open access

Vol 89, No 3 (2018)
Research paper
Published online: 2018-03-30
Get Citation

Maternal placi protein levels in early- and late-onset preeclampsia

Mujde Can Ibanoglu1, A. Seval Ozgu-Erdinc2, Dilek Uygur2
·
Pubmed: 29664550
·
Ginekol Pol 2018;89(3):147-152.
Affiliations
  1. Dr Nafiz Körez Sincan State Hospital, Ankara, Turkey
  2. Dr Zekai Tahir Burak Women’s Health Care, Education And Research Hospital, Ankara, Turkey

open access

Vol 89, No 3 (2018)
ORIGINAL PAPERS Obstetrics
Published online: 2018-03-30

Abstract

Objectives: The objective of this study was to determine the maternal PLAC1 protein levels in early and late onset preec­lampsia.

Material and methods: A total of 135 pregnant women were included in the study, of which 55 were at < 34 weeks of gesta­tion and 80 were at ≥ 34 weeks of gestation, between June and November 2014 were recruited in this case control study.

Results: Analysis of maternal serum PLAC1 levels did not reveal any significant differences between early onset PE and controls (p = 0.422). However, late onset PE patients exhibited significantly elevated levels of PLAC1, in comparison with healthy controls (p = 0.026). The difference in PLAC1 levels between early onset PE and late onset PE was also significant (p = 0.001). Area under ROC curve of PLAC1 for early and late onset PE was 0.563 and 0.646 with p values of 0.422 and 0.026 respectively. Area under ROC curve of PLAC1 in PE was 0.613 with p value = 0.024. The cutoff value for PLAC1 was 6.19 ng/mL with sensitivity: 56% (95% CI 44.1–67.3) and specificity: 63 %; (95% CI 49.9–75.1) and diagnostic odds ratio: 2.2 (95% CI 1.1–4.4) (p value = 0.037). The cutoff value for PLAC1 was 7.2 ng/mL with sensitivity: 43% (95% CI 31.5–54.6) and specificity: 78% (95% CI 65.5–87.5) and diagnostic odds ratio: 2.69 (95% CI 1.25–5.79) (p value = 0.016)

Conclusion: In conclusion, the results of the current study showed that PLAC1 protein levels were significantly elevated in pregnant women with late onset PE in comparison with healthy control group.

Abstract

Objectives: The objective of this study was to determine the maternal PLAC1 protein levels in early and late onset preec­lampsia.

Material and methods: A total of 135 pregnant women were included in the study, of which 55 were at < 34 weeks of gesta­tion and 80 were at ≥ 34 weeks of gestation, between June and November 2014 were recruited in this case control study.

Results: Analysis of maternal serum PLAC1 levels did not reveal any significant differences between early onset PE and controls (p = 0.422). However, late onset PE patients exhibited significantly elevated levels of PLAC1, in comparison with healthy controls (p = 0.026). The difference in PLAC1 levels between early onset PE and late onset PE was also significant (p = 0.001). Area under ROC curve of PLAC1 for early and late onset PE was 0.563 and 0.646 with p values of 0.422 and 0.026 respectively. Area under ROC curve of PLAC1 in PE was 0.613 with p value = 0.024. The cutoff value for PLAC1 was 6.19 ng/mL with sensitivity: 56% (95% CI 44.1–67.3) and specificity: 63 %; (95% CI 49.9–75.1) and diagnostic odds ratio: 2.2 (95% CI 1.1–4.4) (p value = 0.037). The cutoff value for PLAC1 was 7.2 ng/mL with sensitivity: 43% (95% CI 31.5–54.6) and specificity: 78% (95% CI 65.5–87.5) and diagnostic odds ratio: 2.69 (95% CI 1.25–5.79) (p value = 0.016)

Conclusion: In conclusion, the results of the current study showed that PLAC1 protein levels were significantly elevated in pregnant women with late onset PE in comparison with healthy control group.

Get Citation

Keywords

pregnancy, maternal serum, PLAC1 protein, Preeclampsia

About this article
Title

Maternal placi protein levels in early- and late-onset preeclampsia

Journal

Ginekologia Polska

Issue

Vol 89, No 3 (2018)

Article type

Research paper

Pages

147-152

Published online

2018-03-30

Page views

1348

Article views/downloads

1100

DOI

10.5603/GP.a2018.0025

Pubmed

29664550

Bibliographic record

Ginekol Pol 2018;89(3):147-152.

Keywords

pregnancy
maternal serum
PLAC1 protein
Preeclampsia

Authors

Mujde Can Ibanoglu
A. Seval Ozgu-Erdinc
Dilek Uygur

References (28)
  1. American College of Obstetricians and Gynecologists, Task Force on Hypertension in Pregnancy. Hypertension in pregnancy. Report of the American College of Obstetricians and Gynecologists’ Task Force on Hypertension in Pregnancy. Obstet Gynecol. 2013; 122(5): 1122–1131.
  2. Hutcheon JA, Lisonkova S, Joseph KS. Epidemiology of pre-eclampsia and the other hypertensive disorders of pregnancy. Best Pract Res Clin Obstet Gynaecol. 2011; 25(4): 391–403.
  3. Cunningham FG, Leveno KJ, Bloom SL, Spong CY, Dashe JS, Hoffman BL, Casey BM, Sheffield JS. Hypertensive Disorders. In: Williams Obstetrics. 24th Edition. McGraw-Hill, New York 2014: 728–779.
  4. Say L, Chou D, Gemmill A, et al. Global causes of maternal death: a WHO systematic analysis. Lancet Glob Health. 2014; 2(6): e323–e333.
  5. Valensise H, Vasapollo B, Gagliardi G, et al. Early and late preeclampsia: two different maternal hemodynamic states in the latent phase of the disease. Hypertension. 2008; 52(5): 873–880.
  6. von Dadelszen P, Magee LA, Roberts JM. Subclassification of preeclampsia. Hypertens Pregnancy. 2003; 22(2): 143–148.
  7. Jackman SM, Kong X, Fant ME, et al. Plac1 (placenta-specific 1) is essential for normal placental and embryonic development. Mol Reprod Dev. 2012; 79(8): 564–572.
  8. Chang WL, Yang Q, Zhang H, et al. Role of placenta-specific protein 1 in trophoblast invasion and migration. Reproduction. 2014; 148(4): 343–352.
  9. World Medical Association. World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects. JAMA. 2013; 310(20): 2191–2194.
  10. Chaiworapongsa T, Chaemsaithong P, Yeo L, et al. Pre-eclampsia part 1: current understanding of its pathophysiology. Nat Rev Nephrol. 2014; 10(8): 466–480.
  11. Vatten LJ, Skjaerven R. Is pre-eclampsia more than one disease? BJOG. 2004; 111(4): 298–302.
  12. Fant M, Farina A, Nagaraja R, et al. PLAC1 (Placenta-specific 1): a novel, X-linked gene with roles in reproductive and cancer biology. Prenat Diagn. 2010; 30(6): 497–502.
  13. Cocchia M, Huber R, Pantano S, et al. PLAC1, an Xq26 gene with placenta-specific expression. Genomics. 2000; 68(3): 305–312.
  14. Fant M, Weisoly DL, Cocchia M, et al. PLAC1, a trophoblast-specific gene, is expressed throughout pregnancy in the human placenta and modulated by keratinocyte growth factor. Mol Reprod Dev. 2002; 63(4): 430–436.
  15. Concu M, Banzola I, Farina A, et al. Rapid clearance of mRNA for PLAC1 gene in maternal blood after delivery. Fetal Diagn Ther. 2005; 20(1): 27–30.
  16. Massabbal E, Parveen S, Weisoly DL, et al. PLAC1 expression increases during trophoblast differentiation: evidence for regulatory interactions with the fibroblast growth factor-7 (FGF-7) axis. Mol Reprod Dev. 2005; 71(3): 299–304.
  17. Silva WA, Gnjatic S, Ritter E, et al. PLAC1, a trophoblast-specific cell surface protein, is expressed in a range of human tumors and elicits spontaneous antibody responses. Cancer Immun. 2007; 7: 18.
  18. Fant M, Barerra-Saldana H, Dubinsky W, et al. The PLAC1 protein localizes to membranous compartments in the apical region of the syncytiotrophoblast. Mol Reprod Dev. 2007; 74(7): 922–929.
  19. Lau TW, Leung TN, Chan LYS, et al. Fetal DNA clearance from maternal plasma is impaired in preeclampsia. Clin Chem. 2002; 48(12): 2141–2146.
  20. Purwosunu Y, Sekizawa A, Okazaki S, et al. Prediction of preeclampsia by analysis of cell-free messenger RNA in maternal plasma. Am J Obstet Gynecol. 2009; 200(4): 386.e1–386.e7.
  21. Purwosunu Y, Sekizawa A, Farina A, et al. Cell-free mRNA concentrations of CRH, PLAC1, and selectin-P are increased in the plasma of pregnant women with preeclampsia. Prenat Diagn. 2007; 27(8): 772–777.
  22. Farina A, Rizzo N, Concu M, et al. Lower maternal PLAC1 mRNA in pregnancies complicated with vaginal bleeding (threatened abortion <20 weeks) and a surviving fetus. Clin Chem. 2005; 51(1): 224–227.
  23. Ng EKO, Tsui NBY, Lau TK, et al. mRNA of placental origin is readily detectable in maternal plasma. Proc Natl Acad Sci U S A. 2003; 100(8): 4748–4753.
  24. DiFederico E, Genbacev O, Fisher SJ. Preeclampsia is associated with widespread apoptosis of placental cytotrophoblasts within the uterine wall. Am J Pathol. 1999; 155(1): 293–301.
  25. Kodama M, Miyoshi H, Fujito N, et al. Plasma mRNA concentrations of placenta-specific 1 (PLAC1) and pregnancy associated plasma protein A (PAPP-A) are higher in early-onset than late-onset pre-eclampsia. J Obstet Gynaecol Res. 2011; 37(4): 313–318.
  26. Zanello M, Sekizawa A, Purwosunu Y, et al. Circulating mRNA for the PLAC1 gene as a second trimester marker (14-18 weeks' gestation) in the screening for late preeclampsia. Fetal Diagn Ther. 2014; 36(3): 196–201.
  27. Fisher SJ. The placental problem: linking abnormal cytotrophoblast differentiation to the maternal symptoms of preeclampsia. Reprod Biol Endocrinol. 2004; 2: 53.
  28. Luttun A, Carmeliet P. Soluble VEGF receptor Flt1: the elusive preeclampsia factor discovered? J Clin Invest. 2003; 111(5): 600–602.

Regulations

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By VM Media Group sp. z o.o., ul. Świętokrzyska 73, 80–180 Gdańsk
tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl