open access

Vol 91, No 6 (2020)
ORIGINAL PAPERS Gynecology
Published online: 2020-06-30
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A preliminary study on the immune responses of HPV16-E7 by combined intranasal immunization with lymphotoxin

Ya-Hong Liu, Hai-Lin Chen, Bo-Qun Xu, Kuang Wei, Xiao-Yan Ying
DOI: 10.5603/GP.2020.0055
·
Pubmed: 32627150
·
Ginekol Pol 2020;91(6):301-307.

open access

Vol 91, No 6 (2020)
ORIGINAL PAPERS Gynecology
Published online: 2020-06-30

Abstract

Objectives: Human papillomavirus (HPV) ranks the first cause of cervical cancer. Cervical cancer has high prevalence rates
in women around the world. The HPV-E7 oncoprotein is expressed in cervical cancer and is a target of developing immunotherapies
against HPV-associated tumors. However, the antigenicity of this protein is low. Due to this reason, potent
adjuvants are required to enhance its therapeutic efficacy. This preliminary study aims to evaluate whether lymphotoxin
(LT) could act as an effective immune adjuvant for HPV infection in mice models.
Material and methods: Intranasal immunization was used to explore the effect of HPV-E7 and/or LT immune response.
After the third intranasal immunization, the titer for the HPV-E7 antibody was detected in serum and vaginal washing fluid.
Also, we assessed the expression of chemokine ligand 13 (CXCL13) and Peripheral Node Addressin (PNAd) in the lymph
nodes after intranasal immunization with immunohistochemical analysis.
Results: compared to HPV-E7 immunization, intranasal immunization with HPV-E7 plus LT significantly increased HPV-E7-specific
serum IgG and vaginal IgA titers. Furthermore, the combined use of HPV-E7 and LT strongly induced E7-specific CTL
responses.
Conclusions: LT can be effective for intranasal immunized HPV-E7 to improve E7-specific immune responses to HPV infection.
It is new approach to eradicate chronic HPV infection capable of inducing an effective anti-infection method.

Abstract

Objectives: Human papillomavirus (HPV) ranks the first cause of cervical cancer. Cervical cancer has high prevalence rates
in women around the world. The HPV-E7 oncoprotein is expressed in cervical cancer and is a target of developing immunotherapies
against HPV-associated tumors. However, the antigenicity of this protein is low. Due to this reason, potent
adjuvants are required to enhance its therapeutic efficacy. This preliminary study aims to evaluate whether lymphotoxin
(LT) could act as an effective immune adjuvant for HPV infection in mice models.
Material and methods: Intranasal immunization was used to explore the effect of HPV-E7 and/or LT immune response.
After the third intranasal immunization, the titer for the HPV-E7 antibody was detected in serum and vaginal washing fluid.
Also, we assessed the expression of chemokine ligand 13 (CXCL13) and Peripheral Node Addressin (PNAd) in the lymph
nodes after intranasal immunization with immunohistochemical analysis.
Results: compared to HPV-E7 immunization, intranasal immunization with HPV-E7 plus LT significantly increased HPV-E7-specific
serum IgG and vaginal IgA titers. Furthermore, the combined use of HPV-E7 and LT strongly induced E7-specific CTL
responses.
Conclusions: LT can be effective for intranasal immunized HPV-E7 to improve E7-specific immune responses to HPV infection.
It is new approach to eradicate chronic HPV infection capable of inducing an effective anti-infection method.

Get Citation

Keywords

human papillomavirus; HPV-E7; lymphotoxin; intranasal immunization; immunotherapy; lymphocyte homing

About this article
Title

A preliminary study on the immune responses of HPV16-E7 by combined intranasal immunization with lymphotoxin

Journal

Ginekologia Polska

Issue

Vol 91, No 6 (2020)

Pages

301-307

Published online

2020-06-30

DOI

10.5603/GP.2020.0055

Pubmed

32627150

Bibliographic record

Ginekol Pol 2020;91(6):301-307.

Keywords

human papillomavirus
HPV-E7
lymphotoxin
intranasal immunization
immunotherapy
lymphocyte homing

Authors

Ya-Hong Liu
Hai-Lin Chen
Bo-Qun Xu
Kuang Wei
Xiao-Yan Ying

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