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Vol 88, No 10 (2017)
Research paper
Published online: 2017-10-31
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Mig-7 expression and vasculogenic mimicry in malignant ovarian tumors

Artur Czekierdowski1, Sylwia Czekierdowska1, Norbert Stachowicz2, Tomasz Łoziński3, Grzegorz Gurynowicz4
DOI: 10.5603/GP.a2017.0100
·
Pubmed: 29192416
·
Ginekol Pol 2017;88(10):552-561.
Affiliations
  1. Department of Gynecological Oncology and Gynecology, Staszica 16, 20-081 Lublin, Poland
  2. Department of Epidemiology and Clinical Research Methodology, ul.Chodźki 1, 20-093 Lublin, Poland
  3. Department of Obstetrics and Gynecology ,Pro Familia Hospital, ul.Witolda 6B, 35-302 Rzeszów, Poland
  4. Department of Perinatology and Gynecology, Poznan University of Medical Sciences, Polna St. 33, 60-535 Poznań, Poland

open access

Vol 88, No 10 (2017)
ORIGINAL PAPERS Gynecology
Published online: 2017-10-31

Abstract

Objectives: To investigate the possible association of vasculogenic mimicry (VM), VE-cadherin and MIG-7 expression with clinicopathological features of women with malignant ovarian masses.

Material and methods: VM was studied with the PAS reaction and VE-cadherin was assessed with immunohistochemistry in 108 women with malignant ovarian tumors. Additionally, quantitative expression of MIG-7 mRNA was performed in 52 ovarian cancers with qRT-PCR.

Results: VM was found in 48/108 cases (44%), more often in higher FIGO stage tumors (83% cases; 40 vs. 8; p = 0.01). High expression of VE-cadherin was present in 37% of all ovarian masses. Ovarian tumors without VM more often expressed low levels of VE-cadherin than tumors where VM was found (37.6% vs.14.6%). No expression or very low expression of MIG-7 mRNA was found in all normal ovarian tissues and in 32 cancer samples. Median RQ of MIG-7 mRNA in tumor samples was higher than in normal ovarian tissue (RQ = 0.29 vs. RQ = 0.05, respectively; p < 0.005) and higher than in non-malignant ovarian masses (0.98 vs. 0.05 respectively; p = 0.03). Expression of MIG-7 mRNA was significantly correlated with VM (p = 0.039). In tumors with PAS-positive structures median RQ MIG-7 mRNA was higher than in tumors with PAS-negative findings (1.89 vs. 0.13 respectively). VE-cadherin expression was more frequently found in tumors where MIG-7 mRNA was present (p = 0.004).

Conclusions: Vasculogenic mimicry exists in malignant ovarian tumors and advanced clinical stages of malignancy are accompanied by a high incidence of VM formation. MIG-7 mRNA and VE-cadherin expression may serve as additional molecular markers of VM in ovarian malignancies.

Abstract

Objectives: To investigate the possible association of vasculogenic mimicry (VM), VE-cadherin and MIG-7 expression with clinicopathological features of women with malignant ovarian masses.

Material and methods: VM was studied with the PAS reaction and VE-cadherin was assessed with immunohistochemistry in 108 women with malignant ovarian tumors. Additionally, quantitative expression of MIG-7 mRNA was performed in 52 ovarian cancers with qRT-PCR.

Results: VM was found in 48/108 cases (44%), more often in higher FIGO stage tumors (83% cases; 40 vs. 8; p = 0.01). High expression of VE-cadherin was present in 37% of all ovarian masses. Ovarian tumors without VM more often expressed low levels of VE-cadherin than tumors where VM was found (37.6% vs.14.6%). No expression or very low expression of MIG-7 mRNA was found in all normal ovarian tissues and in 32 cancer samples. Median RQ of MIG-7 mRNA in tumor samples was higher than in normal ovarian tissue (RQ = 0.29 vs. RQ = 0.05, respectively; p < 0.005) and higher than in non-malignant ovarian masses (0.98 vs. 0.05 respectively; p = 0.03). Expression of MIG-7 mRNA was significantly correlated with VM (p = 0.039). In tumors with PAS-positive structures median RQ MIG-7 mRNA was higher than in tumors with PAS-negative findings (1.89 vs. 0.13 respectively). VE-cadherin expression was more frequently found in tumors where MIG-7 mRNA was present (p = 0.004).

Conclusions: Vasculogenic mimicry exists in malignant ovarian tumors and advanced clinical stages of malignancy are accompanied by a high incidence of VM formation. MIG-7 mRNA and VE-cadherin expression may serve as additional molecular markers of VM in ovarian malignancies.

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Keywords

ovarian cancer, Mig-7,VE-cadherin, vasculogenic mimicry

About this article
Title

Mig-7 expression and vasculogenic mimicry in malignant ovarian tumors

Journal

Ginekologia Polska

Issue

Vol 88, No 10 (2017)

Article type

Research paper

Pages

552-561

Published online

2017-10-31

Page views

1506

Article views/downloads

1334

DOI

10.5603/GP.a2017.0100

Pubmed

29192416

Bibliographic record

Ginekol Pol 2017;88(10):552-561.

Keywords

ovarian cancer
Mig-7
VE-cadherin
vasculogenic mimicry

Authors

Artur Czekierdowski
Sylwia Czekierdowska
Norbert Stachowicz
Tomasz Łoziński
Grzegorz Gurynowicz

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