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Human papillomavirus genotyping in low-grade squamous intraepithelial lesions
, 13, 4, 4, 4, 4, 15
Affiliations- Department of Obstetrics and Gynecology, District Public Hospital in Poznan, Poland
- Marcin Przybylski M.D. Gynecology Specialised Medical Practice Poznan, Poland
- Poznan University of Medical Science, Poland
- Jagiellonian University Collegium Medicum, Department of Gynecology and Obstetrics, Cracow, Poland
- Dominik Pruski M.D. Gynecology Specialised Medical Practice Poznan, Poland
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Abstract
Objectives: Human papillomavirus infection is one of the most common sexually transmitted infections. Histological LSIL in 70–80% of cases will regress spontaneously, while a subset is associated with residual risk for a future precancerous lesion. This study evaluates the performance of HPV genotypes for LSIL preceded by normal or mildly abnormal Pap smear. Material and methods: We provide a prospective observational cohort study. We obtained material from 428 women registered to Specialist Medical Practice and Provincial Hospital in Poznań in 2018–2021. In the current study, we analyze results from the first 112 inclusions with the diagnosis of LSIL from a cervical biopsy. The probe for the molecular test was collected with a combi brush and passed to the independent, standardized laboratory. HPV detection was done using PCR followed by DNA enzyme immunoassay and genotyping with a reverse hybridization line probe assay. Sequence analysis was performed to characterize HPV — positive samples with unknown HPV genotypes. The molecular test detected DNA of 42 HPV genotypes. We performed statistical analyzes using the STATISTICA package 13.3. Results: We found that 77.7% of patients received HPV-positive test results. The most frequent HPV genotype was 16, which was assumed for 22.3%. We detected that following HPV types are next most common: HPV 56 (11.6%), HPV 52 (8.9%), HPV 31 (8.0%) and HPV 51 (8.0%). Among HPV 16-negative women, the vast majority are those living in the town (p = 0.048). Moreover, thyroid diseases were the most common comorbidities. Conclusions: To our knowledge, this study is the most extensive assessment of HPV genotypes in LSIL diagnoses in Poland.
Abstract
Objectives: Human papillomavirus infection is one of the most common sexually transmitted infections. Histological LSIL in 70–80% of cases will regress spontaneously, while a subset is associated with residual risk for a future precancerous lesion. This study evaluates the performance of HPV genotypes for LSIL preceded by normal or mildly abnormal Pap smear. Material and methods: We provide a prospective observational cohort study. We obtained material from 428 women registered to Specialist Medical Practice and Provincial Hospital in Poznań in 2018–2021. In the current study, we analyze results from the first 112 inclusions with the diagnosis of LSIL from a cervical biopsy. The probe for the molecular test was collected with a combi brush and passed to the independent, standardized laboratory. HPV detection was done using PCR followed by DNA enzyme immunoassay and genotyping with a reverse hybridization line probe assay. Sequence analysis was performed to characterize HPV — positive samples with unknown HPV genotypes. The molecular test detected DNA of 42 HPV genotypes. We performed statistical analyzes using the STATISTICA package 13.3. Results: We found that 77.7% of patients received HPV-positive test results. The most frequent HPV genotype was 16, which was assumed for 22.3%. We detected that following HPV types are next most common: HPV 56 (11.6%), HPV 52 (8.9%), HPV 31 (8.0%) and HPV 51 (8.0%). Among HPV 16-negative women, the vast majority are those living in the town (p = 0.048). Moreover, thyroid diseases were the most common comorbidities. Conclusions: To our knowledge, this study is the most extensive assessment of HPV genotypes in LSIL diagnoses in Poland.
Keywords
HPV genotyping; LSIL; low-grade lesion; cervix biopsy
About this articleTitle
Human papillomavirus genotyping in low-grade squamous intraepithelial lesions
Journal
Issue
Article type
Research paper
Pages
11-17
Published online
2021-10-05
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5847
Article views/downloads
632
DOI
Pubmed
Bibliographic record
Ginekol Pol 2022;93(1):11-17.
Keywords
HPV genotyping
LSIL
low-grade lesion
cervix biopsy
Authors
Marcin Przybylski
Sonja Millert-Kalinska
Andrzej Zmaczynski
Rafal Baran
Lucja Zaborowska
Robert Jach
Dominik Pruski
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