open access

Vol 91, No 8 (2020)
ORIGINAL PAPERS Gynecology
Published online: 2020-08-31
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TLR family gene expression in relation to the HIF1α and the VEGFR pathway activation in endometrial cancer

Katarzyna M. Wojcik-Krowiranda, Ewa Forma, Andrzej Bienkiewicz, Lukasz Cwonda, Joanna Wronska-Stefaniak, Magdalena Brys
DOI: 10.5603/GP.2020.0073
·
Pubmed: 32902840
·
Ginekol Pol 2020;91(8):439-446.

open access

Vol 91, No 8 (2020)
ORIGINAL PAPERS Gynecology
Published online: 2020-08-31

Abstract

Introduction: Malignant neoplasm of the endometrium is the most common malignant neoplasm of the female reproductive system. Toll Like Receptors (TLR) play a significant role in innate and late-immunity against infections or damaged tissues. TLRs are also involved in the development of tumors in their natural microenvironment. TLRs play an important role in angiogenesis, necessary for survival and growth of the tumor. Hypoxia playing a critical role in angiogenesis, carcinogenesis, tumor progression and distant metastasis is primarily mediated through hypoxia inducible factors (HIFs). Vascular endothelial growth factor family proteins (VEGF) are also strongly involved in tumor angiogenesis and their action is strongly associated with TLR receptors.
Objectives: The aim of the study was to correlate the expression of selected TLRs and VEGFR’s as well as HIF1α with clinicopathological data of endometrial cancer patients. Material and methods: 123 neoplastic endometrial samples were included in the study. 51 samples of healthy endometrium served as control. The expression of TLR1, TLR2, TLR3, TLR4, VEGFR1 and VEGFR2, VEGF-A and HIF1α was examined after RNA isolation at the mRNA level by Real Time-PCR.
Results: We have noted a significant correlation between the expression of selected TLR and VEGFR’s and clinical stage as well as pathological grading of endometrial cancer.
Conclusions: Received correlations confirm a significant contribution of some TLR expression and the receptor for VEGF in the pathogenesis of epithelial endometrial cancer.

Abstract

Introduction: Malignant neoplasm of the endometrium is the most common malignant neoplasm of the female reproductive system. Toll Like Receptors (TLR) play a significant role in innate and late-immunity against infections or damaged tissues. TLRs are also involved in the development of tumors in their natural microenvironment. TLRs play an important role in angiogenesis, necessary for survival and growth of the tumor. Hypoxia playing a critical role in angiogenesis, carcinogenesis, tumor progression and distant metastasis is primarily mediated through hypoxia inducible factors (HIFs). Vascular endothelial growth factor family proteins (VEGF) are also strongly involved in tumor angiogenesis and their action is strongly associated with TLR receptors.
Objectives: The aim of the study was to correlate the expression of selected TLRs and VEGFR’s as well as HIF1α with clinicopathological data of endometrial cancer patients. Material and methods: 123 neoplastic endometrial samples were included in the study. 51 samples of healthy endometrium served as control. The expression of TLR1, TLR2, TLR3, TLR4, VEGFR1 and VEGFR2, VEGF-A and HIF1α was examined after RNA isolation at the mRNA level by Real Time-PCR.
Results: We have noted a significant correlation between the expression of selected TLR and VEGFR’s and clinical stage as well as pathological grading of endometrial cancer.
Conclusions: Received correlations confirm a significant contribution of some TLR expression and the receptor for VEGF in the pathogenesis of epithelial endometrial cancer.

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Keywords

endometrial cancer; TLR family; VEGF; VEGR2; VEGFR1; HIF1-α

About this article
Title

TLR family gene expression in relation to the HIF1α and the VEGFR pathway activation in endometrial cancer

Journal

Ginekologia Polska

Issue

Vol 91, No 8 (2020)

Pages

439-446

Published online

2020-08-31

DOI

10.5603/GP.2020.0073

Pubmed

32902840

Bibliographic record

Ginekol Pol 2020;91(8):439-446.

Keywords

endometrial cancer
TLR family
VEGF
VEGR2
VEGFR1
HIF1-α

Authors

Katarzyna M. Wojcik-Krowiranda
Ewa Forma
Andrzej Bienkiewicz
Lukasz Cwonda
Joanna Wronska-Stefaniak
Magdalena Brys

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