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Long non-coding RNA H19 correlates with unfavorable prognosis and promotes cell migration and invasion in ovarian cancer
Affiliations- Hwamei Hospital, University of Chinese Academy of Sciences, Ning Bo, China, China
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Abstract
Objectives: The purpose of this study is to investigate the expression pattern of lncRNA H19 in OC tissues and to detect
the ability of H19 to influence OC cell migration and invasion in vitro.
Material and methods: We quantified the levels of H19 within the obtained cancerous and adjacent noncancerous tissues
from 258 OC patients. H19 association with patient progression-free survival (PFS) was analyzed by a Kaplan-Meier
plot. Expression levels of H19 were reduced by small interfering RNA transfection against H19 or restored by a H19 overexpression
plasmid transfection in OC cells. H19 effects on OC cell migration and invasion in vitro were evaluated using
wound-healing assay and transwell invasion assay. Wound healing assay and transwell invasion assay were used to
evaluate the effects of H19 on OC cell migration and invasion in vitro.
Results: H19 is upregulated remarkably in primary OC tissues and human OC cell lines (OVCAR3, SKOV3, A2780, and Caov-3).
We found that the median PFS was longer in patients with lower levels of H19 than in those with high levels, suggesting
that overexpres- sion of H19 was linked to poor prognosis in OC patients. Intriguingly, the depletion of H19 expression
induced by small interfering RNA inhibited the capability of migration and invasion of OC cell lines. Restoration of H19 in
OC cell lines significantly increased cell migration and invasion.
Conclusions: The key finding of the present study suggests that overexpression of H19 may be associated with an unfavorable
prognosis for OC and is likely to be a possible contributory force involved in OC cell migration and invasion.
H19 may provide a new and attractive target for future prognostic and therapeutic intervention of OC patients.
Abstract
Objectives: The purpose of this study is to investigate the expression pattern of lncRNA H19 in OC tissues and to detect
the ability of H19 to influence OC cell migration and invasion in vitro.
Material and methods: We quantified the levels of H19 within the obtained cancerous and adjacent noncancerous tissues
from 258 OC patients. H19 association with patient progression-free survival (PFS) was analyzed by a Kaplan-Meier
plot. Expression levels of H19 were reduced by small interfering RNA transfection against H19 or restored by a H19 overexpression
plasmid transfection in OC cells. H19 effects on OC cell migration and invasion in vitro were evaluated using
wound-healing assay and transwell invasion assay. Wound healing assay and transwell invasion assay were used to
evaluate the effects of H19 on OC cell migration and invasion in vitro.
Results: H19 is upregulated remarkably in primary OC tissues and human OC cell lines (OVCAR3, SKOV3, A2780, and Caov-3).
We found that the median PFS was longer in patients with lower levels of H19 than in those with high levels, suggesting
that overexpres- sion of H19 was linked to poor prognosis in OC patients. Intriguingly, the depletion of H19 expression
induced by small interfering RNA inhibited the capability of migration and invasion of OC cell lines. Restoration of H19 in
OC cell lines significantly increased cell migration and invasion.
Conclusions: The key finding of the present study suggests that overexpression of H19 may be associated with an unfavorable
prognosis for OC and is likely to be a possible contributory force involved in OC cell migration and invasion.
H19 may provide a new and attractive target for future prognostic and therapeutic intervention of OC patients.
Keywords
ovarian cancer; long non-coding RNA; H19; prognosis; migration
About this articleTitle
Long non-coding RNA H19 correlates with unfavorable prognosis and promotes cell migration and invasion in ovarian cancer
Journal
Issue
Article type
Research paper
Pages
1-6
Published online
2021-10-06
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5662
Article views/downloads
561
DOI
Pubmed
Bibliographic record
Ginekol Pol 2022;93(1):1-6.
Keywords
ovarian cancer
long non-coding RNA
H19
prognosis
migration
Authors
Hainong Ma
Li Gao
Huimin Yu
Xu Song
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