/2 were low expressed in PE. Knockdown of lncRNA-loc391533 enhanced VEGF expression, while overexpression of lncRNA-loc391533 downregulated VEGF. Moreover, lncRNA-loc391533 was required for proliferation and migration of HTR-8/SVneo cells.

Conclusions: In conclusion, our findings emphasized that lncRNA-loc391533 exhibited a critical role in progression of PE through VEGF, which might as a novel therapeutic target for PE treatment.

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Keywords

preeclampsia; VEGF; lncRNA-loc391533; proliferation; migration

About this article
Title

LncRNA-loc391533 is involved in the progression of preeclampsia through VEGF

Journal

Ginekologia Polska

Issue

Ahead of Print

Article type

Research paper

Published online

2022-08-01

Page views

536

Article views/downloads

350

DOI

10.5603/GP.a2022.0056

Pubmed

35942721

Keywords

preeclampsia
VEGF
lncRNA-loc391533
proliferation
migration

Authors

Jun Xiong
Xuan Jin
Kangxiang Xu
Bingqi Wu
Yuqi Xu
Dong Ruan
Xiaoju He

References (30)
  1. American College of Obstetricians and Bulletins-Obstetrics Gynecologists Committee on Practice 2020. Gestational Hypertension and Preeclampsia: ACOG Practice Bulletin, Number 222. Obstet Gynecol. 2020; 135(6): e237–e260.
  2. Tranquilli AL, Dekker G, Magee L, et al. The classification, diagnosis and management of the hypertensive disorders of pregnancy: A revised statement from the ISSHP. Pregnancy Hypertens. 2014; 4(2): 97–104.
  3. Lisonkova S, Sabr Y, Mayer M, et al. Maternal morbidity associated with early-onset and late-onset preeclampsia. Obstet Gynecol. 2014; 124(4): 771–781.
  4. Tomimatsu T, Mimura K, Matsuzaki S. Preeclampsia: maternal systemic vascular disorder caused by generalized endothelial dysfunction due to placental antiangiogenic factors. Int J Mol Sci. 2019; 20(17): 4246.
  5. Cox AG, Marshall SA, Palmer KR, et al. Current and emerging pharmacotherapy for emergency management of preeclampsia. Expert Opin Pharmacother. 2019; 20(6): 701–712.
  6. Birney E, Stamatoyannopoulos JA, Dutta A, et al. Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project. Nature. 2007; 447(7146): 799–816.
  7. Kapranov P, Cheng J, Dike S, et al. RNA maps reveal new RNA classes and a possible function for pervasive transcription. Science. 2007; 316(5830): 1484–1488.
  8. Rinn JL, Kertesz M, et al. Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs. Cell. 2007; 129(7): 1311–1323.
  9. Chen L, Dzakah EE, Shan G. Targetable long non-coding RNAs in cancer treatments. Cancer Lett. 2018; 418: 119–124.
  10. Luo Q, Chen Y. Long noncoding RNAs and Alzheimer's disease. Clin Interv Aging. 2016; 11: 867–872.
  11. Chanda K, Das S, Chakraborty J, et al. Altered levels of long NcRNAs meg3 and neat1 in cell and animal models of Huntington's disease. RNA Biol. 2018; 15(10): 1348–1363.
  12. Huang Y. The novel regulatory role of lncRNA-miRNA-mRNA axis in cardiovascular diseases. J Cell Mol Med. 2018; 22(12): 5768–5775.
  13. Sui W, Lin H, Peng W, et al. Molecular dysfunctions in acute rejection after renal transplantation revealed by integrated analysis of transcription factor, microRNA and long noncoding RNA. Genomics. 2013; 102(4): 310–322.
  14. Song X, Luo X, Gao Q, et al. Dysregulation of lncRNAs in placenta and pathogenesis of preeclampsia. Curr Drug Targets. 2017; 18(10): 1165–1170.
  15. Wu JL, Wang YG, Gao GM. Overexpression of lncRNA TCL6 promotes preeclampsia progression by regulating PTEN. Eur Rev Med Pharmacol Sci. 2019; 23(10): 4066–4072.
  16. Haonon O, Rucksaken R, Pinlaor P, et al. Upregulation of 14-3-3 eta in chronic liver fluke infection is a potential diagnostic marker of cholangiocarcinoma. Proteomics Clin Appl. 2016; 10(3): 248–256.
  17. Liu Z, Li J, Chen J, et al. MCM family in HCC: MCM6 indicates adverse tumor features and poor outcomes and promotes S/G2 cell cycle progression . BMC Cancer. 2018; 18(1): 200.
  18. Konstantinou GN. Enzyme-Linked Immunosorbent Assay (ELISA). Methods Mol Biol. 2017; 1592: 79–94.
  19. Trapiella-Alfonso L, Alexandre L, Fraichard C, et al. VEGF (vascular endothelial growth factor) functionalized magnetic beads in a microfluidic device to improve the angiogenic balance in preeclampsia . Hypertension. 2019; 74(1): 145–153.
  20. Role of dyslipidemia in preeclampsia: A review of lipidomic analysis of blood, placenta, syncytiotrophoblast microvesicles and umbilical cord artery from women with preeclampsia . Prostaglandins Other Lipid Mediat. 2018; 139: 19–23.
  21. Rana S, Lemoine E, Granger JP, et al. Preeclampsia. Circ Res. 2019; 124(7): 1094–1112.
  22. ACOG Committee Opinion No. 743: Low-dose aspirin use during pregnancy. Obstet Gynecol. 2018; 132(1): e44–e52.
  23. Thadhani R, Hagmann H, Schaarschmidt W, et al. removal of soluble fms-like tyrosine kinase-1 by dextran sulfate apheresis in preeclampsia . J Am Soc Nephrol. 2016; 27(3): 903–913.
  24. Leanos-Miranda A, Campos-Galicia I, Berumen-Lechuga MG, et al. circulating angiogenic factors and the risk of preeclampsia in systemic lupus erythematosus pregnancies . J Rheumatol. 2015; 42(7): 1141–1149.
  25. Xiao Z, Li S, Yu Y, et al. VEGF-A regulates sFlt-1 production in trophoblasts through both Flt-1 and KDR receptors. Mol Cell Biochem. 2018; 449(1–2): 1–8.
  26. Alahakoon TI, Medbury H, Williams H, et al. Maternal Flt-1 and endoglin expression by circulating monocyte subtype and polarization in preeclampsia and fetal growth restriction. Eur J Obstet Gynecol Reprod Biol X. 2019; 3: 100024.
  27. Hodel M, Blank PR, Marty P. sFlt-1/PlGF ratio as a predictive marker in women with suspected preeclampsia: an economic evaluation from a Swiss perspective. Dis Markers. 2019; 2019: 4096847.
  28. Zou Y, Li Q, Xu Y, et al. Promotion of trophoblast invasion by lncRNA MVIH through inducing Jun-B . J Cell Mol Med. 2018; 22(2): 1214–1223.
  29. Liu X, Chen H, Kong W, et al. Down-regulated long non-coding RNA-ATB in preeclampsia and its effect on suppressing migration, proliferation, and tube formation of trophoblast cells . Placenta. 2017; 49: 80–87.
  30. Li Q, Zhang J, Su DM. lncRNA TUG1 modulates proliferation, apoptosis, invasion, and angiogenesis via targeting miR-29b in trophoblast cells . Hum Genomics. 2019; 13(1): 50.

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