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Vol 89, No 3 (2018)
Research paper
Published online: 2018-03-30
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HSD3B2, HSD17B1, HSD17B2, ESR1, ESR2 and AR expression in infertile women with endometriosis

Maciej Osiński1, Przemysław Wirstlein1, Ewa Wender-Ożegowska1, Mateusz Mikołajczyk1, Paweł Piotr Jagodziński2, Małgorzata Szczepańska1
DOI: 10.5603/GP.a2018.0022
·
Pubmed: 29664547
·
Ginekol Pol 2018;89(3):125-134.
Affiliations
  1. Division of Reproduction, Department of Obstetrics, Gynaecology and Gynaecological Oncology, Poznan, Poland
  2. Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, Poznan, Poland

open access

Vol 89, No 3 (2018)
ORIGINAL PAPERS Gynecology
Published online: 2018-03-30

Abstract

Objectives: The development of endometriosis is associated with changes in the expression of genes encoding the 3β-hydroxysteroid dehydrogenase type II (HSD3B2) and 17β-hydroxysteroid dehydrogenase type II (HSD17B2), estrogen receptors 1 (ESR1) and 2 (ESR2) and the androgen receptor (AR). However, little is known about the expression of HSD3B2, HSD17B1, HSD17B2, ESR1 ESR2 and AR during the endometrial phases in eutopic endometrium from infertile women with endometriosis.

Material and methods: Using RT-qPCR analysis, we assessed the expression of the studied genes in the follicular and luteal phases in eutopic endometrium from fertile women (n = 17) and infertile women (n = 35) with endometriosis.

Results: In the mid-follicular eutopic endometrium, we observed a significant increase in HSD3B2 transcript levels in all infertile women with endometriosis (p = 0.003), in infertile women with stage I/II endometriosis (p = 0.008) and in infertile women with stage III/IV endometriosis (p = 0.009) compared to all fertile women. There was a significant increase in ESR1 tran­scripts in all infertile women with endometriosis (p = 0.008) and in infertile women with stage I/II endometriosis (p = 0.019) and in infertile women with stage III/IV endometriosis (p = 0.023) compared to all fertile women. In the mid-luteal eutopic endometrium, we did not observe significant differences in HSD3B2, HSD17B1, HSD17B2, ESR1, ESR2 and AR transcripts between infertile women with endometriosis and fertile women.

Conclusions: Observed significant increase in HSD3B2 and ESR1 transcripts in follicular eutopic endometrium from infer­tile women with endometriosis may be related to abnormal biological effect of E2 in endometrium, further affecting the development of human embryos.

Abstract

Objectives: The development of endometriosis is associated with changes in the expression of genes encoding the 3β-hydroxysteroid dehydrogenase type II (HSD3B2) and 17β-hydroxysteroid dehydrogenase type II (HSD17B2), estrogen receptors 1 (ESR1) and 2 (ESR2) and the androgen receptor (AR). However, little is known about the expression of HSD3B2, HSD17B1, HSD17B2, ESR1 ESR2 and AR during the endometrial phases in eutopic endometrium from infertile women with endometriosis.

Material and methods: Using RT-qPCR analysis, we assessed the expression of the studied genes in the follicular and luteal phases in eutopic endometrium from fertile women (n = 17) and infertile women (n = 35) with endometriosis.

Results: In the mid-follicular eutopic endometrium, we observed a significant increase in HSD3B2 transcript levels in all infertile women with endometriosis (p = 0.003), in infertile women with stage I/II endometriosis (p = 0.008) and in infertile women with stage III/IV endometriosis (p = 0.009) compared to all fertile women. There was a significant increase in ESR1 tran­scripts in all infertile women with endometriosis (p = 0.008) and in infertile women with stage I/II endometriosis (p = 0.019) and in infertile women with stage III/IV endometriosis (p = 0.023) compared to all fertile women. In the mid-luteal eutopic endometrium, we did not observe significant differences in HSD3B2, HSD17B1, HSD17B2, ESR1, ESR2 and AR transcripts between infertile women with endometriosis and fertile women.

Conclusions: Observed significant increase in HSD3B2 and ESR1 transcripts in follicular eutopic endometrium from infer­tile women with endometriosis may be related to abnormal biological effect of E2 in endometrium, further affecting the development of human embryos.

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Keywords

fertility, endometriosis, genes expression

About this article
Title

HSD3B2, HSD17B1, HSD17B2, ESR1, ESR2 and AR expression in infertile women with endometriosis

Journal

Ginekologia Polska

Issue

Vol 89, No 3 (2018)

Article type

Research paper

Pages

125-134

Published online

2018-03-30

Page views

2440

Article views/downloads

1891

DOI

10.5603/GP.a2018.0022

Pubmed

29664547

Bibliographic record

Ginekol Pol 2018;89(3):125-134.

Keywords

fertility
endometriosis
genes expression

Authors

Maciej Osiński
Przemysław Wirstlein
Ewa Wender-Ożegowska
Mateusz Mikołajczyk
Paweł Piotr Jagodziński
Małgorzata Szczepańska

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