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The MMP-9/TIMP-1 imbalance and the reduced level of TGF-β in the cervical area of amniotic membrane is a possible risk factor of PROM and premature labor — proof-of-concept study
Affiliations- Department of Histology and Embryology, Center for Biostructure Research, Medical University of Warsaw, Warsaw, Poland
- 1st Department of Obstetrics and Gynecology, Medical University of Warsaw, Poland
open access
Abstract
Objectives: To assess the level MMP-9, TIMP-1 and TGF-β in placental and cervical region of amniotic membranes derived from at-term, pre-term and PROM deliveries.
Material and methods: 14 amniotic membranes have been assessed; the quantitative analysis of MMP-9, TGF-β and TIMP-1 was assayed using respective Quantikine Immunoassay Kit.
Results: The MMP-9 level in PROM samples was similar to the level of MMP-9 in at-term membranes and comparable between the cervical and placental region of these membranes. The concentration of TGF-β and TIMP-1 was decreased in the cervical area of AM derived from deliveries complicated with PROM.
Conclusion: The MMP9/TIMP-1 imbalance, as well as the reduced level of TGF-β may be possible risk factors of pre-term labor and PROM.
Abstract
Objectives: To assess the level MMP-9, TIMP-1 and TGF-β in placental and cervical region of amniotic membranes derived from at-term, pre-term and PROM deliveries.
Material and methods: 14 amniotic membranes have been assessed; the quantitative analysis of MMP-9, TGF-β and TIMP-1 was assayed using respective Quantikine Immunoassay Kit.
Results: The MMP-9 level in PROM samples was similar to the level of MMP-9 in at-term membranes and comparable between the cervical and placental region of these membranes. The concentration of TGF-β and TIMP-1 was decreased in the cervical area of AM derived from deliveries complicated with PROM.
Conclusion: The MMP9/TIMP-1 imbalance, as well as the reduced level of TGF-β may be possible risk factors of pre-term labor and PROM.
Keywords
amniotic membrane, PROM, pre-mature labor, TGF-β
About this articleTitle
The MMP-9/TIMP-1 imbalance and the reduced level of TGF-β in the cervical area of amniotic membrane is a possible risk factor of PROM and premature labor — proof-of-concept study
Journal
Issue
Article type
Research paper
Pages
379-384
Published online
2017-07-31
Page views
1654
Article views/downloads
1279
DOI
Pubmed
Bibliographic record
Ginekol Pol 2017;88(7):379-384.
Keywords
amniotic membrane
PROM
pre-mature labor
TGF-β
Authors
Małgorzata Litwiniuk
Małgorzata Radowicka
Alicja Krejner
Mirosław Wielgoś
Tomasz Grzela
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