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Expression profiling of genes modulated by rosmarinic acid (RA) in MCF-7 breast cancer cells
Affiliations- Department of Stem Cells and Regenerative Medicine, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Poznan, Poland
- Clinic of Pediatric Orthopedics, Pomeranian Medical University, Szczecin, Poland
- Department of Pharmacology and Pharmacoeconomics, Pomeranian Medical University, Szczecin, Poland
- Division of Perinatology and Women’s Diseases, Poznan University of Medical Sciences, Poznan, Poland
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Abstract
Objectives: Cancer is the second most common cause of death, with breast cancer (BC) as the most frequently diagnosed neoplasm among females. The origin of BC is multifactorial and depends on environmental and genetic factors. The disease presents a significant challenge due to its drug resistance and frequent metastasis. Thus, new effective therapies and metastasis prevention are much needed. Rosmarinic acid (RA) is a natural polyphenol which possesses the ability to inhibit BC cell proliferation and demonstrates cytotoxic properties against those cells. In our study, we examined the effect of RA on the expression of ZEB1, MDM2, ABCB1, PTEN and TWIST1 genes in MCF-7 breast cancer cells.
Material and methods: MCF-7 cell cultures were treated with 0.2 μM doxorubicin (DOX) and 1.5, 15 or 50 μM of RA. Real-time PCR reaction was performed to analyze gene expression levels.
Results: PCR analysis showed a significant increase of the ZEB1 gene expression, which was about 3-fold for DOX 0.2 μM, 9-fold for 0.2 μM DOX + 1.5 μM RA and 0.2 μM DOX + 15 μM RA (p < 0.05), and about 6.5-fold for 0.2 μM DOX + 50 μM RA (p < 0.05). Furthermore, a decrease of the MDM2 gene expression was observed in all of the examined variants and was about 40–75% (p < 0.05). No influence of DOX and RA combined with DOX on the ABCB1, TWIST1 and PTEN genes was found.
Conclusions: The results of our study suggest that RA might be used as an adjuvant therapeutic factor in BC treatment.
Abstract
Objectives: Cancer is the second most common cause of death, with breast cancer (BC) as the most frequently diagnosed neoplasm among females. The origin of BC is multifactorial and depends on environmental and genetic factors. The disease presents a significant challenge due to its drug resistance and frequent metastasis. Thus, new effective therapies and metastasis prevention are much needed. Rosmarinic acid (RA) is a natural polyphenol which possesses the ability to inhibit BC cell proliferation and demonstrates cytotoxic properties against those cells. In our study, we examined the effect of RA on the expression of ZEB1, MDM2, ABCB1, PTEN and TWIST1 genes in MCF-7 breast cancer cells.
Material and methods: MCF-7 cell cultures were treated with 0.2 μM doxorubicin (DOX) and 1.5, 15 or 50 μM of RA. Real-time PCR reaction was performed to analyze gene expression levels.
Results: PCR analysis showed a significant increase of the ZEB1 gene expression, which was about 3-fold for DOX 0.2 μM, 9-fold for 0.2 μM DOX + 1.5 μM RA and 0.2 μM DOX + 15 μM RA (p < 0.05), and about 6.5-fold for 0.2 μM DOX + 50 μM RA (p < 0.05). Furthermore, a decrease of the MDM2 gene expression was observed in all of the examined variants and was about 40–75% (p < 0.05). No influence of DOX and RA combined with DOX on the ABCB1, TWIST1 and PTEN genes was found.
Conclusions: The results of our study suggest that RA might be used as an adjuvant therapeutic factor in BC treatment.
Keywords
breast cancer; rosmarinic acid; molecular study; expression; adjuvant therapy
About this articleTitle
Expression profiling of genes modulated by rosmarinic acid (RA) in MCF-7 breast cancer cells
Journal
Issue
Article type
Research paper
Pages
541-545
Published online
2018-10-31
Page views
1804
Article views/downloads
1436
DOI
Pubmed
Bibliographic record
Ginekol Pol 2018;89(10):541-545.
Keywords
breast cancer
rosmarinic acid
molecular study
expression
adjuvant therapy
Authors
Bogna Juskowiak
Anna Bogacz
Marlena Wolek
Adam Kamiński
Izabela Uzar
Agnieszka Seremak-Mrozikiewicz
Bogusław Czerny
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