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Impact of experimental diabetes and chronic hypoxia on rat fetal body weight
Affiliations- Chair and Department of Obstetrics and Pathology of Pregnancy, Medical University of Lublin, Poland
- Department of Experimental and Clinical Pharmacology, Medical University of Lublin, Lublin, Poland, 8B Jaczewskiego Street, 20-090 Lublin, Poland
- Department of Pediatric Propedeutics, Medical University of Lublin, Lublin, Poland, 6 Gębali Street, 20-093 Lublin, Poland
- Department of Histology and Embryology, Medical University of Lublin, Lublin, Poland, 11 Radziwiłłowska Street, 20-080 Lublin, Poland
- Department of Epidemiology and Clinical Research Methodology, Medical University of Lublin, Lublin, Poland, 1 Chodźki Street, 20-093 Lublin, Poland
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Abstract
Objectives: The aim of the study is to determine the impact of the experimental diabetes and the chronic hypoxia on pregnancy development and rat fetal body weight.
Material and methods: The experiment was performed on female Wistar rats. Animals were divided into the experimental groups. I — Controls, II — Untreated diabetes, III — Insulin-treated diabetes, IV — No diabetes with chronic hypoxia, V — Untreated diabetes and chronic hypoxia, VI — Insulin- treated diabetes and chronic hypoxia. Diabetes was induced in groups II, III, V and VI with intraperitoneal injection of streptozocin (STZ) at a dose of 40 mg/kg. Chronic hypoxia was induced by placing dams (groups IV, V and VI) in conditions of 10.5% oxygen and 89.5%. Insulin was administered subcutaneously at the dose of 9 IU/kg. Starting from the 6th day after STZ injection and chronic hypoxia conditions animals were caged together for 12 hours for 3 consecutive days to ensure fertilization. On day 21 of gestation the animals were decapitated, the fetuses were removed and weighted.
Results: Mean fetal body weight in separate groups were: I — 5.38 g, II — 6.04g, III — 5.32g, IV— 5.56 g, V — 3.45 g, VI — 6.23 g.
Conclusions: Pre-existing type 1 diabetes does not affect fetal body weight compared to healthy newborn control rats. Prolonged hypoxia does not impact on fetal body weight. Chronic hypoxia during pregnancy complicated with untreated type 1 diabetes mellitus leads to significant reduction of fetal body weight. Insulin treatment reversed the detrimental effect of chronic hypoxia on fetal development.
Abstract
Objectives: The aim of the study is to determine the impact of the experimental diabetes and the chronic hypoxia on pregnancy development and rat fetal body weight.
Material and methods: The experiment was performed on female Wistar rats. Animals were divided into the experimental groups. I — Controls, II — Untreated diabetes, III — Insulin-treated diabetes, IV — No diabetes with chronic hypoxia, V — Untreated diabetes and chronic hypoxia, VI — Insulin- treated diabetes and chronic hypoxia. Diabetes was induced in groups II, III, V and VI with intraperitoneal injection of streptozocin (STZ) at a dose of 40 mg/kg. Chronic hypoxia was induced by placing dams (groups IV, V and VI) in conditions of 10.5% oxygen and 89.5%. Insulin was administered subcutaneously at the dose of 9 IU/kg. Starting from the 6th day after STZ injection and chronic hypoxia conditions animals were caged together for 12 hours for 3 consecutive days to ensure fertilization. On day 21 of gestation the animals were decapitated, the fetuses were removed and weighted.
Results: Mean fetal body weight in separate groups were: I — 5.38 g, II — 6.04g, III — 5.32g, IV— 5.56 g, V — 3.45 g, VI — 6.23 g.
Conclusions: Pre-existing type 1 diabetes does not affect fetal body weight compared to healthy newborn control rats. Prolonged hypoxia does not impact on fetal body weight. Chronic hypoxia during pregnancy complicated with untreated type 1 diabetes mellitus leads to significant reduction of fetal body weight. Insulin treatment reversed the detrimental effect of chronic hypoxia on fetal development.
Keywords
diabetes, hypoxia, body weight, fetus
About this articleTitle
Impact of experimental diabetes and chronic hypoxia on rat fetal body weight
Journal
Issue
Article type
Research paper
Pages
20-24
Published online
2018-01-31
Page views
1661
Article views/downloads
1311
DOI
Pubmed
Bibliographic record
Ginekol Pol 2018;89(1):20-24.
Keywords
diabetes
hypoxia
body weight
fetus
Authors
Katarzyna Karwasik-Kajszczarek
Iwona Chmiel-Perzyńska
Jacek Marcin Robak
Aleksandra Billewicz-Kraczkowska
Agnieszka Pedrycz
Agata Smoleń
Janusz Jacek Kraczkowski
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