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TLR family gene expression in relation to the HIF1α and the VEGFR pathway activation in endometrial cancer
, 2, 1, 1, 1, 2
Affiliations- Clinical Department of Oncological Gynecology, II Department of Gynecology and Obstetrics, Medical University of Lodz, Poland, Poland
- Chair of Cytobiochemistry, University of Łodz, Pomorska 141/143, 90-236 Lodz, Poland
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Abstract
Introduction: Malignant neoplasm of the endometrium is the most common malignant neoplasm of the female reproductive system. Toll Like Receptors (TLR) play a significant role in innate and late-immunity against infections or damaged tissues. TLRs are also involved in the development of tumors in their natural microenvironment. TLRs play an important role in angiogenesis, necessary for survival and growth of the tumor. Hypoxia playing a critical role in angiogenesis, carcinogenesis, tumor progression and distant metastasis is primarily mediated through hypoxia inducible factors (HIFs). Vascular endothelial growth factor family proteins (VEGF) are also strongly involved in tumor angiogenesis and their action is strongly associated with TLR receptors.
Objectives: The aim of the study was to correlate the expression of selected TLRs and VEGFR’s as well as HIF1α with clinicopathological data of endometrial cancer patients. Material and methods: 123 neoplastic endometrial samples were included in the study. 51 samples of healthy endometrium served as control. The expression of TLR1, TLR2, TLR3, TLR4, VEGFR1 and VEGFR2, VEGF-A and HIF1α was examined after RNA isolation at the mRNA level by Real Time-PCR.
Results: We have noted a significant correlation between the expression of selected TLR and VEGFR’s and clinical stage as well as pathological grading of endometrial cancer.
Conclusions: Received correlations confirm a significant contribution of some TLR expression and the receptor for VEGF in the pathogenesis of epithelial endometrial cancer.
Abstract
Introduction: Malignant neoplasm of the endometrium is the most common malignant neoplasm of the female reproductive system. Toll Like Receptors (TLR) play a significant role in innate and late-immunity against infections or damaged tissues. TLRs are also involved in the development of tumors in their natural microenvironment. TLRs play an important role in angiogenesis, necessary for survival and growth of the tumor. Hypoxia playing a critical role in angiogenesis, carcinogenesis, tumor progression and distant metastasis is primarily mediated through hypoxia inducible factors (HIFs). Vascular endothelial growth factor family proteins (VEGF) are also strongly involved in tumor angiogenesis and their action is strongly associated with TLR receptors.
Objectives: The aim of the study was to correlate the expression of selected TLRs and VEGFR’s as well as HIF1α with clinicopathological data of endometrial cancer patients. Material and methods: 123 neoplastic endometrial samples were included in the study. 51 samples of healthy endometrium served as control. The expression of TLR1, TLR2, TLR3, TLR4, VEGFR1 and VEGFR2, VEGF-A and HIF1α was examined after RNA isolation at the mRNA level by Real Time-PCR.
Results: We have noted a significant correlation between the expression of selected TLR and VEGFR’s and clinical stage as well as pathological grading of endometrial cancer.
Conclusions: Received correlations confirm a significant contribution of some TLR expression and the receptor for VEGF in the pathogenesis of epithelial endometrial cancer.
Keywords
endometrial cancer; TLR family; VEGF; VEGR2; VEGFR1; HIF1-α
About this articleTitle
TLR family gene expression in relation to the HIF1α and the VEGFR pathway activation in endometrial cancer
Journal
Issue
Article type
Research paper
Pages
439-446
Published online
2020-08-31
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1190
Article views/downloads
1019
DOI
Pubmed
Bibliographic record
Ginekol Pol 2020;91(8):439-446.
Keywords
endometrial cancer
TLR family
VEGF
VEGR2
VEGFR1
HIF1-α
Authors
Katarzyna M. Wojcik-Krowiranda
Ewa Forma
Andrzej Bienkiewicz
Lukasz Cwonda
Joanna Wronska-Stefaniak
Magdalena Brys
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