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Research paper
Published online: 2021-09-14
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Genotyping of human papillomavirus DNA in Wielkopolska region

Marcin Przybylski1, Dominik Pruski1, Sonja Millert-Kalinska1, Radoslaw Madry2, Malgorzata Lagiedo-Zelazowska3, Jan Sikora3, Andrzej Zmaczynski4, Rafal Baran4, Hanna Twardowska4, Anna Horbaczewska4, Robert Jach4
DOI: 10.5603/GP.a2021.0165
·
Pubmed: 34541643
Affiliations
  1. Department of Obstetrics and Gynecology, District Public Hospital in Poznan
  2. Department of Oncology, Poznan University of Medical Sciences, Poland
  3. Department of Immunology, Chair of Patomorphology and Clinical Immunology, Poznan University of Medical Sciences, Poland
  4. Department of Gynaecological Endocrinology and Gynaecology, Jagiellonian University Medical College, Cracow, Poland

open access

Ahead of Print
ORIGINAL PAPERS Gynecology
Published online: 2021-09-14

Abstract

Objectives: Human papillomavirus infection is one of the most common sexually transmitted diseases. Long-term exposure to the HPV leads to development of high-grade squamous intraepithelial lesions that can eventually transform into cervical cancer.

The aim of the study was to assess the HPV genotype distribution in patients with abnormal pap smear and provide prospective study.

Material and methods: We obtained material from 674 women who registered to Specialist Medical Practice in the years 2008–2020. The sample for the molecular test was collected using combi brush and forwarded to the independent, standardized laboratory. HPV detection was done using PCR followed by DNA enzyme immunoassay and reverse hybridization line probe assay for virus genotyping. Sequence analysis was performed to characterize virus genotypes in HPV — positive samples.

Results: We found that 53% of patients tested positive for HPV. The percentage decreased with age. The following HPV types were the most common: HPV — 16 (24.5%), HPV — 53 (13.1%), HPV — 31 (10.3%), HPV — 51 (9.7%), HPV — 56 (9.5%). To our knowledge, this study is the largest assessment of HPV genotypes in Poland.

Conclusions: Our results suggest that type-specific, high–risk HPV DNA — based screening should focus on HPV types 16, 31, 51, 56.

Abstract

Objectives: Human papillomavirus infection is one of the most common sexually transmitted diseases. Long-term exposure to the HPV leads to development of high-grade squamous intraepithelial lesions that can eventually transform into cervical cancer.

The aim of the study was to assess the HPV genotype distribution in patients with abnormal pap smear and provide prospective study.

Material and methods: We obtained material from 674 women who registered to Specialist Medical Practice in the years 2008–2020. The sample for the molecular test was collected using combi brush and forwarded to the independent, standardized laboratory. HPV detection was done using PCR followed by DNA enzyme immunoassay and reverse hybridization line probe assay for virus genotyping. Sequence analysis was performed to characterize virus genotypes in HPV — positive samples.

Results: We found that 53% of patients tested positive for HPV. The percentage decreased with age. The following HPV types were the most common: HPV — 16 (24.5%), HPV — 53 (13.1%), HPV — 31 (10.3%), HPV — 51 (9.7%), HPV — 56 (9.5%). To our knowledge, this study is the largest assessment of HPV genotypes in Poland.

Conclusions: Our results suggest that type-specific, high–risk HPV DNA — based screening should focus on HPV types 16, 31, 51, 56.

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Keywords

HPV; HPV genotypes; HPV screening; cervical cancer

About this article
Title

Genotyping of human papillomavirus DNA in Wielkopolska region

Journal

Ginekologia Polska

Issue

Ahead of Print

Article type

Research paper

Published online

2021-09-14

DOI

10.5603/GP.a2021.0165

Pubmed

34541643

Keywords

HPV
HPV genotypes
HPV screening
cervical cancer

Authors

Marcin Przybylski
Dominik Pruski
Sonja Millert-Kalinska
Radoslaw Madry
Malgorzata Lagiedo-Zelazowska
Jan Sikora
Andrzej Zmaczynski
Rafal Baran
Hanna Twardowska
Anna Horbaczewska
Robert Jach

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