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Published online: 2021-11-18
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The relationship between hsa_circ_0051326 and HLA-G expression in the blood of patients with pre-eclampsia

Li Wang1, Xue Wang2, Xiaoju Chen1, Dongcai Wu1, Hui Cen1, Dongrui Mao1, Yuqiao Mo1, Linmei Zheng1
DOI: 10.5603/GP.a2021.0194
Affiliations
  1. Department of Obstetrics, Hainan General Hospital, Hainan Affiliated hospital of Hainan medical university, Haikou, China
  2. Department of internal emergency medicine, Hainan General Hospital, Hainan Affiliated hospital of Hainan medical university, Haikou, China

open access

Ahead of Print
ORIGINAL PAPERS Obstetrics
Published online: 2021-11-18

Abstract

Objectives: To investigate the relationship between hsa_circ_0051326 and HLA-G expression in the blood of patients with pre-eclampsia.

Material and methods: Real-time PCR (qRT-PCR) was used to detect the hsa_circ_0051326 expression level. Enzyme-linked immunosorbent assay (ELISA), qRT-PCR, and western blotting were used to detect HLA-G expression in blood in 50 patients with pre-eclampsia and 50 normal pregnant women.

Results: HLA-G protein expression level was decreased significantly in the blood of patients with pre-eclampsia. hsa_circ_0051326 and HLA-G mRNA in blood were decreased significantly in the pre-eclampsia patients compared with normal pregnant women. There was a positive correlation between the expression of serum hsa_circ_0051326 with HLA-G mRNA.

Conclusions: Serum hsa_circ_0051326 was a new diagnostic biomarker for pre-eclampsia with equivalent efficiency of HLA-G. Maternal serum hsa_circ_0051326 level may pre-diagnose potentially pre-eclampsia before delivery.

Abstract

Objectives: To investigate the relationship between hsa_circ_0051326 and HLA-G expression in the blood of patients with pre-eclampsia.

Material and methods: Real-time PCR (qRT-PCR) was used to detect the hsa_circ_0051326 expression level. Enzyme-linked immunosorbent assay (ELISA), qRT-PCR, and western blotting were used to detect HLA-G expression in blood in 50 patients with pre-eclampsia and 50 normal pregnant women.

Results: HLA-G protein expression level was decreased significantly in the blood of patients with pre-eclampsia. hsa_circ_0051326 and HLA-G mRNA in blood were decreased significantly in the pre-eclampsia patients compared with normal pregnant women. There was a positive correlation between the expression of serum hsa_circ_0051326 with HLA-G mRNA.

Conclusions: Serum hsa_circ_0051326 was a new diagnostic biomarker for pre-eclampsia with equivalent efficiency of HLA-G. Maternal serum hsa_circ_0051326 level may pre-diagnose potentially pre-eclampsia before delivery.

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Keywords

pre-eclampsia; Human leukocyte antigen-G; hsa_circ_0051326; serum

About this article
Title

The relationship between hsa_circ_0051326 and HLA-G expression in the blood of patients with pre-eclampsia

Journal

Ginekologia Polska

Issue

Ahead of Print

Article type

Research paper

Published online

2021-11-18

DOI

10.5603/GP.a2021.0194

Keywords

pre-eclampsia
Human leukocyte antigen-G
hsa_circ_0051326
serum

Authors

Li Wang
Xue Wang
Xiaoju Chen
Dongcai Wu
Hui Cen
Dongrui Mao
Yuqiao Mo
Linmei Zheng

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