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Endometrial microbiota — do they mean more than we have expected?
Affiliations- Department of Obstetrics and Gynecological Nursing, Faculty of Health Sciences, Medical University of Gdansk, Poland, Poland
- Invicta Fertility Clinic, Gdansk, Poland
- I Department of Obstetrics and Gynecology, Center of Postgraduate Medical Education, Warsaw, Poland
- Department of Gynecological Endocrinology, Medical University of Warsaw, Poland
open access
Abstract
Low biomass microbiome has an increasing importance in today’s fertility studies. There are more and more indications
for incorporating upper gynecological tract microbiome content in patients diagnostic and in vitro fertilization process, as
doing so may help to evaluate chances for a positive outcome. An abnormal endometrial microbiota has been associated
with implantation failure, pregnancy loss, and other gynecological and obstetrical conditions. Furthermore it has been
shown, that using molecular methods in addition to routine diagnostics may help diagnose chronic endometritis or even
indicate cancerogenic changes. Understanding the significance of microbiome in endometrium may completely change
therapeutic approach in treatment of this part of reproductive tract. Next generation sequencing (NGS) has allowed to
isolate culturable and unculturable bacteria from female reproductive tract and is a cheaper and quicker alternative for
other widely known and used methods.
Abstract
Low biomass microbiome has an increasing importance in today’s fertility studies. There are more and more indications
for incorporating upper gynecological tract microbiome content in patients diagnostic and in vitro fertilization process, as
doing so may help to evaluate chances for a positive outcome. An abnormal endometrial microbiota has been associated
with implantation failure, pregnancy loss, and other gynecological and obstetrical conditions. Furthermore it has been
shown, that using molecular methods in addition to routine diagnostics may help diagnose chronic endometritis or even
indicate cancerogenic changes. Understanding the significance of microbiome in endometrium may completely change
therapeutic approach in treatment of this part of reproductive tract. Next generation sequencing (NGS) has allowed to
isolate culturable and unculturable bacteria from female reproductive tract and is a cheaper and quicker alternative for
other widely known and used methods.
Keywords
endometrium; microbiota; reproductive health; next generation sequencing
About this articleTitle
Endometrial microbiota — do they mean more than we have expected?
Journal
Issue
Article type
Review paper
Pages
45-48
Published online
2020-01-31
Page views
2704
Article views/downloads
2796
DOI
Pubmed
Bibliographic record
Ginekol Pol 2020;91(1):45-48.
Keywords
endometrium
microbiota
reproductive health
next generation sequencing
Authors
Natalia Mlodzik
Krzysztof Lukaszuk
Wlodzimierz Sieg
Grzegorz Jakiel
Roman Smolarczyk
References (36)- Tissier H. Recherches sur la flore intestinale des nourrissons : état normal et pathologique. G Carré et C Naud, Paris 1900.
- Weiss JE, Rettger LF. Taxonomic Relationships of Lactobacillus Bifidus (B. Bifidus Tissier) and Bacteroides Bifidus. Journal of Infectious Diseases. 1938; 62(1): 115–120.
- Jiménez E, Marín ML, Martín R, et al. Is meconium from healthy newborns actually sterile? Res Microbiol. 2008; 159(3): 187–193.
- Jiménez E, Fernández L, Marín ML, et al. Isolation of commensal bacteria from umbilical cord blood of healthy neonates born by cesarean section. Curr Microbiol. 2005; 51(4): 270–274.
- Bearfield C, Davenport ES, Sivapathasundaram V, et al. Possible association between amniotic fluid micro-organism infection and microflora in the mouth. BJOG. 2002; 109(5): 527–533.
- Steel JH, Malatos S, Kennea N, et al. Bacteria and inflammatory cells in fetal membranes do not always cause preterm labor. Pediatr Res. 2005; 57(3): 404–411.
- Jagodzinski A, Zielinska E, Laczmanski L, et al. The early years of life. Are they influenced by our microbiome? Ginekol Pol. 2019; 90(4): 228–232.
- Brunelli R, Papi M, Arcovito G, et al. Globular structure of human ovulatory cervical mucus. The FASEB Journal. 2007; 21(14): 3872–3876.
- Møller BR, Kristiansen FV, Thorsen P, et al. Sterility of the uterine cavity. Acta Obstet Gynecol Scand. 1995; 74(3): 216–219.
- Gajer P, Brotman RM, Guoyun B, et al. Temporal Dynamics of the Human Vaginal Microbiota. Sci Transl Med. 2012; 4(132): 132ra52.
- Ding T, Schloss PD. Dynamics and associations of microbial community types across the human body. Nature. 2014; 509(7500): 357–360.
- Chen C, Song X, Wei W, et al. The microbiota continuum along the female reproductive tract and its relation to uterine-related diseases. Nat Commun. 2017; 8(1): 875.
- Drancourt M, Bollet C, Carlioz A, et al. 16S Ribosomal DNA Sequence Analysis of a Large Collection of Environmental and Clinical Unidentifiable Bacterial Isolates. Journal of Clinical Microbiology. 2000; 38(10): 3623–3630.
- Gill SR, Pop M, Deboy RT, et al. Metagenomic analysis of the human distal gut microbiome. Science. 2006; 312(5778): 1355–1359.
- Salipante SJ, Sengupta DJ, Rosenthal C, et al. Rapid 16S rRNA next-generation sequencing of polymicrobial clinical samples for diagnosis of complex bacterial infections. PLoS One. 2013; 8(5): e65226.
- Sunagawa S, Mende D, Zeller G, et al. Metagenomic species profiling using universal phylogenetic marker genes. Nature Methods. 2013; 10(12): 1196–1199.
- Franasiak J, Scott R. Introduction. Fertility and Sterility. 2015; 104(6): 1341–1343.
- Moreno I, Codoñer FM, Vilella F, et al. Evidence that the endometrial microbiota has an effect on implantation success or failure. Am J Obstet Gynecol. 2016; 215(6): 684–703.
- Wira CR, Fahey JV, Rodriguez-Garcia M, et al. Regulation of mucosal immunity in the female reproductive tract: the role of sex hormones in immune protection against sexually transmitted pathogens. Am J Reprod Immunol. 2014; 72(2): 236–258.
- Frew L, Stock SJ. Antimicrobial peptides and pregnancy. Reproduction. 2011; 141(6): 725–735.
- King AE. Presence of secretory leukocyte protease inhibitor in human endometrium and first trimester decidua suggests an antibacterial protective role. Molecular Human Reproduction. 2000; 6(2): 191–196.
- Givan AL, White HD, Stern JE, et al. Flow cytometric analysis of leukocytes in the human female reproductive tract: comparison of fallopian tube, uterus, cervix, and vagina. Am J Reprod Immunol. 1997; 38(5): 350–359.
- Koedooder R, Mackens S, Budding A, et al. Identification and evaluation of the microbiome in the female and male reproductive tracts. Hum Reprod Update. 2019; 25(3): 298–325.
- Benner M, Ferwerda G, Joosten I, et al. How uterine microbiota might be responsible for a receptive, fertile endometrium. Hum Reprod Update. 2018; 24(4): 393–415.
- Fanchin R, Harmas A, Benaoudia F, et al. Microbial flora of the cervix assessed at the time of embryo transfer adversely affects in vitro fertilization outcome. Fertility and Sterility. 1998; 70(5): 866–870.
- Selman H, Mariani M, Barnocchi N, et al. Examination of bacterial contamination at the time of embryo transfer, and its impact on the IVF/pregnancy outcome. J Assist Reprod Genet. 2007; 24(9): 395–399.
- Moore D, Soules M, Klein N, et al. Bacteria in the transfer catheter tip influence the live-birth rate after in vitro fertilization. Fertility and Sterility. 2000; 74(6): 1118–1124.
- Salim R. Bacterial colonization of the uterine cervix and success rate in assisted reproduction: results of a prospective survey. Human Reproduction. 2002; 17(2): 337–340.
- Mitchell CM, Haick A, Nkwopara E, et al. Colonization of the upper genital tract by vaginal bacterial species in nonpregnant women. Am J Obstet Gynecol. 2015; 212(5): 611.e1–611.e9.
- Franasiak JM, Werner MD, Juneau CR, et al. Endometrial microbiome at the time of embryo transfer: next-generation sequencing of the 16S ribosomal subunit. J Assist Reprod Genet. 2016; 33(1): 129–136.
- Fang RL, Chen LX, Shu WS, et al. Barcoded sequencing reveals diverse intrauterine microbiomes in patients suffering with endometrial polyps. Am J Transl Res. 2016; 8(3): 1581–1592.
- Haahr T, Jensen JS, Thomsen L, et al. Abnormal vaginal microbiota may be associated with poor reproductive outcomes: a prospective study in IVF patients. Hum Reprod. 2016; 31(4): 795–803.
- Moreno I, Cicinelli E, Garcia-Grau I, et al. The diagnosis of chronic endometritis in infertile asymptomatic women: a comparative study of histology, microbial cultures, hysteroscopy, and molecular microbiology. Am J Obstet Gynecol. 2018; 218(6): 602.e1–602.e16.
- Yang TK, Chung CJ, Chung SD, et al. Risk of Endometrial Cancer in Women With Pelvic Inflammatory Disease: A Nationwide Population-Based Retrospective Cohort Study. Medicine (Baltimore). 2015; 94(34): e1278.
- Sharma H, Tal R, Clark NA, et al. Microbiota and pelvic inflammatory disease. Semin Reprod Med. 2014; 32(1): 43–49.
- Elinav E, Nowarski R, Thaiss CA, et al. Inflammation-induced cancer: crosstalk between tumours, immune cells and microorganisms. Nat Rev Cancer. 2013; 13(11): 759–771.
