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Vol 89, No 5 (2018)
Research paper
Published online: 2018-05-30
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Placental expression of AChE, α7nAChR and NF-κB in patients with preeclampsia

Linmei Zheng1, Lei Shi1, Zhongyi Zhou1, Xiaoju Chen1, Li Wang1, Zhicheng Lu1, Rong Tang1
DOI: 10.5603/GP.a2018.0043
·
Pubmed: 30084476
·
Ginekol Pol 2018;89(5):249-255.
Affiliations
  1. Department of General Surgery, Hainan Provincial People's Hospital, HuaXiu Road, Haikou, Hainan, 570100 Haikou, Hainan, China

open access

Vol 89, No 5 (2018)
ORIGINAL PAPERS Obstetrics
Published online: 2018-05-30

Abstract

Objectives: This study aimed to investigate placental expression of AChE, α7nAChR and NF-κB in patients with preeclampsia and discuss about its clinical significance.

Material and methods: mRNA expression levels of acetylcholine (AChE), alpha-7 nicotinic acetylcholine receptor (α7nAChR) and nuclear factor-kB (NF-κB) in placenta were detected by qRT-PCR, and protein levels were determined by immunohis­tological analysis and Western Blot in 35 women with preeclampsia (including 20 cases of mild preeclampsia and 15 cases of severe preeclampsia) and 30 cases in control group, respectively.

Results: The expression of AChE mRNA and protein in placenta increased significantly in patients with preeclampsia compared with the control group (p < 0.01). It was lower in patients with severe preeclampsia than in patients with mild preeclampsia (p < 0.05). The expression of α7nAChR mRNA and protein in placenta decreased significantly in patients with preeclampsia compared with the control group (p < 0.01). However, the expression of α7nAChR mRNA and protein in patients with severe preeclampsia was higher than that in patients with mild preeclampsia, without significant difference(p > 0.05). The expression of NF-κB protein in placenta decreased significantly in patients with preeclampsia compared with the control group(p < 0.01). It was higher in patients with severe preeclampsia than in patients with mild preeclampsia (p < 0.05), but there was no significant difference between preeclampsia group and control group in the expression of NF-κB mRNA in placenta (p > 0.05). The results of Western blotting assay were consistent with those of immunohistochemistry.

Conclusions: Abnormal expression of AChE, α7nAChR and NF-κB in placenta may be associated with preeclampsia. Cho­linergic anti-inflammatory pathway may play an important role in the pathogenesis of preeclampsia.

Abstract

Objectives: This study aimed to investigate placental expression of AChE, α7nAChR and NF-κB in patients with preeclampsia and discuss about its clinical significance.

Material and methods: mRNA expression levels of acetylcholine (AChE), alpha-7 nicotinic acetylcholine receptor (α7nAChR) and nuclear factor-kB (NF-κB) in placenta were detected by qRT-PCR, and protein levels were determined by immunohis­tological analysis and Western Blot in 35 women with preeclampsia (including 20 cases of mild preeclampsia and 15 cases of severe preeclampsia) and 30 cases in control group, respectively.

Results: The expression of AChE mRNA and protein in placenta increased significantly in patients with preeclampsia compared with the control group (p < 0.01). It was lower in patients with severe preeclampsia than in patients with mild preeclampsia (p < 0.05). The expression of α7nAChR mRNA and protein in placenta decreased significantly in patients with preeclampsia compared with the control group (p < 0.01). However, the expression of α7nAChR mRNA and protein in patients with severe preeclampsia was higher than that in patients with mild preeclampsia, without significant difference(p > 0.05). The expression of NF-κB protein in placenta decreased significantly in patients with preeclampsia compared with the control group(p < 0.01). It was higher in patients with severe preeclampsia than in patients with mild preeclampsia (p < 0.05), but there was no significant difference between preeclampsia group and control group in the expression of NF-κB mRNA in placenta (p > 0.05). The results of Western blotting assay were consistent with those of immunohistochemistry.

Conclusions: Abnormal expression of AChE, α7nAChR and NF-κB in placenta may be associated with preeclampsia. Cho­linergic anti-inflammatory pathway may play an important role in the pathogenesis of preeclampsia.

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Keywords

preeclampsia; cholinergic anti-inflammatory pathway; AChE; α7nAChR; NF-κB

About this article
Title

Placental expression of AChE, α7nAChR and NF-κB in patients with preeclampsia

Journal

Ginekologia Polska

Issue

Vol 89, No 5 (2018)

Article type

Research paper

Pages

249-255

Published online

2018-05-30

Page views

4930

Article views/downloads

1390

DOI

10.5603/GP.a2018.0043

Pubmed

30084476

Bibliographic record

Ginekol Pol 2018;89(5):249-255.

Keywords

preeclampsia
cholinergic anti-inflammatory pathway
AChE
α7nAChR
NF-κB

Authors

Linmei Zheng
Lei Shi
Zhongyi Zhou
Xiaoju Chen
Li Wang
Zhicheng Lu
Rong Tang

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