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Amniotic fluid NF-kB concentration in pregnant women with a high risk of prenatal screening test results
Affiliations- Kafkas University School of Medicine, Kars, Türkiye
- Ataturk University, School of Medicine, Erzurum, Turkey.
open access
Abstract
Objectives: Nuclear factor-kappa beta (NF-kB) can potentially be related to certain fetal chromosomal abnormalities. This study aimed to determine whether the concentration of NF-kB changes in the amniotic fluid (AF) of pregnant women who have a high risk of fetal down syndrome (DS) results in prenatal screening and diagnosis testing.
Material and methods: 108 patients with an abnormal first trimester combined screening test (FTCST) were subjected to amniocentesis and fetal karyotype analysis between 16 and 20 weeks of gestation. Amniocentesis material obtained from 86 patients conformed with our research criteria and only this was included in the study. Among the 86 amniocentesis results, there were 12 patients with confirmed DS. The karyotypes of the remaining patients were normal. Therefore the total study group was divided into two groups: patients with DS fetal karyotype (Group 1, n = 12) and patients with normal fetal karyotype (Group 2, n = 74). We used the ELISA method to assess the concentration of NF-kB and high sensitivity C-reactive protein (hsCRP) in each sample of AF.
Results: We observed significantly lower NF-kB concentrations in the AF of the women in Group 1 compared with the women in Group 2. Patients in Group 1 also had a higher concentration of hsCRP in their AF when compared with patients in Group 2. The FTCST results for patients in Group 1 showed a significantly higher risk than for those of Group 2. There were no statistically significant correlations detected when comparing the amniotic fluid nuclear factor-kappa beta (AF-NF-kB) levels with other clinical and laboratory parameters.
Conclusions: AF-NF-kB may play a role in the pathogenesis of fetal down syndrome.
Abstract
Objectives: Nuclear factor-kappa beta (NF-kB) can potentially be related to certain fetal chromosomal abnormalities. This study aimed to determine whether the concentration of NF-kB changes in the amniotic fluid (AF) of pregnant women who have a high risk of fetal down syndrome (DS) results in prenatal screening and diagnosis testing.
Material and methods: 108 patients with an abnormal first trimester combined screening test (FTCST) were subjected to amniocentesis and fetal karyotype analysis between 16 and 20 weeks of gestation. Amniocentesis material obtained from 86 patients conformed with our research criteria and only this was included in the study. Among the 86 amniocentesis results, there were 12 patients with confirmed DS. The karyotypes of the remaining patients were normal. Therefore the total study group was divided into two groups: patients with DS fetal karyotype (Group 1, n = 12) and patients with normal fetal karyotype (Group 2, n = 74). We used the ELISA method to assess the concentration of NF-kB and high sensitivity C-reactive protein (hsCRP) in each sample of AF.
Results: We observed significantly lower NF-kB concentrations in the AF of the women in Group 1 compared with the women in Group 2. Patients in Group 1 also had a higher concentration of hsCRP in their AF when compared with patients in Group 2. The FTCST results for patients in Group 1 showed a significantly higher risk than for those of Group 2. There were no statistically significant correlations detected when comparing the amniotic fluid nuclear factor-kappa beta (AF-NF-kB) levels with other clinical and laboratory parameters.
Conclusions: AF-NF-kB may play a role in the pathogenesis of fetal down syndrome.
Keywords
down syndrome; nuclear factor-kappa beta; amniotic fluid; prenatal screening test
About this articleTitle
Amniotic fluid NF-kB concentration in pregnant women with a high risk of prenatal screening test results
Journal
Issue
Article type
Research paper
Pages
577-580
Published online
2018-10-31
Page views
1872
Article views/downloads
1224
DOI
Pubmed
Bibliographic record
Ginekol Pol 2018;89(10):577-580.
Keywords
down syndrome
nuclear factor-kappa beta
amniotic fluid
prenatal screening test
Authors
Haldun Arpacı
Omer Erkan Yapca
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